Latest technologies from University of Pennsylvania

An Organometallic Inhibitor for Glycogen Synthase Kinase 3

jharris@ctt.upenn.edu — Thu, 07 Jun 2012 07:17:37 GMT

TechID: Q3604 Since the mid-1990s there has been a near exponential rise in the level of protein kinase and glycogen synthase kinase-3 (GSK-3) directed research. Consequently the therapeutic potential of protein kinase inhibitors in general and GSK-3 inhibitors in particular has become a rapidly growing area of interest to the pharmaceutical industry. There is particularly strong evidence to support the development of GSK-3 inhibitors as: 1) inhibitors of neuronal apoptosis and neurological decline in stroke patients; 2) therapeutics targeted to bipolar disorder and depression; ...

New Tandem Epoxidation and Oxidation Reactions

jharris@ctt.upenn.edu — Thu, 07 Jun 2012 07:16:56 GMT

TechID: Q3444 Medications sold as single enantiomers comprise 50 of the 100 top selling drugs (e.g. Lipitor, Zyprexa, Nexium, Plavid, Zoloft), and currently represent a $172 billion industry, up from $133 billion in 2000. By 2008, chiral drugs will account for 40% of emerging and updated drug strategies. The precursors of these medications are chiral substances of high optical purity, which constitute an important class of starting materials for organic and medicinal chemists. Using the stereochemistry of these materials to control generation of subsequent stereogenic centers allow...

Method for Synthesis of Novel Antibiotics

jharris@ctt.upenn.edu — Thu, 07 Jun 2012 07:14:14 GMT

TechID: O2813 Ramoplanin is among a new class of naturally occurring antibiotics capable of targeting unique enzymatic processes critical for cell wall synthesis in gram-positive bacteria. Ramoplanin is a potent killer of several increasingly prevalent antibiotic resistant species of bacteria including vancomycin-resistant Enterococcus faecium (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Researchers at the University of Pennsylvania have optimized a solid-phase protocol for the complete total synthesis of ramoplanin. This procedure allows for ra...

Catalytic Asymmetric Alkylation of Ketones

jharris@ctt.upenn.edu — Thu, 07 Jun 2012 07:14:07 GMT

TechID: O2788 Medications sold as single enantiomers comprise 50 of the 100 top selling drugs, and currently represent a $133 billion industry, from $90 billion in 1999. The precursors of these medications are chiral substances of high optical purity, which constitute an important class of starting materials for organic and medicinal chemists. Using the stereochemistry of these materials to control generation of subsequent stereogenic centers allows for the preparation of biologically and medicinally important target molecules as single enantiomers. Organic compounds of very high op...

Synthesis and Use of Reagents for Improved DNA Lipofection and/or Slow Release Prodrug Therapies

jharris@ctt.upenn.edu — Thu, 07 Jun 2012 07:09:53 GMT

TechID: N2612 Researchers at the University of Pennsylvania have developed a versatile new class of pharmacologically-active molecules with multiple applications. In transfection experiments, the molecules deliver genes to cultured cells with considerably more success than commercially available reagents. In addition to their gene delivery capabilities, the structure of the molecules allows them to function as slow-release pro-drugs for local treatment of inflammation. Importantly, this therapeutic effect may synergize with the gene that is delivered. ...

anti-WNV E (mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:57:01 GMT

mono (IgGsb) Clone: 4E1 Applications: ELISA, flow cytometry, IHC, IP, WB Species Reactivity: human Alternative Names: West nile virus envelope protein Immunogen: DNA plasmid encoding WNV prM and E (full length), and an innoculation with soluble E protein Relevant Publications: [ELISA, IHC] - Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, Murtadha MM, Hoxie JA, Doms RW. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005 May 25;336(1):70-82. [Pu...

anti-WNV E (mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:59 GMT

mono (IgG1) Clone: 8B10 Applications: ELISA, IP, IHC, neutrralization assay, WB Species Reactivity: human Alternative Names: West nile virus envelope protein Immunogen: DNA plasmid encoding WNV prM and E (full length), and an innoculation with inactivated virus (8B10) Relevant Publications: [ELISA, IHC, IP, WB,] - Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, Murtadha MM, Hoxie JA, Doms RW. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005 M...

anti-WNV E (mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:58 GMT

mono (IgG2a) Clone: 17D7 Applications: ELISA, IHC, IP, WB Species Reactivity: human Alternative Names: West nile virus envelope protein Immunogen: DNA plasmid encoding WNV prM and E (full length), with booster of formalin inactivated virus Relevant Publications: [ELISA, IHC, IP, WB,] - Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, Murtadha MM, Hoxie JA, Doms RW. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005 May 25;336(1):70-82. [Pubmed: ...

anti-HA95

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:55 GMT

Polyclonal Applications: WB, IP Species Reactivity: Human Alternative Names: homologus to AKA95, ALAP8L, A kinase anchor protein 8-like, helicase A- binding protein 95 kDa, HAP95, neighbor or A kinase anchoring protein 95, NAKAP, NAKAP95, Immunogen: HA95 peptide (AA 65-85) Relevant Publications: [IP, WB] - Li Y, Kao GD, Garcia BA, Shabanowitz J, Hunt DF, Qin J, Phelan C, Lazar MA. A novel histone deacetylase pathway regulates mitosis by modulating Aurora B kinase activity. Genes Dev. 2006 Sep 15;20(18...

anti-AKAP95

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:54 GMT

Polyclonal Applications: WB, IHC Species Reactivity: Human Alternative Names: A-kinase anchor protein 95kDa, AKAP8 Immunogen: AKAP95 peptide (AA 672-688) Relevant Publications: [IHC, WB] - Li Y, Kao GD, Garcia BA, Shabanowitz J, Hunt DF, Qin J, Phelan C, Lazar MA. A novel histone deacetylase pathway regulates mitosis by modulating Aurora B kinase activity. Genes Dev. 2006 Sep 15;20(18):2566-79. [Pubmed: 16980585] Tech-ID: Y6052

anti-Dicer

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:54 GMT

Monoclonal Clone: 10-35 Applications: WB, IP Species Reactivity: Human Alternative Names: DICER1, DCR1, Dicer, HERNA, KIAA0928, MNG1 Relevant Publications: [IP, WB] - data available Tech-ID: X5971

anti-mili (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:53 GMT

Monoclonal Clone: 17.8 Applications: WB, IP Species Reactivity: Mouse, Xenopus Alternative Names: Piwil2, Mili / PiwiL2 Immunogen: Recombinant GST–Mili Relevant Publications: [IP, WB] - Kirino Y, Kim N, de Planell-Saguer M, Khandros E, Chiorean S, Klein PS, Rigoutsos I, Jongens TA, Mourelatos Z. Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability. Nat Cell Biol. 2009 May;11(5):652-8. [Pubmed: 19377467] [WB] - Kirino Y, Vourekas A, Sayed N, de Lima Alves F,...

anti-arginylated beta actin (anti-R-beta) (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:52 GMT

Polyclonal (IgG) Clone: POLYCLONAL - no clone ID Applications: WB, ELISA Species Reactivity: Mouse, Rat Alternative Names: Beta actin Immunogen: N-terminally arginylated beta actin peptide RDDIAALC Relevant Publications: [ELISA, WB] - data available elF2c2Tech-ID: X5925

anti-TFAM

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:52 GMT

Applications: WB, IP, ELISA, IHC Alternative Names: Transcription factor A, mitochondrial, MtTF1, TCF6, mtTFA Relevant Publications: [ELISA, IHC, IP, WB] - data available Tech-ID: X5906

anti-CD24 (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:51 GMT

Monoclonal (IgM) Clone: J11.D2 Applications: Cytotoxicity assay, Flow cytometry Species Reactivity: Mouse Alternative Names: CD24, CD24/ heat stable antigen (.HSA) Immunogen: i.v. with mouse C3H/HeJ thymocytes Relevant Publications: [Cytotoxicity assay] - Bruce J, Symington FW, McKearn TJ, Sprent J A monoclonal antibody discriminating between subsets of T and B cells. J Immunol. 1981 Dec;127(6):2496-501 [Pubmed: 6975326] [Flow cytometry] - Correa I, Bix M, Liao NS, Zijlstra M, Jaenisch R, Raulet D. M...

anti-CD90.2 (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:50 GMT

Monoclonal (IgM) Clone: j1j.10 Applications: Cytotoxicity assay, Flow cytometry, Cell depletion Species Reactivity: Mouse Alternative Names: Thy1, Thy1.2, CD90.2 Immunogen: i.v. with mouse C3H/HeJ thymocytes Relevant Publications: [Cytotoxicity assay] - Bruce J, Symington FW, McKearn TJ, Sprent J A monoclonal antibody discriminating between subsets of T and B cells. J Immunol. 1981 Dec;127(6):2496-501 [Pubmed: 6975326] [Flow cytometry] - Correa I, Bix M, Liao NS, Zijlstra M, Jaenisch R, Raulet...

anti-CD22 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:50 GMT

Monoclonal (IgG1) Clone: Cy34 Applications: IP, Radioimmunoassay, Flow cytometry, ELISA, IF Species Reactivity: Mouse Alternative Names: CD22, Lyb8.2, CD22 Immunogen: i.v. with spleen cells and s.c. with B10.D2 spleen cells Relevant Publications: [Flow cytometry, IP, Radioimmunoassay] - Symington FW, Subbarao B, Mosier DE, Sprent J. Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody. Immunogenetics. 1982;16(5):381-91. [Pubmed: 6984010] [IF, ELISA] - Dromer F...

anti-CdtB (CdtB19D6) (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:49 GMT

Monoclonal Clone: CdtB19D6 Applications: ELISA Species Reactivity: Other Alternative Names: CdtB Immunogen: rCtdB with C-terminal His tag Relevant Publications: [WB] - Shenker BJ, Besack D, McKay T, Pankoski L, Zekavat A, Demuth DR., Actinobacillus actinomycetemcomitans cytolethal distending toxin (Cdt): evidence that the holotoxin is composed of three subunits: CdtA, CdtB, and CdtC., J Immunol. 2004 Jan 1;172(1):410-7. [Pubmed: 14688349] [ELISA] - Additional data available [ICC, FACS] - Boesze-Ba...

anti-CdtC (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:48 GMT

Monoclonal Clone: 6C11 Applications: ELISA Species Reactivity: Other Alternative Names: CdtC Immunogen: CtdC GST fusion protein Relevant Publications: [WB] - Shenker BJ, Besack D, McKay T, Pankoski L, Zekavat A, Demuth DR., Actinobacillus actinomycetemcomitans cytolethal distending toxin (Cdt): evidence that the holotoxin is composed of three subunits: CdtA, CdtB, and CdtC., J Immunol. 2004 Jan 1;172(1):410-7. [Pubmed: 14688349] [ICC, FACS] - Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis ...

anti-CD317 mAb (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:48 GMT

Monoclonal (IgG2a) Clone: 40H2 Applications: WB, IHC Species Reactivity: not known Immunogen: Recombinant extracellular domain of CD317 Relevant Publications: [IHC, WB] - data available Tech-ID: W5539

anti-CD317 mAb (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:47 GMT

Monoclonal (IgG2a) Clone: 55C2 Applications: WB, IHC Species Reactivity: not known Immunogen: Recombinant extracellular domain of CD317 Relevant Publications: [IHC, WB] - data available Tech-ID: W5538

anti-surfactant protein D (AHV-10F6E12-09) (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:46 GMT

Monoclonal Clone: AHV-10F6E12-09 Applications: ELISA, WB, IHC, FACS Species Reactivity: Mouse, Human Alternative Names: Sftpd, Sftpd, SP-D Immunogen: Murine recombinant SP-D Relevant Publications: [ELISA, FACS, IHC, WB] - data available Tech-ID: W5520

anti-IGHMBP2 (mAb11-24) (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:46 GMT

Monoclonal (IgG1) Clone: 2B1 Applications: IP, IF, WB Species Reactivity: Human Alternative Names: IGHMBP2 Immunogen: Amino-terminal portion of human IGHMBP2 Relevant Publications: [ICC, WB] - de Planell-Saguer M, Schroeder DG, Rodicio MC, Cox GA, Mourelatos Z., Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery., Hum Mol Genet. 2009 Jun 15;18(12):2115-26. Epub 2009 Mar 19. [Pubmed: 19299493] [IF, IP, WB] - Additional data available (unpublished) Tech-ID: W5517

anti-death receptor-5 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:45 GMT

Monoclonal (IgG1) Clone: YM366 Applications: FACS, ELISA, WP, IP Species Reactivity: Human Alternative Names: TNFRSF10B, TRAIL Receptor 2 / Death Receptor 5 / DR5 Immunogen: Full-length extracellular domain of the human DR5 Relevant Publications: [FACS, ELISA] - Ma Y, Yang D, Chen Y., Analysis of TRAIL receptor expression using anti-TRAIL death receptor-5 monoclonal antibodies., Chin Med J (Engl). 2003 Jun;116(6):947-50. [Pubmed: 12877814] [FACS, ELISA, IP, WB] - Additional data available ...

anti-N-Cor (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:44 GMT

Isotype: IgG Applications: WB, ChIP Species Reactivity: Murine, Human Alternative Names: Nuclear receptor co-repressor 1, thyroid-hormone and retinoic-acid- associated co-repressor-1 (TRAC-1) Immunogen: N-Cor peptide (1944-2453) Relevant Publications: [WB] - Huang EY, Zhang J, Miska EA, Guenther MG, Kouzarides T, Lazar MA. Nuclear receptor corepressors partner with class II histone deacetylases in a Sin3-independent repression pathway. Genes Dev. 2000 Jan 1;14(1):45-54. [Pubmed: 10640275] [ChIP] - You SH, ...

anti-Resistin (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:44 GMT

Species Reactivity: Human Alternative Names: adipose tissue-specific secretory factor (ADSF), C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) Tech-ID: V5220

anti-AMPKb

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:43 GMT

Polyclonal Alternative Names: AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase Tech-ID: V5186

anti-AMPKa 2 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:42 GMT

Polyclonal Applications: WB, IP Species Reactivity: Murine Alternative Names: AMP-activted protein kinase, 5' adenosine monophosphate-activated protein kinase Immunogen: AMPKa2 peptide (N-term) Relevant Publications: [IP, WB] - Mu J, Brozinick JT Jr, Valladares O, Bucan M, Birnbaum MJ. A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 2001 May;7(5):1085-94. [Pubmed: 11389854] Tech-ID: V5185

anti-AMPKa 2

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:42 GMT

Polyclonal Applications: IP Species Reactivity: Human Alternative Names: AMP-activted protein kinase, 5' adenosine monophosphate-activated protein kinase Immunogen: AMPKa2 peptide (C-term) Relevant Publications: [IP] - Fujii N, Hayashi T, Hirshman MF, Smith JT, Habinowski SA, Kaijser L, Mu J, Ljungqvist O, Birnbaum MJ, Witters LA, Thorell A, Goodyear LJ. Exercise induces isoform-specific increase in 5'AMP-activated protein kinase activity in human skeletal muscle. Bioch [Pubmed: 10891387] Tech-ID: V5184

anti-AMPKpT172 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:41 GMT

Polyclonal Applications: WB, IP Species Reactivity: Murine Alternative Names: AMP-activted protein kinase, 5' adenosine monophosphate-activated protein kinase Immunogen: AMPKpT172 peptide Relevant Publications: [IP, WB] - Mu J, Brozinick JT Jr, Valladares O, Bucan M, Birnbaum MJ. A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell. 2001 May;7(5):1085-94. [Pubmed: 11389854] [WB] - Choi SL, Kim SJ, Lee KT, Kim J, Mu J, Birnbaum MJ, ...

anti-IRAP

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:40 GMT

Alternative Names: Insuin-Responsive Aminopeptidase Tech-ID: V5053

anti-JBP1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:40 GMT

Monoclonal Clone: 6G8 Applications: ELISA, IF, IP, WB Species Reactivity: Human Alternative Names: PRMT5 Immunogen: Monoclonal anti-JBP1 antibody (6G8) was prepared by immunizing Balb/C mice with a C-terminal domain of JBP1 (amino acid 284–637) purified on glutathione-Sepharose and cleaved off the beads with TEV protease. Relevant Publications: [IP, WB] - Friesen WJ, Wyce A, Paushkin S, Abel L, Rappsilber J, Mann M, Dreyfuss G., A novel WD repeat protein component of the methylosome bin...

anti-FXR1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:39 GMT

Monoclonal Clone: 6BG10 Applications: ELISA, ICC, IP, IF, WB Species Reactivity: Human Alternative Names: FXR1P Immunogen: Recombinant protein corresponding to human FXR1 Relevant Publications: [ICC] - Battle DJ, Kasim M, Wang J, Dreyfuss G., SMN-independent subunits of the SMN complex. Identification of a small nuclear ribonucleoprotein assembly intermediate., J Biol Chem. 2007 Sep 21;282(38):27953-9. Epub 2007 Jul 19. [Pubmed: 17640873] [WB] - Xu XL, Zong R, Li Z, Biswas MH, Fang Z, Nelson DL, ...

anti-gemin 7 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:39 GMT

Monoclonal Clone: 6E2 Applications: ELISA, ICC, IP, IF, WB Species Reactivity: Human Alternative Names: SIP3 Immunogen: Recombinant protein corresponding to human Gemin7 Relevant Publications: [WB] - Battle DJ, Kasim M, Wang J, Dreyfuss G., SMN-independent subunits of the SMN complex. Identification of a small nuclear ribonucleoprotein assembly intermediate., J Biol Chem. 2007 Sep 21;282(38):27953-9. Epub 2007 Jul 19. [Pubmed: 17640873] [ICC, WB] - Hao le T, Fuller HR, Lam le T, Le TT, Burghes ...

anti-gemin 5 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:38 GMT

Monoclonal Clone: 10G11 Applications: ICC, IF, IP, WB Species Reactivity: Human Alternative Names: gemin-5 Immunogen: Raised against a His-tagged recombinant protein corresponding to full- length Gemin5 of human origin. Relevant Publications: [ICC, WB] - Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G., Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins., J Biol Chem. 2002 Feb 15;277(7):5631-6. Epub 2001 Nov 19. [Pubmed: 11714716] [ICC, I...

anti-Gemin 4 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:37 GMT

Monoclonal Clone: 17D10 Applications: WB Species Reactivity: Human Alternative Names: HC56, HCAP1, HHRF-1, p97 Immunogen: Histidine-tagged recombinant protein corresponding to human Gemin4 Relevant Publications: [WB] - Pillai RS, Artus CG, Filipowicz W., Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis., RNA. 2004 Oct;10(10):1518-25. Epub 2004 Aug 30. [Pubmed: 15337849] [WB] - Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, Abel L, Rapps...

anti-SMN (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:36 GMT

Monoclonal (IgG1) Clone: 2B1 Applications: ICC, IP, WB Species Reactivity: Human Alternative Names: gemin 1, SMN1 Immunogen: Anti-SMN antibody (2B1) was prepared by immunizing A/J mice with His6- tagged SMN protein (human) purified from Ni+ chelation. Hybridoma production, screening and ascites fluid production were then performed. Relevant Publications: [ICC, IP, WB] - Liu Q, Dreyfuss G., A novel nuclear structure containing the survival of motor neurons protein., EMBO J. 1996 Jul 15;15...

anti-Y14 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:36 GMT

Monoclonal (IgG1) Clone: 1F12 Applications: WB Species Reactivity: Human Alternative Names: RNA binding motif protein 8A, RBM8A Immunogen: Purified human Y14 Relevant Publications: [WB] - Kim VN, Yong J, Kataoka N, Abel L, Diem MD, Dreyfuss G., The Y14 protein communicates to the cytoplasm the position of exon-exon junctions., EMBO J. 2001 Apr 17;20(8):2062-8. [Pubmed: 11296238] [WB] - Dostie J, Dreyfuss G., Translation is required to remove Y14 from mRNAs in the cytoplasm., Curr Biol. 2002 Jul 9;12(...

anti-Gemin 2/Sip-1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:35 GMT

Monoclonal Clone: 2S7 Applications: ICC, IP, WB Species Reactivity: Human, Xenopus Alternative Names: SIP1, gemin 2 Immunogen: Anti-SIP1 antibodies 2S7 were prepared by immunizing Balb/C mice with His6-tag SMN protein purified from nickel chelation chromatography using a Novagen His-Bind buffer kit. Relevant Publications: [ICC, IP, WB] - Liu Q, Fischer U, Wang F, Dreyfuss G., The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceo...

anti-eIF4A3 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:34 GMT

Monoclonal Clone: 3F1 Applications: ICC, IP, WB Species Reactivity: Human Alternative Names: DEAD box protein 48 Immunogen: Mouse anti-eIF4A3 monoclonal antibody 3F1 was prepared by immunizing BALB/c mice with full-length recombinant GSTeIF4A3. Relevant Publications: [WB] - Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S., Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsens...

anti-Importin Beta-1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:33 GMT

Monoclonal (IgG3) Clone: 3H14 Applications: ELISA, WB Species Reactivity: Human Alternative Names: KPNB1 Immunogen: Recombinant protein corresponding to human Importin beta-1. Relevant Publications: [ELISA, WB] - data available Tech-ID: Q3267

anti-Importin alpha-1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:32 GMT

Monoclonal (IgG1) Clone: 8G5 Applications: ELISA, WB Species Reactivity: Human Alternative Names: KPNA2 Immunogen: Recombinant protein corresponding to human Importin subunit alpha-1. Relevant Publications: [ELISA, WB] - data available Tech-ID: Q3266

anti-Exportin T (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:32 GMT

Monoclonal (IgG1) Clone: LOS1 Applications: ELISA, WB Species Reactivity: Human Alternative Names: XPOT, XPO3 Immunogen: Full length Exportin T protein. Relevant Publications: [ELISA, WB] - data available Tech-ID: Q3265

anti-TAP (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:31 GMT

Monoclonal (IgG1) Clone: 53H8 Applications: ELISA, IF, IP, WB Species Reactivity: Human Alternative Names: TAP Immunogen: TAP Relevant Publications: [WB] - Kim VN, Kataoka N, Dreyfuss G, Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex., Science. 2001 Sep 7;293(5536):1832-6. [Pubmed: 11546873] [WB] - Kataoka N, Diem MD, Kim VN, Yong J, Dreyfuss G., Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent ex...

anti-NF (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:30 GMT

Monoclonal (RMO 270 IgG2ak, RMO 44 IgG1k, RMO 24 IgG1k, RMO 281 IgG1k, RMO 14.9 IgG1, RMdO-20 IgG1k) Clone: RMO 270, RMO 44, RMO 24, RMO 281, RMO 14.9, RMdO-20, RMO 26, RMO 108, RMO 55, RMO 45, TA 51, RMO 254, HO 14, RMO 8, RMO 281, RMO 34, RMO 217, RMO 304, RMO 81, RMO 87 Applications: WB, IH, Flow cytometry Species Reactivity: RMO 270 Human, RMO 44 human, lamprey, Rat, RMO 24 human, rat, RMO 281 human, RMO 14.9 human, Mouse, rat, RMdO-20 human, hamster, bovine, nouse, rat, rabbit, squid Alternative Nam...

anti-tau (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:30 GMT

Monoclonal (Tau-14 IgG1k, Tau-46 IgG1, PHF-6 (Tau-231) IgG1, PHF-13 (Tau-396) IgG2b) Clone: Tau 14, Tau 46, PHF-6, PHF 13 Applications: IH, WB, IB, IF Species Reactivity: Tau 14 Human, Tau 46 human, Mouse, rabbit, Rat, xenopus Alternative Names: microtubule associated protien tau Immunogen: Bovine Tau (Tau-14, 46) or paired helical filaments tau preparations from human brain (PHF-6, 13) Relevant Publications: [IB, IF, IH] - Kosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. Epitope...

anti-amyloid (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:29 GMT

Monoclonal (IgG2a) Clone: NAB228 Applications: WB, ELISA, IP, IH Species Reactivity: Human Immunogen: b amyloid peptide AA 1-11 Relevant Publications: [WB] - Lee EB, Skovronsky DM, Abtahian F, Doms RW, Lee VM. Secretion and intracellular generation of truncated Abeta in beta-site amyloid-beta precursor protein-cleaving enzyme expressing human neurons. J Biol Chem. 2003 Feb 14;278(7):4458-66 [Pubmed: 12480937] [IH] - Hamilton R, Patel S, Lee EB, Jackson EM, Lopinto J, Arnold SE, Clark CM, Basil A, Shaw LM, Xie SX, Gr...

anti-PABP (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:28 GMT

Monoclonal (IgG2b) Clone: 10E10 Applications: ELISA, FACS, ICC, IP, WB Species Reactivity: Rabbit, Chicken, Human,Mouse, Xenopus Alternative Names: Poly(A) bindng protein Immunogen: Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI was purified and dialyzed against H2O. The insoluble protein was freeze- dried, emulsified in complete Freud's adjuvant, and injected i.p. in female BALB/c mice. Relevant Publications: [ELISA, FACS, ICC, IP, WB] - Görlach M, Burd CG, Dreyfuss...

anti-hnRNPA1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:27 GMT

Monoclonal (IgG2b) Clone: 9H10 Applications: ELISA, FACS, ICC, IF, IHC, IP, WB Species Reactivity: Human, Mouse Alternative Names: hnRNP-A1 Immunogen: Full length hnRNPA1 native protein (partially purified) from HeLa cells (Human). Relevant Publications: [ELISA, FACS, ICC, IHC, IP, WB] - Burd CG, Dreyfuss G., RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing., EMBO J. 1994 Mar 1;13(5):1197-204. [Pubmed: 7510636] [IP] - Woolley AG, Algie M...

anti-hnRNP-A2/B2 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:26 GMT

Monoclonal (IgG2a) Clone: DP3B3 Applications: ICC, IHC, IP, WB Species Reactivity: Mouse, Human, chicken, canine, Rat, monkey, bovine, hamster Alternative Names: hnRNP-A2/B2 Immunogen: A full-length cDNA encoding hnRNP A2 was cloned into the pET-28a bacterial expression vector , and expression of the encoded protein was induced in Escherichia coli. Purified proteins were dialyzed against phosphate buffer saline (PBS, pH 7.4). Anti-hnRNP Relevant Publications: [ICC, IHC, IP, WB] - Ka...

anti-hnRNP-A1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:26 GMT

Monoclonal (IgG2a) Clone: 4B10 Applications: ELISA, ICC, IP, WB Species Reactivity: Mouse, Rat, Human Alternative Names: hnRNP-A1 Immunogen: The anti-A1 monoclonal antibody 4B 10 was prepared by immunizing a NZB mouse (Frosch et al. 1985) with partially purified HeLa A1. Relevant Publications: [ELISA, ICC, IP, WB] - Piñol-Roma S, Choi YD, Matunis MJ, Dreyfuss G., Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins., Genes Dev. 19...

anti-hnRNP-C1/C2 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:25 GMT

Monoclonal (IgG1) Clone: 4F4 Applications: ELISA, ICC/IF, IP, IHC-P, WB Species Reactivity: Human, Monkey, Hamster, and Chicken Alternative Names: hnRNP-C1/C2 Immunogen: RNP’s eluted from oligo (dT) cellulose column Relevant Publications: [ELISA, ICC/IF, IP, IHC-P, WB] - Choi YD, Grabowski PJ, Sharp PA, Dreyfuss G., Heterogeneous nuclear ribonucleoproteins: role in RNA splicing., Science. 1986 Mar 28;231(4745):1534-9. [Pubmed: 3952495] [IP] - Choi YD, Dreyfuss G., Monoclonal antibody characterizati...

anti-hnRNPJ/K (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:24 GMT

Monoclonal (IgG2b) Clone: 3C2 Applications: Chip-Chip, ELISA, ICC, IF, IP, WB Species Reactivity: Human, Xenopus, Bovine, and Hamster Alternative Names: hnRNPJ/K Immunogen: Antibody was generated by immunizing BALB/c mice against human hnRNPJ/K proteins. Relevant Publications: [ELISA, ICC, IP, WB] - Matunis MJ, Michael WM, Dreyfuss G., Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein., Mol Cell Biol. 1992 Jan;12(1):164-71. [Pubm...

anti-hnRNP-L (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:23 GMT

Monoclonal (IgG1) Clone: 4D11 Applications: ELISA, ICC, IP, WB Species Reactivity: Mouse, Bovine, Human, Monkey, Chicken, and Newt Alternative Names: hnRNP-L Immunogen: Antibody was generated by immunizing BALB/c mice against human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose. Relevant Publications: [ELISA, ICC, IP, WB] - Piñol-Roma S, Swanson MS, Gall JG, Dreyfuss G., A novel heterogeneous nuclear RNP protein with a unique distribution on nascent t...

anti-hnRNP-Q (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:22 GMT

Monoclonal (IgG1) Clone: 18E4 Applications: ELISA, ICC, IP, WB Species Reactivity: Human, Mouse, Xenopus Alternative Names: hnRNP-Q Immunogen: mAb 18E4 against hnRNP Qs was prepared by immunizing mice with human recombinant untagged hnRNP Q protein. Relevant Publications: [ELISA, ICC, IP, WB] - Mourelatos Z, Abel L, Yong J, Kataoka N, Dreyfuss G., SMN interacts with a novel family of hnRNP and spliceosomal proteins., EMBO J. 2001 Oct 1;20(19):5443-52. [Pubmed: 11574476] [ICC, IP, WB] - Barrando...

anti-hnRNP-U (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:22 GMT

Monoclonal (IgG1) Clone: 3G6 Applications: ELISA, ICC, IP, WB Species Reactivity: Human, Monkey, Mouse Alternative Names: hnRNP-U Immunogen: Cross-linked RNPs were isolated from HeLa cells by nuclease digestion of poly(A) purified material. RNPs in complete Freund adjuvant was injected i.p. in BALB/c mice. Hybridomas were made from the spleens after a booster and an i.v. injection. Relevant Publications: [ELISA, ICC, IP, WB] - Dreyfuss G, Choi YD, Adam SA., Characterization of heterogeneo...

anti-Transportin 1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:21 GMT

Monoclonal (IgG1) Clone: D45 Applications: ICC, IP, WB Species Reactivity: Human, Monkey and Rabbit Alternative Names: Transportin 1 Immunogen: The anti-transportin1 monoclonal antibody D45 was obtained by immunization of a BALB/c mouse with human recombinant His-tagged transportin1 Relevant Publications: [ICC, IP, WB] - Siomi MC, Eder PS, Kataoka N, Wan L, Liu Q, Dreyfuss G., Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins., J Cell Biol. 1997 Sep 22;138(6):1181-92. [P...

anti-SMN (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:20 GMT

Monoclonal (IgG1) Clone: 2B1 Applications: IP, WB, ICC Species Reactivity: Human, Mouse, Xenopus Alternative Names: SMN Immunogen: The anti-SMN antibodies 2B1 was prepared by immmunizing A/J mice with His6-tagged human SMN protein. Relevant Publications: [ICC, IP, WB] - Liu Q, Dreyfuss G., A novel nuclear structure containing the survival of motor neurons protein., EMBO J. 1996 Jul 15;15(14):3555-65. [Pubmed: 8670859] [ICC] - Carvalho T, Almeida F, Calapez A, Lafarga M, Berciano MT, Carmo-Fons...

anti-gemin 3 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:20 GMT

Monoclonal Clone: 12H12 Applications: ELISA, ICC, IP, WB Species Reactivity: Human Alternative Names: ddx20, gemin 3 Immunogen: Anti-Gemin3 antibodies 12H12 was prepared by immunizing Balb/C mice with 6His-tag COOH-terminal domain of Gemin3 (from amino acids 368–548 cloned from human) purified from nickel chelation chromatography using Novagen His-Bind buffer kit. Relevant Publications: [ELISA, ICC, IP, WB] - Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G., Gemin...

anti-gemin 2 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:19 GMT

Monoclonal (IgG1) Clone: 2E17 - not 100% sure Applications: IP, WB, ELISA, ICC Species Reactivity: Human, Xenopus Alternative Names: SIP1, gemin 2 Immunogen: Anti-SIP1 antibodies 2S7 and 2K9 were prepared by immunizing Balb/C mice with His6-tag SMN protein purified from nickel chelation chromatography using a Novagen His-Bind buffer kit. Relevant Publications: [ICC, IP, WB] - Liu Q, Fischer U, Wang F, Dreyfuss G., The spinal muscular atrophy disease gene product, SMN, and its associated pro...

anti-dFMR1 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:18 GMT

Monoclonal (IgG1) Clone: 6A15 Applications: ELISA, ICC, IF, IP, WB Species Reactivity: Drosophila Alternative Names: FMR1 Immunogen: The N-terminal 580 amino acids of dFMR1 was cloned into a His tag- containing vector. The induced protein was purified using a His-binding resin column. The purified protein was dialyzed against phosphate-buffered saline (PBS) and used to immunize mice. Relevant Publications: [ELISA, IF, IP, WB] - Wan L, Dockendorff TC, Jongens TA, Dreyfuss G., Characteriz...

anti-human Y14 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:18 GMT

Monoclonal (IgG2b) Clone: 4C4 Applications: ELISA, ICC, IF, IP, WB Species Reactivity: Human Immunogen: GST-tagged Xenopus Y14 Relevant Publications: [ELISA, IF, IP, WB] - Kataoka N, Yong J, Kim VN, Velazquez F, Perkinson RA, Wang F, Dreyfuss G., Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.,Mol Cell. 2000 Sep;6(3):673-82. [Pubmed: 11030346] [ICC, IP, WB] - Ohno M, Segref A, Kuersten S, Mattaj IW., Identity elements used in export of mRNAs., Mol Cell....

anti-Magoh (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:17 GMT

Monoclonal (IgG2a) Clone: 21B12 Applications: ELISA, WB, IP, IHC Species Reactivity: Human Alternative Names: mago-nashi homolog proliferation associated Immunogen: GST-Magoh (human) Relevant Publications: [IP, IHC] - Diem MD, Chan CC, Younis I, Dreyfuss G PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs. Nat Struct Mol Biol. 2007 Dec;14(12):1173-9. [Pubmed: 18026120] [WB] - Inaki M, Kato D, Utsugi T, Onoda F, Hanaoka F, Murakami Y. Genetic analys...

anti-Aly/REF (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:16 GMT

Monoclonal (IgG1) Clone: 11G5 Applications: ELISA, WB, IF, IP, EMSA Species Reactivity: Human, X. laevis Alternative Names: THO complex subunit 4, THOC4 Immunogen: Human Aly/REF protein fused to glutathion S-transferase Relevant Publications: [IP, WB] - Kim VN, Yong J, Kataoka N, Abel L, Diem MD, Dreyfuss G. The Y14 protein communicates to the cytoplasm the position of exon-exon junctions. EMBO J. 2001 Apr 17;20(8):2062-8 [Pubmed: 11296238] [IP, EMSA] - Jiang D, Zhou Y, Moxley RA, Jarrett HW. P...

anti-hnRNP-F/H (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:16 GMT

Monoclonal (IgG1) Clone: 1G11 Applications: ELISA, WB, IF, IP Species Reactivity: Human, bovine, X. laevis, D. melanogaster, S. pombe, S. cerevisiae Alternative Names: heterogeneous nuclear ribonucleoprotein F and H Immunogen: Bacterially expressed hnRNP F Relevant Publications: [IF, IP] - Matunis MJ, Xing J, Dreyfuss G. The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization. Nucleic Acids Res. 1994 Mar 25;22(6):1059-67. [Pubmed: 7512260] ...

anti-hnRNP F (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:15 GMT

Monoclonal (IgG1) Clone: 3H4 Applications: ELISA, WB, IF, IP Species Reactivity: Human Alternative Names: heterogeneous nuclear ribonucleoprotein F, nucleolin-like protein, mcs94-1 Immunogen: Bacterially expressed hnRNP F Relevant Publications: [IF, IP] - Matunis MJ, Xing J, Dreyfuss G. The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization. Nucleic Acids Res. 1994 Mar 25;22(6):1059-67. [Pubmed: 7512260] [WB] - Lefave CV, Squatrito M, Vorlova ...

anti-HveB

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:14 GMT

Monoclonal Alternative Names: herpesvirus entry protein B, poliovirus receptor-related protien 2, PRR2, nectin-2, CD112 Tech-ID: O2890

anti-HSV-1gD (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:14 GMT

Monoclonal (IgG2a) Clone: DL6 Applications: IB, WB, IP, Flow cytometry Immunogen: HSV-1 gD Relevant Publications: [IB] - Eisenberg RJ, Long D, Ponce de Leon M, Matthews JT, Spear PG, Gibson MG, Lasky LA, Berman P, Golub E, Cohen GH. Localization of epitopes of herpes simplex virus type 1 glycoprotein D. J Virol. 1985 Feb;53(2):634-44. [Pubmed: 2578577] [WB] - Cohen GH, Isola VJ, Kuhns J, Berman PW, Eisenberg RJ. Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system...

anti-CD31 (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:13 GMT

Monoclonal (IgG2a) Clone: 390 Applications: Flow cytometry, IP, IH, blocking assays, In vivo lung targeting Species Reactivity: Mouse Alternative Names: platelet endothelial cell adhesion molecule (PECAM-1) Relevant Publications: [WB] - Yan HC, Pilewski JM, Zhang Q, DeLisser HM, Romer L, Albelda SM. Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping. Cell Adhes Commun. 1995 Feb;3(1):45-66. [Pubmed: 7538415] [In vivo lung targeting] - Muzykan...

anti-GPVI (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:12 GMT

Monoclonal (IgG) Clone: Hy101 (or 11A12) Applications: Flow cytometry, WB, binding inhibition in vitro Species Reactivity: Human Alternative Names: GP 6 Immunogen: GPVI R272L expressed on 3T3 cells Relevant Publications: [Flow cytometry, WB] - Chen H, Locke D, Liu Y, Liu C, Kahn ML. The platelet receptor GPVI mediates both adhesion and signaling responses to collagen in a receptor density-dependent fashion. J Biol Chem. 2002 Jan 25;277(4):3011-9 [Pubmed: 11723134] [binding inhibition in vitro] -...

anti-PAPR1 and anti-PAR2 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:12 GMT

Monoclonal (IgG1 or IgG2a (SAM11)) Clone: ATAP2 PC108, PC42, PC143, SPAN 11/12 and SAM11 Applications: ELISA, Flow cytometry, IF Species Reactivity: Human Alternative Names: protease-activated receptor, coagulation factor II (thrombin) receptor (PAR1), coagulation factor II (thrombin)receptor-like 1 (PAR2) Immunogen: PAR1 or PAR2 peptide Relevant Publications: [ELISA, Flow cytometry] - Brass LF, Vassallo RR Jr, Belmonte E, Ahuja M, Cichowski K, Hoxie JA. Structure and function of the human...

anti-a-synuclein (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:11 GMT

Monoclonal (Syn 102 IgG1, Syn 202 IgG2a, Syn 204 IgG2a, Syn 205 IgG1, Syn 207 IgM, Syn 208 IgG2a, Syn 211 IgG1, Syn 214 IgG2a, Syn 303 IgG, Syn 505 IgG, Syn 514 IgG) Clone: Syn 102, Syn 202, Syn 204, Syn 205, Syn 207, Syn 208, Syn 211, Syn 214, LB509, Syn 303, Syn 505, Syn 514, nSyn 14, nSyn 12 Applications: ELISA, WB, IH, immunoEM, IF Species Reactivity: Human, human, Mouse, Rat (Syn 102, 202, 205, 207, 214) Alternative Names: proteins: SNCA, NACP, PARK1, alpha-Synuclein, Non-A beta component of AD a...

anti-LBM180 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:10 GMT

Monoclonal (IgG) Clone: 3C9 Applications: IF, WB, IP, in vitro binding and uptake assays Species Reactivity: Rat Alternative Names: lamellar body membrane 180kDa (LBM180), ATP-binding cassette protein ABCA3 Immunogen: lamellar body membrane fractions Relevant Publications: [IF, WB] - Zen K, Notarfrancesco K, Oorschot V, Slot JW, Fisher AB, Shuman H. Generation and characterization of monoclonal antibodies to alveolar type II cell lamellar body membrane. Am J Physiol. 1998 Jul;275(1 Pt 1):L172-...

anti-Vif (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:10 GMT

Monoclonal (IgG1) Applications: ELISA, WB Species Reactivity: Human Alternative Names: viral infectivity factor Immunogen: His6:Vif fusion protein Relevant Publications: [ELISA, WB] - Simon JH, Southerling TE, Peterson JC, Meyer BE, Malim MH. Complementation of vif-defective human immunodeficiency virus type 1 by primate, but not nonprimate, lentivirus vif genes. J Virol. 1995 Jul;69(7):4166-72. [Pubmed: 7769676] [WB] - Fouchier RA, Simon JH, Jaffe AB, Malim MH. Human immunodeficiency virus type 1 Vif does no...

anti-fusin/CXCR4 (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:09 GMT

Monoclonal (IgG) Clone: 12G5 Applications: infection inhibition in vitro, Flow cytometry, IF, Competition assay Species Reactivity: Human Alternative Names: CXCR4, CD184 Immunogen: CP-MAC-infected Sup-T1 cells Relevant Publications: [Flow cytometry, IF, infection inhibition in vitro] - Endres MJ, Clapham PR, Marsh M, Ahuja M, Turner JD, McKnight A, Thomas JF, Stoebenau-Haggarty B, Choe S, Vance PJ, Wells TN, Power CA, Sutterwala SS, Doms RW, Landau NR, Hoxie JA. CD4-independent infection by HI...

anti-HSV-gD (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:09 GMT

Monoclonal Clone: DL11 Applications: WB Alternative Names: Herpes simplex virus glycoprotein D Immunogen: Immunosorbent-purified gD-1 and gD-2 Relevant Publications: [WB] - Cohen GH, Isola VJ, Kuhns J, Berman PW, Eisenberg RJ. Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing ("native" gel) system of polyacrylamide gel electrophoresis coupled with Western blotting. J Virol. [Pubmed: 2427745] [WB] - Cohen GH, Wilcox WC, Sodora DL, Long D, Levin JZ, Eisenberg RJ. E...

anti-K8 KSHV (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:05 GMT

Monoclonal Applications: IHC, IP, WB Species Reactivity: KSHV virus Alternative Names: K8 Immunogen: GST-K8 alfa fusion protein Relevant Publications: [IHC, IP, WB] - Zhu FX, King SM, Smith EJ, Levy DE, Yuan Y., A Kaposi's sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation., Proc Natl Acad Sci U S A. 2002 Apr 16;99(8): [Pubmed: 11943871] [WB] - Zhu FX, Sathish N, Yuan Y., Antagonism of host antiviral response...

anti-ORF45 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:05 GMT

Polyclonal Applications: IP, WB Species Reactivity: KSHV virus Alternative Names: ORF45 Immunogen: Polyclonal anti-ORF45 antibody was generated with ORF45 protein prepared by using a recombinant baculovirus system. (from ref) Relevant Publications: [IP, WB] - Wang Y, Li H, Tang Q, Maul GG, Yuan Y., Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: involvement of host cellular factors., J Virol. 2008 Mar;82(6):2867-82. Epub 2008 Jan 16. [Pubmed: 18199640] [IP, WB] - Wang Y...

anti-Narf (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:04 GMT

Polyclonal (IgG) Applications: WB Species Reactivity: Human Alternative Names: NARF, Narf, nuclear prelamin A recognition factor, IOP2 Immunogen: Recombinant GST tagged human Narf (382-456) Relevant Publications: [WB] - Song D, Lee FS. A role for IOP1 in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2008 Apr 4;283(14):9231-8 [Pubmed: 18270200] Tech-ID: X5884

anti-Trib2

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:03 GMT

Monoclonal Species Reactivity: Human Alternative Names: TRIB2 Immunogen: Human Trib2, but not Trib1 or Trib3. Tech-ID: X5825

anti-BRCA1 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:03 GMT

Polyclonal (IgG) Alternative Names: BRCA1, BRCAI Tech-ID: X5671

anti-BRCA1 (Chicken)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:02 GMT

Polyclonal (IgG) Alternative Names: BRCA1, BRCAI Tech-ID: X5670

anti-MERIT40 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:01 GMT

Polyclonal (IgG) Applications: WB, IF Species Reactivity: Human Alternative Names: BABAM1, HSPC142, C19orf62, FLJ20571, MERIT40, NBA1 Immunogen: GST-MERIT40 fusion protein Relevant Publications: [IF, WB] - Shao G, Patterson-Fortin J, Messick TE, Feng D, Shanbhag N, Wang Y, Greenberg RA. MERIT40 controls BRCA1- Rap80 complex integrity and recruitment to DNA double-strand breaks. Genes Dev. 2009 Mar 15;23(6):740-54 [Pubmed: 19261746] Tech-ID: X5669

anti-KIAA0157 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:00 GMT

Polyclonal (IgG) Applications: WB Species Reactivity: Human Alternative Names: FAM175B, FAM175B, ABRO1, FLJ22338, KIAA0157 Immunogen: Full-length recombinant KIAA0157 Relevant Publications: [WB] - Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA. Differential regulation of JAMM domain deubiquitinating enzyme activity within the RAP80 complex Biol Chem. 2010 Oct 1;285(40):30971-81. [Pubmed: 20656689] Tech-ID: X5668

anti-Kif17b (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:56:00 GMT

Polyclonal (IgG) Applications: WB, IP Species Reactivity: Mouse Alternative Names: kif17, Neuronal kinesin family member; KIF17 Immunogen: His-tagged sequence of KIF17b amino acids 736-800. Relevant Publications: [IP, WB] - Chennathukuzhi V, Morales CR, El-Alfy M, Hecht NB. The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15566-71 [Pubmed: 14673085] Tech-ID: W5579

anti-Reg3Beta (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:59 GMT

Polyclonal Applications: WB, IHC Species Reactivity: Mouse Alternative Names: Reg3b, HIP, PAP1, Pap, REG-III Immunogen: Peptide RNPSTALDRAFC Relevant Publications: [IHC, WB] - De León DD, Farzad C, Crutchlow MF, Brestelli J, Tobias J, Kaestner KH, Stoffers DA., Identification of transcriptional targets during pancreatic growth after partial pancreatectomy and exendin-4 treatment., Physiol Genomics. 2006 Jan 12;24(2):133-43. [Pubmed: 16410542] Tech-ID: W5578

anti-Reg1 and Reg2 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:58 GMT

Polyclonal Applications: WB Species Reactivity: Mouse Alternative Names: Reg1 and Reg2, RegI, RegII Immunogen: Peptide CKESGTTASNVWT Relevant Publications: [WB] - De León DD, Farzad C, Crutchlow MF, Brestelli J, Tobias J, Kaestner KH, Stoffers DA., Identification of transcriptional targets during pancreatic growth after partial pancreatectomy and exendin-4 treatment., Physiol Genomics. 2006 Jan 12;24(2):133-43. [Pubmed: 16410542] Tech-ID: W5577

anti-CdtA (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:57 GMT

Polyclonal Applications: WB Species Reactivity: Other Alternative Names: CdtA Immunogen: CtdA GST fusion protein Relevant Publications: [WB] - Shenker BJ, Besack D, McKay T, Pankoski L, Zekavat A, Demuth DR., Actinobacillus actinomycetemcomitans cytolethal distending toxin (Cdt): evidence that the holotoxin is composed of three subunits: CdtA, CdtB, and CdtC., J Immunol. 2004 Jan 1;172(1):410-7. [Pubmed: 14688349] [FACS, ICC] - Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, Shenke...

anti-SMRT (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:57 GMT

Monoclonal Clone: SMRT 5.1 Applications: WB Species Reactivity: Human Alternative Names: NCOR2, nuclear receptor corepressor 2 Immunogen: GST–SMRT amino acids 2004–2310 Relevant Publications: [WB] - Huang EY, Zhang J, Miska EA, Guenther MG, Kouzarides T, Lazar MA., Nuclear receptor corepressors partner with class II histone deacetylases in a Sin3-independent repression pathway., Genes Dev. 2000 Jan 1;14(1):45-54. [Pubmed: 10640275] Tech-ID: W5388

anti-ATE1 (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:38 GMT

Monoclonal Clone: 6F11 Applications: WB, IHC Species Reactivity: Mouse, Rat Alternative Names: Ate1 Immunogen: Mix of full-length bacteria expressed ATE1-1 and ATE1-2 Relevant Publications: [IHC, WB] - Kurosaka S, Leu NA, Zhang F, Bunte R, Saha S, Wang J, Guo C, He W, Kashina A. Arginylation-dependent neural crest cell migration is essential for mouse development. PLoS Genet. 2010 Mar 12;6(3):e1000878 [Pubmed: 20300656] Tech-ID: X5924

anti-Abraxas (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:34 GMT

Polyclonal (IgG) Applications: WB Species Reactivity: Human Alternative Names: FAM175A, Abraxas, UNQ496/PRO1013, ABRA1, CCDC98, FLJ11520, FLJ12642, FLJ13614 Immunogen: His-ZZ-Abraxas fusion protein Relevant Publications: [WB] - Shao G, Patterson-Fortin J, Messick TE, Feng D, Shanbhag N, Wang Y, Greenberg RA. MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks. Genes Dev. 2009 Mar 15;23(6):740-54 [Pubmed: 19261746] Tech-ID: X5656

anti-CD317 mAb (Rat)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:31 GMT

Monoclonal (IgG2a) Clone: 44E9 Applications: WB, IHC Species Reactivity: not known Immunogen: Recombinant extracellular domain of CD317 Relevant Publications: [IHC, WB] - data available Tech-ID: W5537

anti-Ago2 (2A8) (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:30 GMT

Monoclonal Clone: 2A8 Applications: WB, IHC, RNA pull down Species Reactivity: Human Alternative Names: EIF2C2, argonaute 2, AGO2, MGC3183, Q10 Immunogen: His-hAgo2 (aa 47-879) Relevant Publications: [IHC, RNA pull down, WB] - Nelson PT, De Planell- Saguer M, Lamprinaki S, Kiriakidou M, Zhang P, O'Doherty U, Mourelatos Z., A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells., RNA. 2007 Oct;13(10):1787-92. [Pubmed: 17720879] ...

anti-H2 complex Ia b/d (Mouse)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:27 GMT

Monoclonal (IgG) Clone: BP107.2.2 Applications: Cytotoxicity assay, FACS, Radioimmunoassay Species Reactivity: Mouse Immunogen: i.v. with ConA stimulated mouse B10.D2 lymph node lymphocytes Relevant Publications: [Cytotoxicity assay] - Symington FW, Sprent J. A monoclonal antibody detecting an Ia specificity mapping in the I-A or I-E subregion. Immunogenetics. 1981;14(1-2):53-61 [Pubmed: 6173320] [FACS] - Correa I, Bix M, Liao NS, Zijlstra M, Jaenisch R, Raulet D. Most gamma delta T cells develop normally in beta...

anti-IscA1 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:24 GMT

Polyclonal (IgG) Applications: WB, IP Species Reactivity: Human Alternative Names: ISCA1, Iron-sulfur cluster assembly 1 homolog Immunogen: Recombinant GST tagged human IscA1 (1-129) Relevant Publications: [IP, WB] - Song D, Tu Z, Lee FS. Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron-sulfur protein biogenesis. J Biol Chem. 2009 Dec 18;284(51):35297- 307 [Pubmed: 19864422] Tech-ID: X5883

anti-IOP1 (Rabbit)

jharris@ctt.upenn.edu — Tue, 03 Jan 2012 08:55:20 GMT

Polyclonal (IgG) Applications: WB, IP Species Reactivity: Mouse, Human Alternative Names: NARFL, IOP1, NARFL, nuclear prelamin A recognition factor-like Immunogen: Recombinant GST tagged human IOP1 (417-476) Relevant Publications: [WB] - Song D, Lee FS. A role for IOP1 in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2008 Apr 4;283(14):9231-8 [Pubmed: 18270200] [IP] - Song D, Tu Z, Lee FS. Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron- su...

Gene Therapy for Leber's Congenital Amaurosis (LCA) and other early Onset Blinding Diseases

jharris@ctt.upenn.edu — Wed, 02 Nov 2011 09:27:43 GMT

TechID: N2514 A recent study published in the New England Journal of Medicine describe results of a clinical trial performed by researchers from The University of Pennsylvania and the Children’s Hospital of Philadelphia, have used gene therapy to safely restore vision in three young adults with Leber congenital amaurosis (LCA) which is a rare form of congenital blindness with no current method of treatment. Although the patients have not achieved normal eyesight, the preliminary results set the stage for further studies of an innovative treatment for this and possibly other...

Novel bisaminoquinoline autophagy inhibitors

jharris@ctt.upenn.edu — Mon, 18 Jul 2011 10:42:00 GMT

NOVEL AUTOPHAGY INHIBITORS Autophagy consists of the sequestration of organelles and proteins in autophagic vesicles (AV), the fusion of AV with lysosomes and the degradation of cargo through the action of acid-dependent lysosomal enzyme. Autophagy allows tumor cells to survive metabolic and therapeutic stresses, and therapy-induced autophagy is a key resistance mechanism to many anti-cancer agents. On the basis of reports that effective autophagy inhibition can be achieved in vivo with the antimalarial drug chloroquine (CQ), a phase III trial in glioblastoma patients treated with ra...

Re-engineered stromal cell-derived Factor-1a (SDF) polypertide analog

jharris@ctt.upenn.edu — Mon, 18 Apr 2011 10:04:37 GMT

Synthetic Peptide Improves Cardiac Remodeling Ischemic heart disease affects a large percentage of our population. Therapy includes a complex cocktail of maintenance drugs that seek to improve cardiac function and relieve symptoms. However, the incidence of morbidity is still high due to this disease, and novel therapies are needed. A novel peptide was developed that promotes vascularization and cardiac remodeling after a heart attack, or after prolonged heart disease. Both in vitro and in vivo studies have been conducted and show promising results.

Liquid Injection Closed Circuit Administration of Sevoflurane by Waters' Canister for Cardioversion

jharris@ctt.upenn.edu — Tue, 17 Aug 2010 11:11:09 GMT

TechID: V4965 PORTABLE ECO-FRIENDLY ANESTHESIA SYSTEM Introduction: Brief yet painful medical procedures need patient sedation; however it may be inefficient and difficult to schedule an operating room that has the large anesthesia equipment required for such procedures. In emergency situations large anesthesia equipment may not be available. Penn inventors have devised a portable eco-friendly anesthesia system to alleviate this problem. The first application of this technology is for cardioversion. Most patients require sedation for cardioversion, typically with intraven...

An Oral Appliance for Prevention of Airway Obstruction During Peroral Endoscopy

jharris@ctt.upenn.edu — Tue, 15 Jun 2010 06:19:01 GMT

TechID: T4412 Background: Many patients require sedation when undergoing an endoscopy procedure and, in some cases respiratory obstruction occurs. When inserted in the mouth, this device advances the lower mandible preventing airway collapse. A central lumen allows endoscope insertion and an ancillary lumen are provided for ventilation with positive airway pressure devices like Continuous Positive Airway Pressure (CPAP) or Pressure Support Ventilation (PSV). Application/Target User: Anesthesiologists and gastroenterologists. Advant...

Human Galactokinase Gene

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:25 GMT

TechID: H1231 Most inherited human metabolic disorders are recessive, many of which have devastating effects such as mental retardation, peripheral nervous system impairment, hearing deficiency, blindness, and organomegaly. Galactokinase deficiency is one of three known forms of galactosemia, where there is enzymatic deficiency in the conversion of galactose to glucose in the body. This causes accumulation of galactose in the body with resulting cellular toxicity in the form of cataracts in newborns and occasional m...

Alicyclic Non-Peptide Peptidomimetics

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:19 GMT

TechID: E903 Peptides are important in many biochemical processes in humans. Two classes of peptides, hormones and neurotransmitters, are controlled by receptor-mediated stimulation of regulatory proteins called G proteins. Examples of G protein-linked receptors include receptors for somatostatin, catecholamines and substance P. Synthetic peptides could mimic or inhibit biological and/or chemical activity of the peptides that bind G protein-linked receptors. However, limited therapeutic applications exist involving peptides due to poor oral bioavailability a...

CYP3A4 NFSE Variant in Human Malignancies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:15 GMT

TechID: L1904 Human diseases, including cancer, are caused by inherited genotypes as well as exposure to environmental agents. Inherited genetic variability may modulate an individual’s response to specific environmental exposures, including carcinogens, compounds found in the diet, and drugs. Hundreds of metabolic enzymes are involved in metabolism of these compounds. One of the most important of these metabolic enzymes is the cytochrome P450 IIIA4, which is encoded by the CYP3A4 gene. In the present invention, a researcher at the University of Pennsylvania descr...

Abnormal Proteins in Alzheimer Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:09 GMT

TechID: D776-CIP The main pathologic signs of Alzheimer’s disease are senile plaques and neurofibrillary tangles, which represent alterations in the cytoskeletal apparatus. The principle structural elements of neurofibrillary tangles are paired helical filaments (PHFs) which in turn are comprised of low relative molecular weight proteins termed tau proteins. University of Pennsylvania researchers have developed antibodies specific to peptide sequences within tau proteins (obtained directly from the brains of patients suffering Alzheimer’s disease). Applications &n...

Ectopic Pregnancy Markers

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:06 GMT

TechID: R3684 An ectopic pregnancy occus when implantation of the embryo occurs outside of the uterus. A major difficulty with diagnosis of ectopic pregnancy is that methods of detection are limited and ultrasound is unreliable in earlist days of gestation. Currently, no serum test exist to distinguish an ectopic pregnancy from a normal intrauterine pregnancy. Investigators at the University of Pennsylvania have collected, catalogued and stored over 1000 samples of which roughly 20% are are from women with an ectopic pregnancy. Blood serum was collected from women with ...

Enzyme-Activated Photodynamic Therapy Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:05 GMT

TechID: P3170 This portfolio of three dockets included novel compound and scheme for utilizing photodynamic therapy (PDT) with both imaging (diagnostic) and therapeutic modalities. This procedure is based on a technology known as FRET (fluorescence resonance energy transfer). For the first technology, "Enzyme-Activated Photodynamic Therapy Agents", with FRET, a fluorophore and a quencher are held in close proximity by an amino acid sequence with a specific protease cleavage site. In the presence of the protease, the amino acid chain is cleaved, releasing the fluorophore from its q...

Folate Receptor Targeted PDT Agent

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:39:03 GMT

TechID: S4263 See parent docket P3170. Applications Advantages

Regulation of the Function of Angiopoietin

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:50 GMT

TechID: O2762 Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are crucial regulators of angiogenesis during both normal development and tumor growth. Ang-1 maintains vascular integrity by mediating signals through the Tie-2 receptor and ultimately promotes the formation of blood vessels. Conversely, Ang-2 plays an antagonistic role of Ang-1 and inhibits angiogenesis. The ratio of Ang-1 to Ang-2 concentration likely serves as an important determinant of angiogenesis. Researchers at the University of Pennsylvania have discovered that Ang-1 is bound to the extracellular...

A Novel Isoform of Glucosamine-6-Sulfatase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:49 GMT

TechID: L2051 Researchers from the University of Pennsylvania have isolated and sequenced a clone from quail cDNA which shows homology to the known human sequence for glucose-6-sulfatase (G6S). Using this quail clone, similar homologous gene sequences have been identified for the first time in C. elegans, Drosophila, and murine DNA. Experiments have demonstrated that G6S is an essential component of specific inductive signaling, which is critical for the development of neural and mesodermal lineages, as well as other lineages in the vertebrate embryo. Applications ...

An Ophthalmic Augmented Reality Environment

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:48 GMT

TechID: J1510 Successful laser treatment of ophthalmic disease depends on identifying precisely treatable lesions. To that end, University of Pennsylvania researchers have developed an augmented reality (AR) environment that increases the identifiability of these lesions and facilitates the discrimination of healthy versus diseased tissue. The AR environment builds a montage of previously stored photographic and angiographic images and compares these images to the real-time images. Applications Advantages

Methods for Modulation of Cholesterol Transport

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:45 GMT

TechID: J1542 Atherosclerosis, the most significant contributing factor to the industrialized world’s leading cause of death, heart disease, is characterized by fatty plaque deposits in blood vessels. The disease risk is directly related to plasma levels of LDL (bad) cholesterol and inversely related to HDL (good) cholesterol. In addition to being deposited in various tissues and fat cells, it has been shown that LDL molecules are internalized at sites of blood vessel injury by so-called foam cells, which contribute to atherogenesis. The w...

Block Co-Polymer Worm Micelles for Drug Delivery and Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:41 GMT

TechID: Q3320 For gene therapy, cancer treatment and other therapeutic applications, there is a need for drug/agent delivering carries that have a long circulation time (few days) in the body and are permeable to cell membranes. Current choices are viruses and/or liposomes (vesicles comprised of natural phospholipids). However, most viruses cause side effects while liposomes are weak, environmentally sensitive and have a short circulation time. Over ten years of research and collaboration among multiple researchers at the University of Pennsylvania have led to the discovery of ...

Modification of Pancreatico-biliary Stents to Facilitate Removal

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:39 GMT

TechID: R3976 Stents are medical devices that are implanted into body cavity passageways such as blood vessels, pancreatic, bile ducts, etc. to maintain or widen lumens through a body cavity. Although some stents may remain in the body for an extended period of time, many are only required for a short period of time and may be removed after several weeks or months. Procedures utilizing endoscopic snares and lassos are commonly used for the removal of stents; however, complications often arise during the removal procedure. For instance, in the case where a removable stent i...

Increased F2-Isoprostanes in Alzheimer's Disease: Evidence for Enhanced Lipid Peroxidation in vivo

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:36 GMT

TechID: K1881 Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive function, as well as by numerous amyloid plaques, neurofibrillary tangles (NFTs) and extensive neuronal loss in the brains of AD patients. Furthermore, it has been suggested that oxidant stress may be of functional importance in the pathogenesis of AD and that the production of reactive oxygen species (ROS) in the brain leads to lipid peroxidation and neuronal degeneration. Efforts to elucidate the role of lipid peroxidation and oxidative stress in ...

Peptide Scaffolds for Gene Transfer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:31 GMT

TechID: L1923 Effective delivery of genes to the nucleus of slow- or non-dividing eukaryotic cells is an important consideration for gene therapy and experiments involving these types of cells. Expression of genes delivered by adenoviral vectors may persist for only a short period of time, and genes delivered by liposome-mediated transfection are frequently not efficiently taken up by the nucleus. Technology developed at the University of Pennsylvania exploits a fragment of a well-characterized nuclear-localized protein, which facilitates nuclear transport. ...

Computer Doll House

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:30 GMT

TechID: I1466 Researchers at the University of Pennsylvania have developed a computer “doll house” that provides a virtual play environment for all children including those with moderate or severe physical and/or neurological impairments. This doll house can be used for imaginary play, as a form of visualization and self-communication without language. By stimulating the cognitive development in disabled children, developmental milestones may be reached earlier. Applications Advantages

A Novel Strategy to Reduce Toxicity of TRAIL to Liver Cells in the Therapy of Human Cancer Patients (Combined into N2508)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:27 GMT

TechID: N2371 Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis (programmed cell death) in various tumor cells but not in nontransformed, normal cells. The susceptibility of tumor cells to TRAIL and an apparent lack of activity in normal cells have led to the development of TRAIL as a chemotherapeutic agent. Recent preclinical trials demonstrated the ability of TRAIL to kill a broad cross section of human tumor cell types without significantly affecting the survival of normal human ...

a-1 Anti-Chymotrypsin Analogues having Elastase Inhibitory Activity

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:22 GMT

TechID: F1058 It is well established that the release of human neutrophil elastase and other neutrophil proteases plays a significant role in mediating inflammation, causing tissue damage in a wide range of diseases. It is thus desirable to develop safe and effective elastase inhibitors and multifunctional proteases inhibitors as therapeutic agents. Antichymotrypsin (ACT) and a1 protease inhibitor (a1-PI) are highly homologous members of the serine protease inhibitor superfamily. Antichymotrypsin, however, has a narrower spectrum of inhibitory activity. Particular...

Novel MRI Coil

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:21 GMT

TechID: D830 Magnetic Resonance Imaging (MRI) systems typically produce a gradient in the magnetic field by winding loops of wire onto the surface of a cylindrical whole-body gradient coil. However, it is difficult to produce a linear gradient in both the longitudinal and transverse axes of the coil. Schenck attempted to address this problem by wrapping a single wire around a coil support structure in a helical path so that the spacing between respective turns decreases in proportion to the distance from the center of the coil. This design, however, is highly dependent ...

Pluripotent C57BL/6 Mouse Embryonic Stem Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:21 GMT

TechID: U4637 Pluripotent mouse embryonic stem (ES) cells are the basis for generating gene targeted mice. To date, the majority of mouse knockouts have been generated using ES cells derived from the 129 mouse strain. However, the C57BL/6 (B6) strain is used, and preferred, experimentally by other fields of study. Moreover, the B6 BAC library was used to sequence the mouse genome. It would therefore be highly beneficial to have an ES cell line derived from the B6 strain. While a handful of B6 ES cells have been generated, these strains fail to demonstrate sufficient robustness for ...

Rubber Tipped Go Around

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:16 GMT

TechID: L2136 Proximal and distal control of blood vessel is essential in vascular surgery. Current techniques require circumferentially dissecting and placement of umbilical tape or silicone vascular loop around the vessel. Using this technique, the surgeon can pull on the encircling loop and apply a vascular clamp or place a tourniquet around the free ends of the loop. To encircle a large vessel, surgeons dissect the visible circumference and then pass a clamp behind the remainder of the vessel. Most surgeons approach this by "spreading", opening and closing clamp repeatedly. Unf...

A Method for Integrating Online and Offline Cryptographic Signatures

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:15 GMT

TechID: L1901 Many electronic security protocols involve the use of private electronic keys to sign messages and public keys to verify signatures. A user’s private key is usually stored on his/her computer for easy access (online signing). However, an intruder may be able to gain access to the computer from the network, obtain the key, and compromise security. Another approach places the key on a separate computer or device that is not connected to the network; messages are signed on the separate computer (offline signing) and the signed messages are maintained on...

Monoclonal Antibodies Against The Herpesvirus Entry Mediator (HveC, PrrI, HlgR, Nectin-1)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:12 GMT

TechID: M2160 More than 80 million people in America are infected with the herpes simplex virus (HSV). The virus enters cells by a multi-step process involving several glycoproteins and one of at least three recently identified proteins expressed at the surface of the host cells. One such protein, HveC/nectin-1 binds to HSV glycoprotein D and mediates HSV entry into cells. Nectin-1 is a member of the nectin family of highly conserved cell adhesion molecules of the immunoglobulin superfamily of proteins and is express...

Development of Replication Competent HIVs Containing Deletions of gp120 Hypervariable Loops

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:08 GMT

TechID: P3070 HIV has unique mechanisms to evade the host immune response. Following exposure to the virus, a host will develop antibodies specific to the HIV envelope protein GP120. Unfortunately, the host antibodies are usually directed at three variable regions of GP120 (V1, V2 and V3 loops) which are hypermutagenic. In addition to altering the antigenic profile of GP120, these hypervariable loops shield highly conserved CD4 and chemokine binding sites on GP120 from the host immune response. For a HIV vaccine to protect the host, it must provoke broadly neutrali...

Protein Kinase Akt2 and Diabetes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:07 GMT

TechID: N2616 The phosphoinositide-dependent serine-threonine molecule kinase Akt (also known as protein kinase B, or PKBeta) has been proposed to be an intermediate in the signaling pathway by which insulin controls both muscle and fat cell glucose uptake as well as hepatic gluconeogenesis. The Akt2 isoform appears to be enhanced in insulin-responsive tissues and has been specifically implicated in the metabolic actions of the human one. Researchers at the University of Pennsylvania have shown that Akt2 null mice are impaired in the ability of insulin to lower blood gl...

SPICE: A Highly Efficient Inhibitor of Human Complement

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:03 GMT

TechID: O2751 Variola virus, the most virulent member of the Orthopoxvirus genus, is known for its historic ravage as the cause of smallpox. By immunizing with closely related but much less virulent vaccinia virus, the disease has been eradicated in human hosts for over two decades. However, uncertainty about the safe storage of remaining stores of variola virus and the sheer risk of having a large worldwide unvaccinated population has led to the conclusion that immunization against smallpox needs to be re-instituted on a vast scale. A University of Pennsylvania r...

Method for Treating or Retarding Pathology in Inherited Kidney Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:00 GMT

TechID: T4446 Dr Jean Bennett and Dr Josh Lipschutz have developed a method for treatment of Polycystic Kidney Disease, (a progressive, genetic disorder of the kidneys) using a non-invasive method to deliver recombinant adeno-associated virus (AAV) carrying corrective genes. Autosomal-dominant polycystic kidney disease (ADPKD) is one of the most common inherited diseases with an incidence of 500,000 in the US and 12 Million patients worldwide. Mutations in the PKD1 and PKD2 genes lead to ADPKD disease. PKD1 account for 85% of cases and mutations in PKD2 account for the remainin...

Exact Half-Pulse Synthesis via the Inverse Scattering Transform

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:38:00 GMT

TechID: Q3344 See parent docket P3041. Applications Advantages

Three Dimensional Fourier Transform, Fast Spin Echo, Black Blood Magnetic Resonance Angiography

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:58 GMT

TechID: D836 Resonance data is produced by generating a slice gradient, generating a z axis slab selective 90° pulse, generating a z axis slab selective 180° pulse, generating a first phase encoding gradient pulse for a particular Ky coordinate, generating a second phase encoding gradient pulse for a particular Kz coordinate, and generating an x frequency encoding gradient and reading echo signal data from the body portion resulting from the 180° pulse. The method is repeated from the step of generating the 180° pulse, wherein the particular Kz coordinate is incremented for e...

Surgical device for conjunctival closure

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:56 GMT

TechID: U4762 Technology Overview: Surgeons in the Ophthalmology department at the University of Pennsylvania have developed a surgical instrument and closure device for use in rapidly closing superficial elastic layer of tissue such as the conjunctiva without external protrusion and associated pain. The device is intended for use as a replacement for sutures and other standard methods for surgical closure in ophthalmic surgery. Technology Description: · Surgical device for the delivery of bioabsorbable staple like clips and methods for ti...

AAV Microutrophin for Duchenne Muscular Dystrophy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:50 GMT

TechID: Q3355 Duchenne Muscular Dystrophy is an X-linked disorder characterized by severe muscle wasting. It affects 1 in every 3500 male births in the US, and currently there is no cure. Duchenne Muscular Dystrophy is caused by the absence of the dystrophin gene, and efforts to replace the gene in animal models of the disorder have been largely unsuccessful due to antigenic responses to dystrophin. A homolog to dystrophin had been suggested for gene therapy, but the large size of the gene makes it intractable for expression in a viral vector system. Researc...

Bi-Nuclear Electronic Conductors/Detectors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:49 GMT

TechID: F1002 Researchers at the University of Pennsylvania have synthesized electrically conducting peptide complexes. Each complex consists of two peptides bound by different metal-containing complexing agents. The complexing agent has oxidation-reduction potential asymmetry to provide the synthetic complex with a preselected electrical characteristic. For example, complexing agents can be selected to provide electrical bias, like a diode.

CDK2 Inducible Clones: Wild Type and Dominant Negative

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:44 GMT

TechID: O2706 DNA synthesis in higher eukaryotes is initiated by activation of cyclin-dependent kinase 2 (Cdk2) shortly before S phase. Cdk2 is a potential target of chemotherapy drugs, so it would be beneficial to have inducible clones of Cdk2 that could be used to develop drug screening assays. Researchers at the University of Pennsylvania have developed tetracycline-inducible Cdk2 cell lines (both wild type and dominant negative). These cell lines can be used to assess the effects of chemotherapy drugs that target Cdk2. They may also be useful in the discovery of onco...

Neuroprotection of Retinal Ganglion Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:43 GMT

TechID: P3124 Glaucoma is characterized by a loss of ganglion cells in the retina (RGCs). As ganglion cell axons form the optic nerve and bring visual information to the brain, their death directly affects visual performance. Ganglion cell loss can continue after pressure has been brought under control resulting in a need for neuroprotection of retinal ganglion cells. This invention is directed to the neuroprotection of the optic nerve and the treatment of glaucoma, specifically, the invention is directed to a method of preventing, inhibiting, decreasing incidence and suppressing...

Panning Phage Display Libraries Using Magnetically-Activated Cell Sorting

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:42 GMT

TechID: K1855 The ability to produce monoclonal antibodies has revolutionized diagnostic and therapeutic medicine. However, for many human applications, it is desirable to produce human antibodies. There remains a need for a method of isolating recombinant proteins, preferably antibodies, which is rapid and economical, and which will provide a vast array of protein-binding proteins useful for diagnostic and therapeutic applications in humans. The present invention developed at the University of Pennsylvania satisfies this need by providing a novel approach to a panning ...

A Method for the Measurement of the Transverse Relaxation Rate R21 in Magnetic Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:40 GMT

TechID: I1429 University of Pennsylvania researchers have developed a technique to measure the reversible transverse relaxation rate, R2', in magnetic resonance imaging. By judicious choice of field gradients and radio frequency (rf) pulses, signals with decay rates of R2, the irreversible transverse relaxation rate, and R2*, the effective transverse relaxation rate, may be isolated, and R2, R2', and R2* images formed. Applications Advantages

Controlling RNA Digestion by RNase H with a Light-Activated DNA Hairpin

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:33 GMT

TechID: S4240 Gene silencing can be achieved by targeting messenger RNA (mRNA) during the transcription process by using ribozymes, complementary RNA and DNA oligonucleotides. Of these molecules, antisense oligodeoxynucleotides (asODNs) have proven to be effective in gene silencing in several experimental systems and are being evaluated for cancer therapeutics. In mammalian cells, asODNs can be designed to bind to a complementary mRNA sequence. Formation of the DNA-mRNA duplex recruits nucleases (RNase H) that degrade the target mRNA strand. In this way, the asODN targets a...

Compstatin Analogs with Improved Activity#3

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:32 GMT

TechID: R3687 The complement system, the first line of immunological defense against foreign pathogens, is comprised of a number of protein components that undergo protein-protein interactions. Functional protein fragments and complexes are generated by sequential and specific proteolytic processing events, several of which involve the pivotal molecule known as C3. The complement component C3 plays a central and pivotal role in the activation of the complement pathways. Although an important line of defense against pathogens, inappropriate activation of complement may lead to...

Polymersomes and Related Encapsulating Membranes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:27 GMT

TechID: M2182 For gene therapy, cancer treatment and other therapeutic applications, there is a need for drug/agent delivering carries that have a long circulation time (few days) in the body and are permeable to cell membranes. Current choices are viruses and/or liposomes (vesicles comprised of natural phospholipids). However, most viruses cause side effects while liposomes are weak, environmentally sensitive and have a short circulation time. Over ten years of research and collaboration among multiple researchers at the University of Pennsylvania have led to the discovery of ...

Directed Vascular Expression of the Thromboxane A2 Receptor Results in Intrauterine Growth Retardation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:27 GMT

TechID: L2046 Intrauterine growth retardation (IUGR), a common complication of pregnancy, is an important cause of pre- and postnatal mortality. In addition, IUGR appears to influence body size and weight in adolescence and young adulthood. Researchers at the University of Pennsylvania have recently used a novel approach to develop a mouse model of IUGR. This model exhibits an embryonic and placental phenotype, as well as below average birth weight for surviving animals. Also, treatment of these animals with a specific compound completely rescued the phenotype.

Monoclonal Antibodies Against Human HveA

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:24 GMT

TechID: O2790 Herpes simplex virus enters cells by a multi-step process involving several viral envelope glycoproteins. One of the cellular proteins which mediates HSV entry, HveA, is a newly described member of the tumor necrosis factor receptor (TNFR) family of proteins. Researchers at the Univ. of Pennsylvania have previously described 8 monoclonal antibodies raised against a solutble form of HveA as referenced in Whitbeck et al (2001) J. Virology , 75, pp171-180, and have isolated 3 additional monoclonal antibodies CW10-CW12. These antibodies appear to be particularl...

Conjugation of Urokinase Receptor to the Carrier Red Blood Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:22 GMT

TechID: K1811 Fibrinolytic therapy with plasminogen activators (PA) is useful for the treatment of myocardial infarction and, more recently, stroke cases. However, application of PA for dissolution of clots formed or lodged in other vascular areas (e.g. deep veins in the limbs) is limited by its extremely rapid elimination and inactivation which remain major obstacles to safe and effective fibrinolytic therapy. In order to prolong the functional half-life of PA in circulation while limiting their filtration into tissues and the risk of hemorrhage, researchers at the Uni...

Peptide Scaffolds for Gene Transfer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:20 GMT

TechID: L1926 The current use of viral vectors for gene therapy has proved to be expensive. Researchers at the University of Pennsylvania have designed an inexpensive and safe delivering system for selected molecules into the nucleus of eukaryotic cells. The non-viral compound consists of a nuclear targeting peptide conjugated with a “scaffold” peptide to which selected nucleic acid sequences can be attached. To facilitate the transfer of nucleic acid sequences into the nucleus of non-dividing cells, the complex can be incorporated into plasmids and transfected into cells. Th...

Photolithographically defined contact to carbon nanostructures

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:19 GMT

TechID: V5001 The invention is a method to fabricate large arrays of single walled carbon nanotubes (SWCNTs) via a photolithography process that preserves the intrinsic electronic properties of the nanotubes. Photolithography is less costly and time consuming compared electron beam lithography which is commonly used to fabricate SWNT circuits. A limitation of commonly used photolithography process is the formation of photo resist (Shipley 1813) residuals that interact with the carbon nanotubes (via pi-pi stacking) resulting in CNT devices with high resistance. The process disclo...

Resistin as a Marker and Therapeutic Target for Cardiovascular Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:18 GMT

TechID: Q3490 Resistin is a novel adipose secreted protein which promotes insulin resistance in rodents, but the role of human resistin is uncertain because it is produced largely by macrophages. Given species specific, inflammatory cell expression in humans, resistin may provide a unique link between obesity, inflammation and atherosclerosis. Investigators at the University of Pennsylvania have found that plasma resistin levels correlate significantly with markers of inflammation and that resistin levels were associated with coronary atherosclerosis, thereby indicating a unique, s...

A Universal Strategy for the Synthesis of Dendritic Macromolecules from Conventional Monomers by a Combination of Living Polymerization and TERMINI

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:17 GMT

TechID: P2964 Researchers at the University of Pennsylvania have developed a new synthetic concept using TERMINI molecules (irreversible TERminator Multifunctional INItiator), in conjunction with living polymerization reactions that opens up unprecedented strategies for the design and synthesis of complex dendritic macromolecules from the largest and simplest class of commercial monomers. By enabling a living polymerization reaction to be interrupted and the same (or different) reaction to be subsequently started in more than one direction, the TERMINI molecule serves as a branchin...

Methods of Treating Cancerous Cells with Anti-Receptors Antibodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:14 GMT

TechID: D683 Currently, tumor treatments are primarily based on the cytotoxic effects of drugs and radiological therapy. Despite the fact that these treatments offer some remission, they unfortunately have side effects, killing not only tumor cells but also normal non-tumorous cells. There is a great need to find mammalian tumor treatments which target and specifically kill tumor cells with minimal effect on normal cells. With the advent of molecular genetic studies of cancer, it has been shown that oncogenes may play a role in the transformation of cells from their normal status ...

A Mouse Model for Hyperinsulinemic Hypoglycemia

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:14 GMT

TechID: N2549 Persistent Hyperinsulinemic Hypoglycemia of Infancy (PHHI), also known as familial hyperinsulinism, affects newborns and infants with a frequency of 1 case for every 40,000 or 50,000 newborns. Genetic variability accounts for Saudi Arabian estimates of 1:2500 births and a 1:3200 frequency in Finland. HI is responsible for 60% of the persistent hypoglycemia in infancy, with symptoms including growth deficiencies, convulsions, and brain damage in cases of misdiagnosis. This disease is characterized...

Nanotubes and Nanofilaments Dispersion in Thermoplastics by Coagulation Methods

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:13 GMT

TechID: P2999 There have been numerous attempts to disperse carbon nanotubes in thermoplastic polymers. Previous solvent and mixing-based approaches have shown some success, but these methods are not ideal means of obtaining versatile polymers with uniform nanotube dispersion. While in situ polymerization (O2802) has shown some improvement, there is still a need for better dispersion before these potentially valuable composite materials are used. Researchers at the University of Pennsylvania have now established a new method for manufacture of polymer-nanotube composites tha...

Flammability Properties of Poly(methyl methacrylate)-Single Walled Carbon Nanotube Nanocomposites

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:09 GMT

TechID: P3204 There is great interest in using nanoscale additives for making polymer nanocomposites with improved flammability properties in an effort to provide an alternative to conventional flame retardant (FR) materials. Research to date has centered on using clay, nanoscale silica and multi-walled carbon nanotubes (MWNT) combined with various polymers to achieve this goal. Researchers at the University of Pennsylvania recently demonstrated impressive flame retarding capabilities of SWNT/Poly[methyl methacrylate] (PMMA) nanocomposites produced using a proprietary method (P2999...

Adenoviral Vector Over-expressing Heat Shock Protein-70 (HSP-70) for the Treatment of Lung Injury and the Acute Respiratory Distress Syndrome (ARDS)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:07 GMT

TechID: N2433 The Acute Respiratory Distress Syndrome (ARDS) is a life threatening complication of a number of medical conditions. Worldwide, there are approximately 300-500,000 cases of ARDS yearly with a fatality rate of 30-50%. This disorder commonly occurs after severe trauma, intra-abdominal catastrophes, surgical procedures requiring massive transfusion, infections, etc. Pathophysiologically, ARDS is characterized by an accumulation of white blood cells in the alveolar septa, exudation of protein rich fluid into the alveolar air spaces, decreased pulmonary compliance, h...

Transcription Factor Synthesis in Dendrites: A Role in Nuclear Imprinting

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:37:06 GMT

TechID: J1651 The construction and maintenance of dendritic growth cones are influenced by a variety of factors, including some locally synthesized proteins necessary for dendritic outgrowth. Recently it was shown that mRNAs can be locally translated into proteins in individual dendrites and growth cones. Now specific local transcription factor mRNAs have also been recognized to modulate nuclear gene transcription in response to trophic or other cues. This “nuclear imprinting” permits distal dendritic signals ...

Nanoparticle and virus protection from immune cell uptake

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:59 GMT

TechID: V5072 For gene therapy, cancer treatment and other therapeutic applications, there is a need for drug/agent carriers that have a long circulation time in the body and can penetrate cell membranes. Current choices are limited to viruses and/or liposomes (vesicles comprised of natural phospholipids). However, most viruses cause side effects while liposomes are inherently leaky and have a short circulation time. More than a decade of research and collaboration among numerous researchers at the University of Pennsylvania has led to the discovery of polymersomes, a new type o...

Treatment of Human Inflammatory Diseases Using c-Rel Inhibitors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:57 GMT

TechID: U4676 The Rel/NF-?B family of transcription factors represents one of the most attractive targets for anti¬inflammatory therapy. Because Rel/NF-?B directly controls the expression of multiple inflammatory genes, its blockade is more effective for controlling inflammation than blocking one or a few downstream inflammatory genes or proteins. The first generation Rel/NF-?B drugs that block the entire Rel/NF-?B family have already been tested in both humans and animals. These include proteasome inhibitors (e.g., the FDA-approved PS-341), Rel/NF-?B decoy oligodeoxynucleotides an...

Expression System for Toxic Proteins for Gene Delivery

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:55 GMT

TechID: K1878 Serious problems have plagued the development of a cloning vector for toxic genes in bacteria. Sub-cloning of envelope genes has been extremely difficult using both single and double enzyme digestion methods. Traditional use of bacteria for cloning has been unsuccessful in this case because envelope proteins are toxic to bacteria and the Pcmv promoter has shown to be “leaky.” Researchers at the University of Pennsylvania have overcome these problems and developed an effective system for expressing toxic proteins for gene delivery. Applications Advan...

scFv thrombomodulin subdomain materials targeted to pulmonary endothelium

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:51 GMT

TechID: V4977 This invention provides compositions and methods of use that enable thrombomodulin to be used for its therapeutic advantages without incurring the undesirable side effects. Thrombomodulin is a key component of thrombotic and inflammatory processes. Thrombomodulin is an integral membrane protein expressed on the surface of endothelial cells and exerts direct effects in the endothelium, such as binding to a pro-inflammatory mediator that contributes to lethality in sepsis and acute lung injury. Thrombomodulin has both anti-thrombotic and anti-inflammatory effects; howeve...

Methods of Inhibiting Restenosis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:47 GMT

TechID: E964 Atherosclerosis is responsible for more deathes than any other disease in the United States. It is largely responsible for coronary artery disease, aortic aneurism and arterial disease of the lower extremities. This disorder involves thickening and hardening of wall portions of the larger arteries in mammals. These stenotic regions are characterized by a build up of fatty acid streaks, fibrous plaques and other lesions which result in a narrowing of the vessel and blood flow restrictions. Angioplasty has been widely used to treat atherosclerosis. Although results obtained by ...

iated Immunity with Listeriolysin (LLO)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:41 GMT

TechID: M2244 Cancers are characterized by the uncontrollable growth, proliferation, and spread (metastasis) of abnormal cells. Of the several treatments available to cancer patients, immunotherapy, which involves stimulation of the patient’s own immune system against cancer cells, is one of the most promising. Antigen vaccines stimulate the immune system by presenting a greater concentration of individual antigens tumor-specific to the immune system, rather than using whole tumor cells that contain many thousands of anti...

Design, Synthesis, and Evaluation of a Pyrrolinone-Based Matrix Metalloprotease Inhibitor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:38 GMT

TechID: N2410 Matrix Metalloproteinases (MMPs) are enzymes that are thought to play key roles under a variety of conditions, including cancer, arthritis, and wound repair. Compounds that modulate their activities are an active area of research, and a number of peptide-based inhibitors have been identified. Difficult-to-deliver peptides are not easily utilized therapeutically. Using patented technology, University of Pennsylvania researchers have successfully created a pyrrolinone-based peptidomimetic compound modeled on a known peptide-based MMP inhibitor, and dem...

A Drug Assay based on the protection from or Reversal of the Fragmentation of the Golgi Apparatus of Motor Neurons in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS, or Lou Gehrigs Disease)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:33 GMT

TechID: I1359 Amyotrophic lateral sclerosis (ALS) is a fatal degenerative disease, the classical sporadic form of which is characterized by the involvement of both upper and lower motor neurons and pyramidal tract degeneration. Recent studies indicate that the size of the Golgi apparatus is a reliable index of neuronal activity, and fragmentation of the Golgi apparatus is observed in Alzheimer’s disease (AD) and ALS. In order to assess the possible therapeutic effects of new treatments it is important to develop appropriate assays. Transgenic mice expressing a mutant form of superoxide ...

Peptide Analogs, Peptidomimetics, and Other Small Molecules Useful for Inhibiting the Activity of Ribonucleotide Reductase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:29 GMT

TechID: K1767 There are currently many disorders related to DNA replication, including tumor growth and metastasis and viral infections. The enzyme Ribonucleotide Reductase (RR) is an integral component of DNA replication and regulation. As such, an RR inhibitor would be clinically useful. While there are currently two types of RR inhibitors, neither is selective for a particular RR enzyme, thus causing widespread deleterious effects. Researchers at the University of Pennsylvania have developed a technology that permits the design, evaluation and preparation of select...

Gene for the Vacuolar Accumlation of Glutahione-Xenobiotic Conjugates by Plants

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:28 GMT

TechID: I1425 Animal and plant cells can eliminate a broad range of lipophilic toxins from the cytosol after their conjugation with glutathione (GSH). This process is mediated by a GS-X pump, which is a novel MgATP-dependent transporter. The detoxification of xenobiotics entails their oxidation, reduction or hydroxylation, conjugation with GSH and elimination by the GS-X pump. In plants, the GS-X pump is able to remove a number of xenobiotics, including herbicides. University of Pennsylvania scientists have identified genes which encode the GS-X pump and increase the capacity for v...

Mass Spectrometer Probe for Measurement of Gas Tensions

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:28 GMT

TechID: G1104 About 21,200 out of 53,000 of patients with Acute Respiratory Distress Syndrome (ARDS), and 200,000 out of 600,000 of patients with Pulmonary Embolism (PE) die each year in the US alone. This high mortality would be significantly reduced if early diagnosis of PE and a method to optimize mechanical ventilation in ARDS were available; however, no accurate and economical tool for these purposes exists today. Researchers at the University of Pennsylvania have developed an automated device, called MIGET by MMIMS, that measures ventilation/perfusion (V/Q) ratios of the pa...

Data Base Access System with Optimizable Expression

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:26 GMT

TechID: K1852 Relational databases provide convenient features for accessing large amounts of data, but they require prior knowledge of the type of data to be stored and have difficulty accounting for additional data and their relationships to existing data. On the other hand, semi-structured databases do not require such prior knowledge, but they exhibit shortcomings in efficient data access. In a joint effort with AT&T, Univ of Pennsylvania faculty have designed a database access system that restructures a use...

Role of N-Linked Glycosylation in the Secretion and Activity of Endothelial Lipase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:24 GMT

TechID: Q3450 EL is a glycosylated protein and human EL has five potential N-linked glycosylation sites. Using site directed mutagenesis, researchers at Penn hae generated mutant constructs of each glycosylation site in LPL and HL and have analyzed these mutants for their effects on secretion and activity of EL (Miller et al, J Lipid Res, 45, 2080-2087, 2004). These studies revealed that EL is a glycosylated protein and that lack of glycosylation at 4 out of 5 sites has different effects on the secretion and specific activity of EL. Applications Can be used as a research tool ...

Pseudotyping Lentivirus Vectors with Ebola Virus Capsids

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:24 GMT

TechID: M2305 Researchers at the University of Pennsylvania have developed a powerful recombinant transfer virus, in which a lentiviral minigene is packaged in a heterologous envelope comprising the binding domain of a filovirus envelope protein. Specifically, the filovirus is ebola, notorious for its ability to infect mammalian lung cells. Although the applications of such a transfer virus are broad, the possibility of a HIV vaccine is particularly promising. The ebola virus shell may be very effective for delivery of specific HIV genes, such as gag, nef, pol, to lung c...

Tyrosine Kinase Inhibitors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:23 GMT

TechID: L1919 p185c-neu, also known as Nneu, c-neu, p185, c-erb2, and HER-2/neu, identifies the normal neu protein. Acting synergistically with the epidermal growth factor receptor (EGFR), p185 has been found to play an important role in neoplastic transformation. Cell lines that simultaneously overexpress p185c-neu and the EGFR were determined to be transformed, based on refractivity of cells, focus formation, and tumorigenicity in nude mouse models. Although it has been shown that EGFR stimulates increased tyrosine phosphorylation of p185c-neu, the mechanism by ...

Affinity Chromatography Using Dried Calcium Aliginate-Magnetically Separation Media in a Magnetically Stabilized Fludized Bed

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:22 GMT

TechID: D705 Obtaining a purified product from a batch chromatographic separation demands that the feed be injected into the system for only a fraction of the total running time. This discontinuous introduction of feed leads to inefficient and labor intensive systems. Continuous sample injection and product withdrawal addresses the efficiency problem of batch operations but raises issued of expensive moving seals and the risk of contamination is high when the feed stream contains biological materials. A magnetically stabilized fluidized bed (MSFB) has many unique proper...

A Treatment for Cancers Expressing Chimeric NPM/ALK Tyrosine Kinase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:21 GMT

TechID: U4592 Anaplastic Large Cell Lymphoma, a type of Non-Hodgkin’s T cell lymphomas, is a highly malignant disease in which chromosomal translocation leads to the expression of a fusion protein, NPM (Nucleophosmin)/ALK(Anaplastic Lymphoma Kinase) Tyrosine Kinase (NPM/ALK). Cancer cells that are NPM/ALK+ve have a selective loss of STAT5A, which is an important tumor suppressor protein and a reciprocal inhibitor of NPM/ALK. STAT5a gene is intact in the cancer cells at the DNA level, but it is highly methylated and does not express STAT5A protein. DNA methyltransferase...

Surgical Method and Apparatus for Augmenting the Efficiency of Gene Delivery to the Heart

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:20 GMT

TechID: K1673 The invention is a method and a novel apparatus used to facilitate somatic transfer of genetic material to adult myocardium via the coronary vasculature while minimizing the invasiveness of the procedure. The inventors have optimized gene therapy to the myocardium by creating a device and procedure that establishes in vivo an isolated cardiac circulation. Applications Advantages

A2A9/6 Clone

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:16 GMT

TechID: P2988 One of the initial steps of clot formation is the crosslinking of platelets by fibrinogen through the aIIb/ßIIIa receptor. Researchers at the University of Pennsylvania have developed a monoclonal mouse antibody (IgG2a) to the human IIb/IIIa receptor. This antibody binds with high affinity, reacts with cells expressing IIb/IIIa in FACS assays, and will immunoprecipitate the IIb/IIIa complex from cell lysates. In addition, this antibody has been shown to inhibit platelet aggregation induced by a variety of agonists. Therefore, this monoclonal antibody ...

Mouse Model of Barrett's Esophagus

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:14 GMT

TechID: N2610 Over the past two decades, esophageal adenocarcinoma has been the most rapidly increasing cancer in the United States. A major risk factor for this cancer is a condition known as Barrett’s esophagus, in which the cells lining the esophagus acquire a similar morphology and function to those cells in the intestine. Also known as intestinal metaplasia, this change develops in response to chronic irritation such as acid reflux. Statistics indicate that over 20 million people in US suffer from chronic acid reflux, with 10-20% developing ...

Receptor-Binding Mutants of Influenza Virus HA for Use in Targeted Gene Delivery

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:13 GMT

TechID: K1715 Issued Claim 1 of 30: A lipid-containing vector which fuses to a cell membrane, said vector comprising a mutant hemagglutinin, wherein said hemagglutinin comprises a mutation in the receptor binding pocket of said hemagglutinin, wherein said mutation substantially abrogates binding of said hemagglutinin to a sialic acid containing receptor, and further wherein said mutation does not affect the fusogenic capacity of said hemagglutinin. Patent: US 6,416,997 Applications Infectious diseases Mode of delivering gene therapy Advantages Specific targeting ...

Minimum Power Pulse Synthesis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:09 GMT

TechID: P3041 Penn researchers have developed a method for RF-pulse sequence design for selective excitation of ensembles of two state quantum systems that allows for careful control of both the magnitude and the phase of the excitation profile. In principle this approach produces RF-pulse sequences of infinite duration, but, empirically they can be truncated without significantly altering the system response. Their approach is based upon an interpretation of the pulse synthesis problem as an inverse scattering problem for the 2 × 2-AKNS system. This is explained in the JMR paper,...

Peptide Inhibitors of a Human gpIIb/IIIa-Specific Platelet Antibody (Combined into O2912)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:09 GMT

TechID: P2967 Researchers at the University of Pennsylvania have developed human monoclonal antibodies which bind with high affinity to platelet glycoprotein IIb/IIIa. The glycoprotein IIb/IIIa epitope to which the antibodies bind is located in the hinge region of the molecule. Therefore, following treatment with the antibodies, GPIIb/IIIa cannot extend to interact with von Willebrand factor and the platelet remains inactivated. The duration of antibody activity is able to be controlled by the administration of peptides which specifically inhibit the GPIIb/IIIa an...

Structural Basis of Endothelial Lipase Tropism for HDL

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:06 GMT

TechID: Q3584 Lipoprotein Lipase (LPL) and Endothelial lipase (EL) are the most closely related enzymes among the members of triglyceride lipase gene family. They have distinct lipolytic properties as well as different preferences for specific types of lipoproteins. Investigators at Penn have generated LPL-EL and EL-LPL chimeric enzymes by exchanging their C-terminal domains and tested their ability to bind and hydrolyze native lipoproteins in vitro and in vivo (Broedl et al, FASEB J, Sep 28, 2004). Using these chimeras, they found that the C-terminal domain of EL determines the ab...

Emissive Polymerosomal Structures for In Vivo and In Vitro Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:05 GMT

TechID: P3164 See E960 parent docket. Applications Advantages

MX-POST Part of Speech Tagger

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:36:05 GMT

TechID: C.98-76 Many natural language tasks require the accurate assignment of Part-Of-Speech (POS) tags to previously unseen text. Researchers at the University of Pennsylvania have developed a Maximum-Entropy Part-of-Speech Tagger that assigns a part-of-speech label (tags) to each word of a previously unseen text with state-of-the-art accuracy of 96.6%. Furthermore, Penn tagger combines diverse forms of contextual information in a principled manner, and does not impose any distributional assumptions on the training data. It provides a better use of context, which improves the accuracy. ...

Development of a novel class of PI3K inhibitors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:57 GMT

TechID: U4769 Researchers at Penn have developed a class of organometallic rhuthenium scaffolds that can be selectively modified to inhibit the activity of several protein kinases such as GSK-3, PIM-1 and PI3-K implicated in diseases such as diabetes, alzheimers, bipolar disorder and even cancer. The in vitro potency of these compounds is several orders of magnitude better than the classic organic inhibitors, for example they demonstrate an IC50 in the picomolar range. The inhibitors have been demonstrated good solubility water and good stability in air. To our knowledge, no org...

Human Synovial Fibroblasts

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:56 GMT

TechID: M2340 Rheumatoid arthritis affects more than 2 million people in the United States and is two to three times more common in women than in men. It is characterized by hyperplasia of synovial membrane, which lines the joint cavity, and bone destruction. While the exact pathology of RA is not known, synoviocytes, the cells that comprise the synovium, play an important role in the excessive inflammation that leads to joint damage. Another contributing factor is the Fas protein found in synovial cell....

Controlled Release Polymersomes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:55 GMT

TechID: O2863 Researchers at the University of Pennsylvania have developed vesicles that are made from polymers called polymersomes. These vesicles are stable in the bloodstream and lack disadvantages common for liposomes, and can be advantageous for drug delivery applications. Polymersomes are large (˜100 nm) vesicles made from biodegradable, inert, FDA-approved polymers that feature enhanced durability, elasticity and resistance to physical and chemical stress in comparison with liposomes. An initial description of polymersomes can be found in Issue 284 (May 199...

A Method for Fabricating Integrated Nanostructures and Applications Thereof

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:53 GMT

TechID: Q3470 Pipettes can be found in every chemical, medical or biology laboratory, however with the advance of molecular biology, researchers require pipettes that were able to confine smaller and smaller amounts of specimen, not only for probing but also for injecting drugs, DNA etc. into cells - without damaging the cells. The tip of a glass pipette can be fabricated to have an inner diameter as small as several tens of nanometers; however there are considerable problems in terms of the processing precision and the operability of the pipette, particularly in terms of locatin...

Diacylglycerol Mimetics and Methods of Using the Same as Protein Kinase C Alpha Activators and Apoptosis Inducers

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:53 GMT

TechID: O2900 Diacylglycerol (DAG), a lipid second messenger generated by cellular phospholipases, activates many isozymes in the family of protein kinase C (PKC), a phospholipid-dependent serine/threonine-specific kinase that plays important roles in cell growth and differentiation. The activation of PKC by DAG mediates the actions of a variety of hormones, neurotransmitters, and other biological regulatory factors. In particular, PKC activated by DAG or its mimetics is shown to induce apoptosis. This leads to the hope that DAG mimetic compounds could be used as chemoth...

Constitutive Triple Response Gene and Mutations

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:52 GMT

TechID: F975 Ethylene, one of five well-established plant hormones, mediates fruit ripening, leaf abscission, and flower senescence. The production of ethylene is induced by stress conditions. Ethylene induces an effect on dark grown seedlings called the triple response, which may provide seedlings with additional strength to penetrate compact soils. University of Pennsylvania researchers have isolated a collection of mutants affected in this response, including the constitutive triple response (ctr) and the ethylene insensitive response (ein). The ctr mutan...

Recombinant Adenoviruses Encoding Trk Receptors and Neurotrophins

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:45 GMT

TechID: N2528 Neurotrophic factors are molecules that modulate the survival, growth, differentiation or regeneration of neurons, and become up or down regulated after modulate in a variety of neural insults. Through retro- and anterograde exchange of neurotropho between pre- and postsynaptic intners. Trk signaling through the family of tyrosine kinase receptors play a number o fkey roles in neuronal plasticity including the regulation of neurotransmitter release and synaptic stability. However, many biologigical roles hav...

Synthesis of (-)-Discodermolide

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:44 GMT

TechID: J1531 The current invention relates to lactone-containing compounds such as discodermolide, to compounds which mimic the chemical and/or biological activity thereof, and to methods and intermediates useful in their preparation. These compounds have been found to possess striking properties, for example, microtubule stabilizing properties superior to that of paclitaxel, potent inhibitors of cell proliferation and powerful immunosuppressive agents. The extreme scarcity from natural sources, unprecedented cytotoxicity, and structural complexity of these metabolites...

HSD Monoclonal Antibodies as Diagnostic Tools

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:43 GMT

TechID: L1959 The specific repertoire and concentration of steroid hormones in different tissues are responsible for their normal development and differentiation. The abnormal presence of these hormones, as well as the abnormal presence of the enzymes responsible for producing either the active or inactive forms of them, have been associated with a number of diseases. Along this line, hydroxysteroid dehydrogenases (HSDs) are enzymes responsible for the conversion of certain steroid hormones into their cognate, inactive metabolites. Abnormal expression of some HSD isoform...

Resistance-Switching Oxide Film Devices

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:41 GMT

TechID: S4065 Current non-volatile memory market, counts about a third of the entire $60B memory market, is dominated by flash memories. However, existing flash memory technology is facing challenges of unable to scale down to 22-nm, the cell size projected for year 2016 memory market. According to a paper published in Science (Vol 317 (2008) P1625), resistance-witching cell is one of the most promised contenders for replacing flash cells. Resistance-switching-type memories have been studied for many years, o...

Magnetic Resonance Imaging of Perfusion Using Spin Inversion of Arterial Water

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:39 GMT

TechID: D810 Tissue perfusion is important to the assessment of organ functionality. A number of techniques, such as tracers, are currently used to measure tissue perfusion. However, these methods are insufficient. Furthermore, tracers, such as Xenon, and diffusible tracers may require invasive procedures such as arterial blood sampling or yield variable results. University of Pennsylvania researchers have developed a novel method of analyzing tissue perfusion via MR imaging. Specifically, images are acquired when the fluid under examination is exposed to electromagnetic energy. ...

K19 Lac Z and GI/Pancreas Studies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:34 GMT

TechID: P3052 Keratins are intermediate filaments that are critical in cytoskeletal organization. Keratin 19 (K19) has unique structural properties and developmental and spatial expression patterns. Mutations in keratins are associated with disease states based on transgenic and knockout mouse models and human disorders (Science, 279, 514-519, 1998). The researchers at the University of Pennsylvania have generated and characterized transgenic mice using a K19 promoter fragment linked to ß-gal reporter gene. (Gastroenterology, 120, 1720-1728, 2001). The expression of this reporter gene was...

Human Tissue Factor Related DNA Segments Polypeptides and Antibodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:26 GMT

TechID: F1018 The clotting of blood involves a serial cascade of reactions mediated by a group of cellular and plasma proteins known as coagulation factors. Initiation of this cascade can occur when the cellular receptor tissue factor (TF) binds coagulation factor VII or its derivative, factor VIIa, to form a catalytically active complex. Although TF is not normally expressed on the surface of vasculature cells, the presence of infectious agents can induce monocytes within the vasculature to express it. The presence of TF on some extravascular cells may also result in clot formatio...

Detection of Breast Microcalcifications by Acoustic Ultrasound Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:21 GMT

TechID: I1362 Acoustic ultrasound imaging (AUI) uses low frequency acoustic signals together with ultrasound imaging to detect microcalcifications in tissue such as breast tissue. This invention provides a screening procedure complimentary to mammography which will substantially reduce the number of unnecessary biopsies. AUI involves exciting microcalcifications by external low frequency acoustic vibrations and then imaging the effect of those acoustic vibrations in the ultrasound signal. The acoustic frequency can be tuned to selectively image aspects of the microcalcificati...

Phorboxazole Compounds as Anti-Cancer Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:16 GMT

TechID: R3855 Produced by the Indian Ocean sponge Phorbas sp., the phorboxazoles, described by Searle and Molinski first in 1995, proved to be extremely potent tumor cell-growth inhibitors. When tested against the National Cancer Institute panel of 60 tumor cell lines, the mean GI50 values for 33 and 34 were 1.3 nM. In human colon tumor cells (HCT-15), phorboxazole A displayed selective inhibition with GI50 values as low as 0.426 nM, and as a result phorboxazole A has been selected by the National Cancer Institute for in vivo trials. These remarkable r...

A Murine Model for the Study of Left Ventricular Tissue Engineering

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:09 GMT

TechID: O2667 Congestive heart failure affects millions of people worldwide, and results in more than $30 billion annually in the US in treatment and hospitalization costs. The heart transplant needs of more than 300,000 people annually cannot be currently met by the few thousand suitable donor hearts that become available each year, and current drug and surgical therapies do little to extend the lifespan or quality of life for critically ill patients. New technology developed by researchers at the University of Pennsylvania may allow the replacement of diseased heart t...

Dopamine D-3 and Serotonin (5-HT1A) Receptor Ligands and Imaging Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:08 GMT

TechID: F994 University of Pennsylvania researchers have developed a series of tetralin derivatives (i.e., PIPAT) that selectively and strongly bind to specific central nervous system receptors, such as the dopamine-d3 receptor and the serotonin-5-HT receptor. These PIPAT derivatives can be radiolabeled with iodine and then used as diagnostic agents in SPECT or PET imaging. The compounds may be used as diagnostic brain imaging agents or they may be useful in the identification and evaluation of therapeutic agents that act at the serotonin and dopamine recept...

An Actuation System and Method for Pouch-Based Lab on a Chip

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:06 GMT

TechID: T4403 Lab-on-a-chip (LoC) technology is an emerging technology that enables the construction of discovery platforms for combinatorial biology and chemistry for drug and disease screening and for materials synthesis. It also facilitates diagnostic and characterization systems for, among other things, monitoring the quality of the water supply, detecting diseases at the point of care, and studying microbial diversity. The University of Pennsylvania (Penn) is one of the research leaders in LoC based diagnostic systems, Penn owns a comprehensive portfolio of in this area, in...

Magnetically Doped Ruthenates with Large Magnetoresistance

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:06 GMT

TechID: O2773 The multi-billion dollar market of portable electronics (cell phones, players, PDAs) is largely responsible for the astonishing growth of the microelectronics industry. Continuous demand for increased functionality, better performance, smaller power consumption and lower cost of electronic devices has been managed by constant miniaturization of its feature sizes. However, at the present stage the manufacturers are approaching the physical limits of currently used silicon. This is particularly important for applications involving a non-volatile memory. For example, exi...

Lithium Inhibition of GSK-3Alpha Reduces the Amyloid Production and Tau Phosphorylation Associated with Alzheimer's Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:05 GMT

TechID: O2720 Alzheimer’s disease (AD) is associated with aberrant processing of the amyloid precursor protein (APP) and increased levels of beta-amyloid peptides. Lithium inhibits APP processing by targeting glycogen synthase kinase-3 (GSK-3) ability to promote the gamma-secretase function of the presenilin proteins. Researchers at the University of Pennsylvania have identified the component of GSK-3 that promotes the processing of APP. Selective inhibition of this enzyme subunit, either pharmacologically or by blocking its expression, reduces the production of ...

A Novel 100 KD Protein Detected by Antibodies that Recognize a New Plaque Type in Alzheimer's Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:05 GMT

TechID: J1570 Alzheimer’s disease is characterized by senile plaques, neurofibrillary tangles (NFTs) and related neurofibrillary lesions. To further characterize these abnormalities, researchers at the University of Pennsylvania raised monoclonal antibodies against extracts of these lesions, which were used as immunogens. Of the antibodies generated, four were further characterized because of their unexpected immunoreactivity, with regard to NFTs or the hyperphosphorylated tau protein which comprises NFTs. However, these antibodies did react with a novel 100 KD protein a...

Inhibition of Histone Deactylase Activity as Treatment and Preventive Therapy for Ischemic Injury/Inhibition of DNA Methyl Transferase Activity as Treatments and Preventive Therapy for Ischemic Injury

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:04 GMT

TechID: R3701 Myocardial infarction is a common clinical entity, and the majority of related morbidity and mortality results from congestive heart failure. This resultant heart failure is due to extensive myocardial damage caused by ischemic tissue injury. Current therapies attempt to limit ischemic injury by achieving rapid patency of infarct-related vessels or by providing glucose and insulin to ischemic tissues. Targeted approaches, however, that take into account epigenetic modifications of DNA and histone proteins have not been explored. Researchers at the U...

Stabilized Enzyme/Inhibitor Complex

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:02 GMT

TechID: F1065 Serine protease inhibitors (serpins) are important regulators of a variety of biological processes including inflammation, fertilization, tumor migration, neurotropism, and heat shock. Defects in serpins are associated with lung, liver and blood coagulation diseases such as emphysema, liver cirrhosis, thrombosis, and pulmonary embolism. University of Pennsylvania researchers have provided a method of producing a serine protease inhibitor with an altered sequence to effectively modulate serine protease activity. A method to produce serine protease inh...

Synthesis of Functional Human Hemoglobin

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:35:01 GMT

TechID: D703 One out of every three individuals will at some point not have enough blood to sustain his or her life. This gives rise to a need for blood every three seconds; however, only five percent of the American population donates blood (American Blood Centers). Thus, there is a tremendous need for the development of artificial blood substitutes that can function to transport and deliver oxygen to the body’s tissues. Researchers at the University of Pennsylvania have developed a method to synthesize functional human hemoglobin in erythroid tissues of transgenic n...

Novel DNA Computer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:56 GMT

TechID: I1392 Penn researchers have developed a DNA procedure for solving a difficult problem in the mathematics of linear algebra, the calculation of the determinant of sparse matricies. Using small segments of DNA to represent each element in a matrix, this procedure leads to the construction of larger DNA molecules which represent all of the non-zero terms in the determinant of the sparse matrix. Because of the large number of potential terms in the determinant of sizeable matricies, this problem is difficult to solve by standard computer techniques.

A Rapid Method of Detecting Epitopes on Molecules

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:55 GMT

TechID: M2230 Protein expression, levels and post-translational modifications largely determine the phenotype of a cell. For example, liver cells and white blood cells contain the same basic genetic information in their DNA, but their different physiological roles in the body are based on the differences in how proteins are transcribed, synthesized and modified. Moreover, pathological states in cells, such as cancerous transformation, are reflected in the cell's protein phenotype. In addition, the protein phenotype also changes with time, both on short time scales and as organisms ...

Method for diagnosing lung cancers using gene expression profiles in peripheral blood mononuclear cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:53 GMT

TechID: V4888 Researchers at The University of Pennsylvania and the Wistar Institute have identified a group of genes in peripheral blood cells that are biomarkers of early stage lung cancer, which can be used to develop a screening, diagnostic, or monitoring assay for lung cancer. The methods and compositions provided describe a simple, cost effective and non-invasive blood test using gene expression profiling that could be used in the initial diagnosis of lung cancer or to differentiate malignant from benign disease. While early detection an...

Anti-CD4 #19; Anti-CD4 #21

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:50 GMT

TechID: P2985 Researchers at the University of Pennsylvania have developed two novel anti-CD4 monoclonal antibodies: clones #19 (IgG2a) and #21 (IgG1). Both antibodies were made from mice immunized with human Sup-T1 cells expressing CD4 and were subsequently screened for their ability to inhibit SIV fusion on a CD4 positive cell line. Clone #19 was found to be a competitive inhibitor of the Becton Dickinson antibody Leu3a and clone #21 is a partial inhibitor. Both monoclonal antibodies also recognize surface CD4 in FACS assays and fixed CD4 in tissue sections. ...

Digital Topological Analysis of the Trabecular Bone Structure

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:49 GMT

TechID: N2493 Evaluation of the mechanical strength of trabecular bone (TB), dependent upon material, scale, topology, mass density, architecture, and disease processes, can determine the onset of fracture due to osteoprosis or metabolic bone disorders. Unfortunately, current methods of diagnostic screening based on dual-energy X-ray absorptiometry (DEXA) which measures bone mineral density (BMD) does not distinguish between trabecular and cortical bone, nor does it address the structure of the bone. Currently, no existing method provides a reliable and efficient method to qu...

Pyrolinone-Based Compounds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:46 GMT

TechID: F1009 Peptides are implicated in a wide variety of physiological and pharmacological processes in humans. University of Pennsylvania researchers have designed peptide mimics that are not susceptible to cleavage and inactivation by peptidases yet maintain certain biological, chemical, and physical properties of a targeted peptide, especially b-strand and b-pleated sheet conformations. This new class of compounds contains linked sequences of pyrrolinone units in place of peptide amino acids mimicking the b-strand conformations of a number of naturally occurring pe...

Improved Electro-chemical Sensors and Methods for Their Use

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:45 GMT

TechID: D672 The detection of elemental and molecular species via electrochemical sensors is integral to numerous industrial operations. However, the harsh environmental conditions (i.e. highly corrosive, oxidative, etc.) such as those experienced in metal purification and processing, render electrochemical sensors inoperative. In these situations, electrochemical sensors have shown high failure rates, poor reproducibility, low sensitivity, etc. In response to this problem, University of Pennsylvania researchers Dr. Qingguo Liu, Dr. Yuhua Tang, and Dr. Wayne Worrell, have develo...

An Electronic Phantom Source for X-Rays Cameras

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:42 GMT

TechID: F1050 The University of Pennsylvania currently holds a patent for an electronic phantom source for PET and SPECT cameras. The inventor has developed a source which emits a narrow beam of mono-energetic slow-positrons. The beam spot size at the screen, placed in the camera’s field of view, is approximately 1mm, and the spot position may be computer controlled to simulate any phantom. The current problem of coregistration of MRI and PET is solved by using the MRI data to program the electronic phantom. The source consists of a thin walled inner cylinde...

Novel Compositions for the Treatment of Heart Failure

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:37 GMT

TechID: M2365 Congestive Heart Failure (CHF), in which the pump action of the heart is compromised, can arise from a number of conditions, such as loss of muscle function from infarction or infection, or excessive systemic blood pressure. Effective treatment of Congestive Heart Failure is aimed at increasing cardiac contractility, cardiac diastolic relaxation, cellular contraction, and vasodilation (widening of the lumen of blood vesels). Positive Inotropic agents increase cardiac muscle contractility in treatment of conge...

Cellular Proteins which Mediate Herpesvirus Entry

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:35 GMT

TechID: K1884 Statistically, one out of three Americans carry the HSV-1 or HSV-2 in the dormant state, ready to be activated when the host is stressed or immunocompromised. Currently there are no fully effective treatments for a herpesvirus infection in humans. The identification of compounds that inhibit entry of the herpesvirus into cells would greatly facilitate treatment of herpesvirus infection in humans. The present invention is based upon the discovery of two cellular proteins which mediate entry of HSV-1 and/or HSV-2 and other herpesviruses into cells. The...

Hydrolytically degradable polysaccharide hydrogels

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:29 GMT

TechID: V4899 Hydrogels have gained tremendous momentum in cell encapsulation and in controllably releasing therapeutic agents disposed within. Gels made up of polysaccharides, which are biological polymers that are found in numerous types of tissues and organisms and are capable of being crosslinked into hydrogels that encapsulate active agents (e.g., growth factors and other macromolecules) or cells for release or incubation by way of the hydrogel being degraded to liberate the encapsulated agent or cell. These hydrogels, however, typically degrade only in the presence of enzym...

CMV Adenoviruses for Screening of Potential Anti-Obesity Compounds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:21 GMT

TechID: R3889 See Nature 428, 569 - 574 (01 April 2004) : " AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus " To determine whether modulation of hypothalamic AMPK activity alters food intake and body weight, our researchers expressed constitutively active (CA, H150R mutation in the 1 subunit of AMPK) and dominant negative (DN) 1 (D157A)13 and 2 (K45R) subunits of AMPK in the medial hypothalamus using recombinant adenoviruses. The recombinant adenoviruses expressed AMPK subunit messenger RNA primarily in arcuate hypothalamus, vent...

Biofeedback Game Controller for Pain Management

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:19 GMT

TechID: M2227 Biofeedback is a useful technique in the management of pain. As an alternative to medications, honing Non-Pharmacological this technique allows appropriate patients to learn how to control physiologic responses that are normally not under voluntary control. It is clearly mediated by a different mechanism than diaphragmatic breathing, progressive muscle relaxation, and guided imagery, but probably influences the same underlying pain pathways. The biofeedback equipment package consists of a desktop computer, encoder box that translates physiological information to...

Metal Mediated Cross-Coupling with Ring Metalated Porphyrins

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:18 GMT

TechID: F1053 See E960 parent docket. Applications Advantages

Novel Pyrrolinone-Based Compounds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:13 GMT

TechID: H1278 Peptides are implicated in a wide variety of physiological and pharmacological processes in humans. University of Pennsylvania researchers have designed peptide mimics that are not susceptible to cleavage and inactivation by peptidases yet maintain certain biological, chemical, and physical properties of a targeted peptide, especially b-strand and b-pleated sheet conformations. This new class of compounds contains linked sequences of pyrrolinone units in place of peptide amino acids mimicking the b-strand conformations of a number of naturally occurring pe...

Screening Vaccines and Immunization Process

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:12 GMT

TechID: G1118 Researchers have developed a new method of producing vaccines to any infectious organism that binds specifically to mammalian host cells, but in particular to viruses, including reoviruses and rotaviruses. Monoclonal antibodies are produced against the binding site on the infectious organism for the host cell and are screened for neutralizing ability. Next a second set of monoclonal antibodies directed against the first set of monoclonal antibodies is produced. These second antibodies are now able to bind specifically to the same site on the host cell as th...

A BLIS-ful Alternative to Antibiotics for the Treatment of Acne

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:07 GMT

TechID: S4013 BLIS is Bacteriocin Like Inhibitory Substance, a lantibiotic subtype. BLIS is ribosomally synthesized as a larger amino acid precursor peptide. In the investigations of various BLIS agents, a subset of S. salivarius demonstrated BLIS activity against p. acnes, for example. Even S. salivarius isolates that were resistant to acne antibiotics were able to produce BLIS with activity against P. acnes. Of 11 strains examined that inhibited p. acnes, 5 were unable to inhibit Group A Strep. Therefore, there appears to be a BLIS substance distinct from SAL A2 or B. Applic...

Xenografting of Testis Tissue

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:06 GMT

TechID: O2723 Males who undergo chemotherapy frequently lose their ability to father children as their spermatogonial stem cells are destroyed during treatment. Prior to treatment, men are able to bank sperm for future use, but immature boys do not have this option. Researchers at the University of Pennsylvania have developed technology in which immature testis tissue may be transplanted under the skin of a nude mouse, and the transplanted tissue grows and matures to produce fully differentiated sperm. The utility of the technique has been demonstrated using both ...

Murine Glomerular Endothelial, Mesangial, and Epithelial Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:03 GMT

TechID: O2748 Glomerular disease is a major cause of chronic renal failure, and glomerular injury occurs during the course of all forms of progressive renal disease. Glomerular endothelial, visceral epithelial and mesangial cells were derived from disease free, transgenic mice, where non-continuous expression of a temperature-sensitive SV-40 T antigen enabled rapid expansion of cells for study under physiologic conditions. Glomeruli were isolated from normal an...

A Novel Class of Positive Inotropic Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:34:01 GMT

TechID: H1210 Inotropic agents modulate cardiac muscle contractility. University of Pennsylvania researchers have developed a new class of inotropic agents that act as positive inotropic agents, profoundly increasing the contractility of cardiac muscle. These compounds are adenine nucleotide analogs. Preliminary data in isolated ventricular cardiac myocytes indicate that these adenine nucleotide analogs may act by a mechanism different from those described for any of the known inotropic agents, such as the beta-adrenergic agonists dobutamine and dopamine; the Na+/K+ATPa...

Cyclic Hexapeptides

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:58 GMT

TechID: I1409 Researchers at the University of Pennsylvania have designed molecular compounds, known as peptide analogs, which contain no peptide bonds but mimic or inhibit the chemical and/or biological activity of peptides. A number of hormones and neurotransmitters are controlled by receptor-mediated stimulation of one or more of a family of guanine nucleotide-binding regulatory proteins, known as G-proteins. The transmembrane signaling pathway used by G-protein-linked receptors represents one of the major mechanisms of signal transduction in cellular systems.&...

Computer Simulation of Biomembranes Using a Coarse Grain Model

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:51 GMT

TechID: O2880 Many key biological processes, including membrane fusion, membrane-protein interaction, and oxidative phosphorylation, occur at the membrane level. The concept of micron size domain formation in membrane (“rafts”) has been used to explain mechanisms involving lateral lipid organization. A broader understanding of the lipid interactions at a mesoscopic level is desirable and can reveal more details in the study of biomembranes. Atomic simulation techniques have been used to model phospholipid membranes. However, current algorithms and comp...

Nanoencapsulated Antioxidant Enzymes for the Treatment of Sub-Acute/Chronic Oxidative Stress

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:42 GMT

TechID: Q3349 Researchers at the University of Pennsylvania have designed a novel method to produce a biocompatible and biodegradable nanocarrier encapsulating active protein or enzymes resistant to protease degradation. This technology allows encapsulation of therapeutic doses of large hydrophilic proteins, such as enzymes, while retaining their activity for a time period optimized for the desired therapeutic time window. The nanocarriers are permeable to the enzymatic substrates, but not to macromolecular proteases, thus providing prolonged therapeutic effect.&nbs...

Electrochemical Devices Based on Single-Component Solid Oxide Bodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:41 GMT

TechID: D843 Solid oxide fuel cells (SOFC) hold some inherent advantages over other fuel cells such as molten carbonate, phosphoric acid (high temperature operation; corrosive) or membrane types. SOFC’s typically possess efficiencies approaching 80%. They require less energy input for specific power load. SOFC have suffered in the past from relatively low energy density and fabrication problems. For instance, the laminated structure of older SOFC’s often results in delamination after several use cycles; differential expansion of the laminate results in delam...

Compositions and Methods of Treating Tumors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:40 GMT

TechID: G1157 The Neu gene product p185 is a transmembrane glycoprotein with tyrosine kinase activity present in many tumors. It is homologous with the epidermal growth factor receptor (EGFR) and also dimerizes with EGFR, which potentiates its kinase activity. A mutation resulting in constitutive kinase activity has been implicated in several human adenocarcinomas, including gastric, lung, and pancreatic cancers. University of Pennsylvania researchers have developed a novel method to treat mammalian tumors which contain cells expressing the Neu gene product, p185, ...

Random shRNA Library

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:34 GMT

TechID: R3854 Dr Wilson and Dr Wang have created the first known library encoding short-hairpinloop RNAs (shRNAs) that are completely random at the nucleotide level. Other RNAi libraries limit discovery to sequences that target single genes and act through canonical RNAi mechanisms. The random nature of the Penn shRNA library is vitally important for the discovery of novel therapeutics and biologic tools because the most effective sequences will likely target multiple genes and/or act through one or more of the many alternative mechanisms that are only beginning to be elucidated. A...

AME Molecules that Bind p185 neu/her2 Sensitize Human Tumors to Radiotherapy and Convert Radioresistant ones to radiosensitive tumors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:33 GMT

TechID: K1866 The erbB family of receptor tyrosine kinases (RTK) is overexpressed or mutated in a large variety of human cancers. Homo- or heterodimerization of this family is induced by ligand binding to the RTKs. While these interactions are important for normal cell growth, alteration in the expression of erbB receptors occurs in numerous tumor types and plays an important role in the development and progression of the disease. Researchers at the University of Pennsylvania have developed therapeutic approaches to cancers in which the erbB genes are overexpresse...

A reagent for modulating the function of Hsp70/DnaK

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:31 GMT

TechID: V4885 -A novel small molecule has been identified that is capable of inhibiting or modulating the functions of the stress-inducible molecular chaperone HSP70 (human) and its bacterial orthologue DnaK (bacterial). This reagent is intended to be used alone or in combination with other reagents to effectively induce cell death in targeted tumor cells or in antimicrobial applications. The stress-inducible HSP70 protein generally is present at low levels in unstressed normal cells but exhibits elevated expression in tumor cells, and in response to factors such as ...

Novel MR Contrast in Biological Tissues (Combined into N2436)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:29 GMT

TechID: N2446 Current image contrasting methods which exploit T1 and T2 relaxation times, proton density, magnetization transfer effects, and endogenous contrast agents are inadequate for detecting and quantifying early changes in a number of pathologies. Proton T1rho imaging, therefore, is becoming increasingly exploited to probe macromolecular interactions in a number of tissue structures, including cartilage. However, the specificity of image contrast decreases when multiple structures contribute to the image contrast. Researchers at the University of Pennsylvania have cre...

The Vacuolar H+-pyrophosphatase: A Novel High Capacity Proton Pump

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:28 GMT

TechID: M2257 Plant cells contain alternative metabolic pathways that utilize nucleotides or inorganic pyrophosphate (PPi) as energy sources. This pattern of alternate proximate energy sources is exemplified by the presence of two parallel proton pumps in the vacuolar membrane. These are a multiple subunit vacuolar-type ATPase (V-ATPase) and a single subunit vacuolar-type pyrophosphatase (V-PPase). Both enzymes catalyze electrogenic proton translocation from the cytosol into the vacuolar lumen to establish a p...

New Method for Measurement of In Vivo iNOS Activity in Adherent Cultured Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:26 GMT

TechID: P2990 Background Nitric Oxide (NO) is a key physiological mediator, and the association of disordered NO generation with many pathological conditions has led to much interest in pharmacologically modulating NO levels. Understanding the mechanisms of NO production is a rapidly growing area of research in academic settings and pharmaceutical companies. In the human body NO is synthesized by three classes of enzymes called NO synthases (NOS), with inducible or inflammatory NOS (iNOS) being widely implemented in a variety of inflammatory processes. Several types of cells, inclu...

Mode of Action of Timolol in Medical Treatment of Glaucoma

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:22 GMT

TechID: L2070 Glaucoma is a major cause of blindness that is commonly treated medically by reducing the inflow of aqueous humor into the eye, thereby lowering intraocular pressure. Unfortunately, all current therapies have significant side effects and reduce patient compliance. Researchers at the Univeristy of Pennsylvania have recently developed a novel method of reducing intraocular pressure in individuals with glaucoma. This invention demonstrates that in contrast to previously believed c-AMP-mediated mechanism...

Vascular Pre-Conditioning Limits Subsequent Injury Response

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:19 GMT

TechID: I1418 Cardiovascular disease is the major cause of death in the United States today. In attempts to treat atherosclerotic lesions using current technology, there is invariably injury to the vessel wall whether due to the angioplasty balloon pressure or the handling and suturing of vein grafts. The uniform response to such injury is a gradual blood vessel wall thickening known as intimal hyperplasia. University of Pennsylvania researchers have developed a technique to limit the intimal hyperplastic response using endogenous protective proteins known to be expressed in the ves...

HNF3 Alpha Null Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:13 GMT

TechID: L2025 The HNF family of transcription factors is important for regulation of both islet hormone genes and genes involved in hepatic glucose production. Mice homozygous for a null mutation in the transcription factor HNF3alpha (HNF3alpha -/-) were recently created at the University of Pennsylvania. These homozygous mutant mice show severe growth retardation after birth and die in the postnatal period (day 12) from hypoglycemia, weakness, and dehydration. Inactivation of the HNF3alpha gene has been achieved via gene targeting. The mutation deletes the entire DNA-binding doma...

Fluctuation Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:13 GMT

TechID: I1475 Researchers at the University of Pennsylvania have invented a technique which uses visible light to quantify and image the flow of blood. The technique employs a source of low-power coherent light, and detects the speckle pattern of the diffusely scattered photons from the object being imaged. After a certain time moving particles, such as red blood cells, will be in positions that do not correlate with their original positions. The light that is scattered from these moving particles will therefore lose its correlation with previously scattered light...

Use of Cyclophilin B and Mutations Thereof As Modulators of Somatolactogenic Function

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:08 GMT

TechID: L1951 Prolactin (PRL) and growth hormone (GH) and are neuroendocrine hormones with pleiotropic functions that regulate human growth, immune response, and mammary differentiation, lactation, and neoplasia. These regulatory activities are mediated in part by GH's and PRL's respective cell surface receptors. The inventor’s earlier research suggested that these hormones act within the cell nucleus. The present invention elucidates the mechanism of somatolactogenic hormone retrotranslocation and direct action within the nucleus. More specifically, the inv...

Push-Pull Arylethynyl Porphyrins

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:07 GMT

TechID: H1320 Future generations of optoelectronic devices for telecommunications, information storage, optical switching, and signal processing are predicated to a large degree on the development of materials with large nonlinear optical (NLO) responses. Toward this end, considerable effort has been directed to elucidating the chromophore design elements that correlate most closely with large first-order hyperpolarizabilities. A NLO chromophore with high molecular first hyperpolarizability would be a desirable component of electric field poled-polymeric systems, thus s...

Controlled Release Worm Micelles

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:33:07 GMT

TechID: Q3518 For gene therapy, cancer treatment and other therapeutic applications, there is a need for drug/agent delivering carries that have a long circulation time (few days) in the body and are permeable to cell membranes. Current choices are viruses and/or liposomes (vesicles comprised of natural phospholipids). However, most viruses cause side effects while liposomes are weak, environmentally sensitive and have a short circulation time. Over ten years of research and collaboration among multiple researchers at the University of Pennsylvania have led to the discovery of ...

HNF38 Promoter Cdx2 Transgenic Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:59 GMT

TechID: M2331 Intestinal metaplasia is important to human disease because it is a precursor to many cancers, including adenocarcinoma of the esophagus and stomach. Also known as Barrett’s esophagus when involving this organ, intestinal metaplasia is the transformation of the differentiated mucosa of the stomach and esophagus to an intestinal phenotype with the expression of genes specific to the intestine. Researchers at the University of Pennsylvania have created a disease model whereby a genetically altered strain...

Methods and Devices for Running Reactions on a Target Plate for MALDI Mass Spectrometry (Combined into O2715)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:58 GMT

TechID: Q3325 Proteomics and high throughput drug screening involve the analysis of hundreds to millions of individual samples. Typical drug screening methods rely on optical signals such as fluorescence or luminescence to identify a hit or successful reaction, but there are inherent disadvantages to such an approach such as reduced sensitivity and the generation of expansive data sets when multiple signals are present in individual samples. As a result, “label free” screening methods, such as matrix assisted laser desorption ionization (MALDI), are actively being pursu...

Methods Using Cre-Lox for Production of Recombinant Adeno-Associated Viruses

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:57 GMT

TechID: J1650 Researchers at the University of Pennsylvania have developed a method for producing a rAAV. A first vector containing the cre transgene under regulatory control of sequences which express the gene is introduced into the host cell, followed by a second vector containing a spacer flanked by lox sites, which is 5' to the rep and cap genes, and finally, a third vector containing a therapeutic transgene flanked by AAV ITRs. These vectors may be plasmids or recombinant viruses. One of the vectors can be a recombinant adenovirus or herpesvirus, which can provide to the host cell th...

Cysteine Increase in Malignancy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:56 GMT

TechID: J1534 Low molecular weight thiol-containing compounds, such as cysteine, play a key role in protecting cells from ionizing radiation, other free-radical generating reactions, reactive oxygen species, and chemical toxins. The presence of cysteine in the tripeptide glutathione (GSH) provides a much greater reserve of intracellular thiols than free cysteine. While GSH’s protective role has been emphasized in multiple studies, cysteine’s neutral charge and smaller size enable its greater access to negatively cha...

A Causal Role for the SNF-1/AMPK-Related Kinase, Hunk, in Mammary Tumor Metastasis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:55 GMT

TechID: R3775 Tumor metastasis is a major factor affecting cancer-related morbidity and mortality. Genetic patterns have been identified for tumors with a propensity to metastasize. Identification and characterization of the genes associated with recurrent and metastatic cancer will lead to new drug targets and more effective treatments. Breast cancer is one of the most common malignancies in women. Breast cancer recurrence after a period of stable or no clinical evidence of disease remains the most common cause of mortality associated with breast cancer. Recur...

Akt1Null Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:54 GMT

TechID: O2680 The serine threonase kinase, Akt, also known as protein kinase B, is an important effector for phosphotidyl-inositol3-kinase signaling initiated by numerous growth factors and hormones. The isoform Akt1 has been implicated in skeletal muscle hypertrophy [Nature & Cell Biology, 3, 1014-1019, 2001] and MDM2 phosphorylation [Nature & Cell Biology, 3, 973-982, 2001]. Researchers at the University of Pennsylvania have developed an Akt1 knockout mouse [JBC, 276, 38349-38352, 2001]. These null Akt1 mice demonstrate defects in fetal and postnatal growth thr...

Pyrrolinone Based Protease Inhibitors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:47 GMT

TechID: H1215 Peptides are implicated in a wide variety of physiological and pharmacological processes in humans. University of Pennsylvania researchers have designed peptide mimics that are not susceptible to cleavage and inactivation by peptidases yet maintain certain biological, chemical, and physical properties of a targeted peptide, especially b-strand and b-pleated sheet conformations. This new class of compounds contains linked sequences of pyrrolinone units in place of peptide amino acids mimicking the b-strand conformations of a number of naturally occurring pe...

Virtual Bone Biopsy (VBB) System

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:44 GMT

TechID: O2705 University of Pennsylvania researchers have devised a virtual bone biopsy (VBB) data acquisition and analysis system that acquires MR microimages of distal radius or tibial trabecular bone to help evaluate bone diseases, such as osteoporosis and renal osteodystrophy. The system is divided into three components: acquisition hardware, acquisition software, and processing system. The 3D image of a region of trabecular bone is converted into a skeletonized surface representation and examined by digit...

Therapeutic Targets in Fragile X Disease (Combined into P3091)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:41 GMT

TechID: O2729 Fragile X syndrome (FXS) is the most common form of inherited mental retardation. FXS results from decreased expression of the fragile X mental retardation-1 (FMR1) gene. A single point mutation within the RNA binding domain, of the FMR1 gene product, is sufficient for inducing a severe form of the FXS symptoms in mice. It is believed that loss of FMRP function effects the translation of several mRNAs. By isolating mRNAs that bind specifically to FMRP, researchers at the University of Pennsylvania have identified several possible therapeutic targets f...

Nitric Oxide Production by Resorbing Human Osteoclast-like Cells from a CD34+ Pagetic Cell Line

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:40 GMT

TechID: K1869-X Multiple myeloma, Paget’s Disease, rheumatoid arthritis, and many other disorders are characterized by pathological osteoclast activity. Unfortunately, study of these disorders has been hindered by the lack of in vitro models. Researchers at the University of Pennsylvania have developed immortalized human cells which exhibit osteoclast activity. These cells were shown in vitro to excavate focal pits on a calcified substrate of a comparable area to those formed by avian osteoclasts. Therefore, this novel human osteoclast-like cell line may be ...

Scanning Impedance Microscopy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:38 GMT

TechID: N2478 Penn researchers have developed a novel and simple-to-assemble method and apparatus to measure local impedance in microcircuitry. Currently, there is no way to measure point-by-point, or segment-by-segment values for both the amplitude and phase angle of local impedance in microdevices such as integrated circuits, thick film circuits, electronic ceramic devices, and MEMS. Commercially available technology only allows for an estimate of the average value throughout the entire surface of the device, or large portions of it. Penn’s ...

Snail, An Inducer of Epithelial-to-Mesenchymal Transitions, Promotes Mammary Cancer Recurrence

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:31 GMT

TechID: R3752 Breast cancer is one of the most common malignancies in women. Breast cancer recurrence after a period of stable or no clinical evidence of disease remains the most common cause of mortality associated with breast cancer. Recurrence usually happens within 1-2 years after diagnosis; however recurrence following a 10-20 year period of dormancy is possible. Approximately 180,000 new cases of invasive breast cancer are diagnosed with 40,000 deaths in the US each year. There is a significant need to understand the mechanisms associated with recurrence ...

Tagged Human L1 Retrotransposons

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:28 GMT

TechID: O2714 Knock-out mice generated by random mutagenesis are essential tools for functional genomics to study gene functions in physiological and disease processes and identify new targets for therapy. Several methods, including chemical and x-ray mutagenesis and retrovirus gene trapping, are currently used to create mice carrying randomly mutated genes. However, chemical and x-ray mutagenesis require extensive efforts to map the mutations, while retrovirus gene trapping is not entirely random and require the creation of mutant mice from embryonic stem (ES) cells. Therefore, a f...

AMP Deaminase Activity Influences Clinical Outcome in Patients with Heart Failure

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:27 GMT

TechID: J1485 Congestive Heart failure (CHF) is characterized by diminished cardiac contractile function and decreased exercise tolerance. Even with recent advances in medical therapy, the rate of demise in patients is about 40% within one year of diagnosis and 70% after five years. In addition to age, decreased cardiac output, and high blood pressure, an important parameter of CHF survival is the individual genetic contribution. Studies have implicated mutations of the adenosine monophosphate deaminase 1 (AMPD1) gen...

Selective Inhibition of Cell Proliferation by VAV Antisense Oligonucleotides

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:24 GMT

TechID: G1110 Vav is a proto-oncogene expressed exclusively in hematopoietic cells of all lineages and stages of maturation. Vav likely serves as a transcriptional activator and/or element of the signal transduction cascade. Antisense oligonucleotides form stable complexes with the Vav transcript and display a striking differential toxicity to different hematological cell types. University of Pennsylvania researchers have developed a method to selectively inhibit proliferation of certain cells of hematopoietic origin usin...

Actinobacillus Actinomycetemcomitans Cytolethal Distending Toxin-- Immunoinhibitory Protein

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:23 GMT

TechID: S4205 Periodontitis is a common inflammatory condition affecting the supporting tissue of the teeth and is often caused by a persistent oral infection of facultative non motile gram negative bacterium [A. actinomycetemcomitans and related]. More than 30-50% of Americans are afflicted by periodontitis and many experience significant bone loss in and around the teeth. Although good oral hygiene combined with Chlorhexidine gluconate mouthwashes is the recommended ounce of prevention, many strains of bacterium are resistant to such treatments. Based on th...

New Vaccine to Prevent Infection with Streptococcus Pneumoniae

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:22 GMT

TechID: R3857 Pneumococcal pneumonia results in 500,000 cases and 40,000 deaths each year in the US. The technology comprises a set of attenuated strains of Streptococcus pneumoniae that are mutants with deletions for two virulence factors that alone or together would constitute strains capable of colonizing while not causing pathogenesis. Dr. Weiser’s group has achieved 100% protection against infection in mice with a single colonizing dose of a live, unencapsulated attenuated mutant without any need for further attenuation. The attenuated strains (or vehicle alone control) were ...

Rabbit Anti-Mouse Hif-1 Alpha Polyclonal Antibody

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:20 GMT

TechID: R3703 Hypoxia-inducible factor (Hif) is a heterodimeric transcription factor composed of a and ß subunits. It mediates cellular responses to low oxygen concentration by transcriptional activation of genes involved in angiogenesis and tumorogenesis, and so manipulation of the Hif pathway has potential therapeutic uses in cancer and ischemic heart disease, among others. Penn researchers have produced a polyclonal Ab against the Hif-1a subunit which can detect the presence of this subunit at very low levels on Western blots, and without cross-reactivity towards Hif-2.a Ap...

Production of Recombinant Human Tryptase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:19 GMT

TechID: N2574 Human tryptase is a proteolytic enzyme produced by human mast cells that populate connective tissue and epithelial linings such as the skin, respiratory and gastrointestinal tracts, and small blood vessel perivascular tissue. The enzyme may contribute to a number of biological and pathological processes including inflammation, tissue damage, asthma, arthritis, and blister formation in skin diseases. Production of tryptase inhibitors may have a number of useful applications in diseases with inflammatory ...

Motor Neuron Degeneration is Causally Related to Factors that Regulate the Stability of Neurofilament mRNA

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:17 GMT

TechID: L1915 Motor neuron diseases, such as ALS, cause the relentless progression of muscle weakness, paralysis, and death in affected individuals. These diseases are due to degeneration and loss of motor neurons. Transgenic models of motor neuron degeneration in mice provide an opportunity to probe mechanisms leading to motor neuron degeneration. Researchers at the University of Pennsylvania have discovered a key feature in the pathway leading to motor neuron degeneration in mice bearing a neurofilament transgene. They have found that the motor neuron degeneration is not due to the gene...

Small Molecule Inhibitor of CD4

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:16 GMT

TechID: J1574 CD4 is a transmembrane glycoprotein that functions as the major T-cell receptor for HIV-1. The CD4 receptor binds to the HIV-1 envelope protein GP120 with very high affinity. This binding is the first step in viral infection and inhibition of this step is critical for developing therapeutic methods to inhibit the disease. The three dimensional structure of the CD4 receptor was recently determined. University of Pennsylvania researchers have used computer modeling to develop constrained analogs of the CD4 receptor in order to block viral binding. These analogs can bind...

Engineering Robust Distributed Database Software

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:15 GMT

TechID: M2358 Physical data independence strives to free the query formulation process from needing to know the complex techniques that make the implementation efficient. This is a very desirable property for traditional DBMS and an essential one for information integration systems where the implementations are distributed and hidden. Researchers at the University of Pennsylvania have developed a universal query optimization technique that does not depend on physical data structure. The optimization is performed automatically through rewriting the original query using a well structured se...

Panning Phage Display Libraries Using Magnetically-Activated Cell Sorting

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:09 GMT

TechID: I1471 By panning phage display libraries using magnetically activated cell sorting, researchers at the University of Pennsylvania have developed a novel method that takes advantage of the known process of generating human antibody libraries in phages. This method, using intact red blood cells, simultaneously separates Rh(D)-positive from Rh(D)-negative red blood cells before the elution of specific Fab/phage. Use of this technique permits the identification of Fab/phage clones that are specific for most of the major Rh(D) binding epitopes. Applications &nb...

PAMM I)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:08 GMT

TechID: D622 Magnetic resonance imaging (MRI) of moving body parts, such as the beating heart, is difficult since the time constants for acquiring MRI data are long compared to the speed of motion of these body parts. University of Pennsylvania researchers have developed a technique known as Spatial Modulation of Magnetization (SPAMMÒ), which ‘marks’ heart walls so that useful MRI images can be obtained. The SPAMMÒ images are produced by first magnetically ‘marking’ the heart and then scanning the same section. By noting the displacement of the ‘marked...

Antibodies to HIV-1 & SIV proteins: AlphaHIV-1 Nef (EH1); AlphaSIVmac Nef (N27); AlphaSIVmac sp120 (DA6)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:07 GMT

TechID: P2987 Researchers at the University of Pennsylvania have developed a variety of monoclonal antibodies (all IgG1) that bind with high affinity to the HIV-1 protein, Nef. EH1 was developed in Balb/c mice inoculated with a yeast-produced Nef from HIV-1 strain, SF2. Antibodies AE6 and AG11 were similarly produced from Balb/c mice, but from bacterially produced Nef from HIV-1 strain HXBc2. All the antibodies react by western blot, immunoprecipitation, immunofluorescence assays, and are suitable for use in immunohistochemical studies of tissue sections. EH1 was s...

Novel Glucocorticoid Response Elements

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:32:06 GMT

TechID: K1672 Herpesvirus infection is characterized by an acute (productive) phase and a latent phase. Emergence from the latent phase (reactivation) can be stimulated by a number of conditions, one of which is immunosuppression that may be induced by treatment with glucocorticoids or similar steroid hormones. Researchers have defined a new type of glucocorticoid response element (GRE) in the DNA of herpesvirus family members. Binding of glucocorticoid along with its receptor to this element in herpesvirus leads to reactivation and replication of the virus. Other...

A-1-Antichymotrypsin, Analogues and Methods of Production

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:55 GMT

TechID: D601 a-1-Antichymotrypsin (A(1)AC) is a serine protease inhibitor (or serpin). Chymotrypsin-like molecules and their inhibitors (which include A(1)AC) have been identified in a wide variety of normal and abnormal biological processes including: modulation of cellular functions, DNA binding and processing of vasoconstrictor proteins and inflammation. Deficiency of serum A(1)AC has been reported to be associated with emphysema, childhood asthma, and cryptogenic cirrhosis. The role of A(1)AC in Alzheimer’s disease, cancer and wound healing is also topics of much active researc...

Molecular Candidates for a Novel Stimulatory Cardiac P2 Purinoceptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:53 GMT

TechID: K1714 The current invention comprises the identification of a novel, specific, stimulatory receptor in cardiac myocytes that mediates positive inotropic responses. The invention also embodies the use of naturally-occurring myocytes, and the development of novel recombinant myocyte cell models, for use in identifying compounds which affect the function of the receptors, and hence compounds for treatment of diseases such as congestive heart failure. The inventors also claim the use of receptor agonists in augmenting cardiac contractility in both animals and humans in a t...

Methods of Generating Human CD4+ TH2 Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:40 GMT

TechID: O2692 Graft Versus Host Disease (GVHD) is a complication that affects the immune system of patients who have received organ transplants from other individuals (allogeneic transplants). The CD4+ T lymphocyte (T cell) is the key cell mediating the human cellular immune system response against foreign transplant tissue. Purified and expanded populations of CD4+ T cells that do not recognize the foreign tissue can be used to limit or prevent the development of GVHD. Researchers at the University of Pennsylvania have created novel methods to generate and purify...

K209 Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:36 GMT

TechID: N2601 One of the most efficient and stable gene transfer vectors is recombinant adeno-associated virus (AAV). Producing AAV vectors of a high titer, quality, and potency is currently an obstacle in the development of gene therapy. Previously, all AAV vectors were derived from HeLa cell lines. These cell lines all contain genes of human papilloma viral sequences and therefore, were not suited to clinical use. K209 is a novel cell line, human derived and free of viral gene sequence, which produces high yields of stable AAV vector. As in the HeLa cell lines, t...

Inducible Mouse Models (MTB System)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:35 GMT

TechID: M2334 Mouse models of breast cancer are valuable tools for understanding the etiology of disease and for the development of novel preventive and therapeutic measures. Currently available mouse models of breast cancer express genes of interest throughout the life of the mouse. Researchers at the University of Pennsylvania have generated a novel transgenic mouse model system that permits the rapidly inducible, spatially homogeneous expression of oncogenes in the mammary epithelium of transgenic mice treated with tetracycline derivatives. This system allows regulatory molecules...

Non-Volatile Resistance-Switching Si-based Thin Film Devices

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:33 GMT

TechID: U4791 Please call licensing director for more information Applications Non-volatile memory for RAMs or data storage. - Flash memories: ~$20B - Hard disk drives: ~ $39B Advantages

Inhibitors of Cellular cdk Activities Block Replication of Herpes Simplex Virus (HSV)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:30 GMT

TechID: L1900 HSV is a common and important human pathogen, and no drugs or vaccines are currently available to prevent infection with this virus or its reactivation from latency. The inventors have established that cellular-cyclin-dependent kinases (cdks) are required for replication of HSV. Notably, no viral mutants that can replicate independently of cellular cdk activities were identified. Because cell division requires cdk activity, cdk inhibitors used as antiviral drugs in vivo would suppress not only viral replication but also cell division. The inhibition of cell divis...

Polycyclic Aromatic Compounds Having Nonlinear Optical Properties

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:26 GMT

TechID: E923 The field of photonics promises many advances in current electronics technology; by using the flow of photons instead of electrons, many functions, such as switching and computation, occur more quickly. Photonic logic may bypass some of the limitations of current electronics technology. Although photonics offers solutions to many of microelectronics’ limits, the availability of photonic materials, having desired thermal and other properties, is limited. Specifically, the technologies require materials that exhibit nonlinear optical properties in thermal operatin...

Engineering Improved DNA-Based Vaccines

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:23 GMT

TechID: I1411 In contrast to inactivated or sub-unit vaccines, which induce a humoral response, live attenuated, recombinant, and DNA vaccines all induce the cellular arm of the immune system necessary for effective treatment of pathogen infection, cancer, or autoimmune diseases. There are still limitations in vaccine technology however, with further room for improvement. It has been discovered that in vivo co-production of B7 proteins in cells expressing targ...

Substituted Porphyrins, Porphyrin-Containing Polymers, & Synthetic Methods Therefor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:23 GMT

TechID: E960 Researchers at the University of Pennsylvania have built a comprehensive portfolio of patents and patent applications for creating nanoscale structures called emissive polymersomes. Emissive polymersomes are innovative nanoscale agents that enable a wide range of unique therapeutic, diagnostic, vaccine delivery, and imaging applications. Relative to other nanoscale structures that possess in vitro utility, emissive polymersomes are exceptional in that they enable a facile transition from in vitro diagnostic applications to in vivo targeting, imaging, and treatmen...

Nanotubes and Nanofilaments Dispersion in Thermoplastics and Thermosets by In-Situ Polymerization Methods

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:20 GMT

TechID: O2802 There have been numerous and varied attempts to disperse carbon nanotubes in thermoplastic polymers. Although solvent-based and mixing-based approaches have shown some success, results show these methods are not ideal means of obtaining versatile polymers with a uniform dispersion of nanotubes. Accomplishing this would open up the market to these potentially very valuable composite materials. Researchers at the University of Pennsylvania have established a novel method for manufacture of dispersed, aligned polymer-nanotube composite materials. The r...

Gated Programmable Ventilation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:10 GMT

TechID: D829-X A recurrent problem in the medical and surgical intensive care field is the patient with coexistent respiratory and cardiac failure. Since cardiac and ventilator devices are not synchronized to work with each other, augmenting one system may actually diminish the effectiveness of the other system. To prevent this problem, University of Pennsylvania researchers have developed a non-invasive device for improved treatment of patients on ventilators. This device, called the gated programmable ventilator, simultaneously monitors the patient’s electrocard...

Adenovirus Packaging Cell Lines

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:08 GMT

TechID: H1341 Researchers at the University of Pennsylvania have developed a novel packaging cell line which expresses adenovirus genes E1a, E1b and E4, or functional fragments. In one embodiment, the E4 gene fragment is open reading frame (ORF) 6 under the control of an inducible promoter. This cell line has provided recombinant adenovirus with functional deletions of the E1 and E4 gene regions; a suitable gene operatively linked to regulatory sequences directing its expression, and an adenovirus capsid which allows the recombinant virus to infect mammalian cells and e...

Magnetic Resonance - Electrical Impedance Tomography

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:06 GMT

TechID: K1687 Measurements of the electrical properties of human tissues can provide anatomical images of the body and information about the pathological state of tissue. Electrical impedance tomography (EIT) and magnetic resonance current density imaging (MRCDI) are two techniques currently used to measure the electrical properties of human tissue. EIT produces images of the spatial distribution of impedance of a body by applying current to one set of electrodes and then measuring the voltage differences between adjacent sets of electrodes. However, this techniqu...

Therapeutic Application of Incretin Antagonists in Subjects withi Hypoglycemic Disorders

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:05 GMT

TechID: T4394 Glucagon-like peptide-1 (GLP-1) is a gastrointestinal hormone that stimulates insulin secretion, inhibits glucagon secretion, and slows diabetes progression. Exendin [9-39] amide is a potent peptide antagonist of the Glucagon-like peptide-1 receptor, and is useful for reversing the effects of GLP-1 agonists. Ongoing Clinical Study: An Open Label Pilot Study of the Effects of the Glucagon-Like Peptide-1 Receptor Antagonist, Exendin-(9-39) on Glycemic Control in Subjects With Congenital Hyperinsulinism. Detailed Description: This is an open-label, pilot study , to dete...

Identification of Murine and Human OCL-5G10

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:04 GMT

TechID: O2711 Osteoporosis, which affects 10 million people in the United States alone, is a disease in which the density and quality of bone are reduced, leading to weakness of the skeleton and increased risk of fracture, primarily in hip, spine and wrist. Bone is continuously remodeled through new bone formation by osteoblasts and the resorption of old bone by osteoclasts. Osteoporosis develops when bone resorption occurs too quickly or if replacement occurs too slowly, therefore therapies that disrupt the activity of osteoclasts can be useful in slowing the progressi...

Anti-OSCAR Monoclonal Ab

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:31:00 GMT

TechID: P2931 Bone is continuously remodeled through new bone formation by osteoblasts and the resorption of old bone by osteoclasts (OCs). OCs are derived from bone marrow precursor cells of monocyte-macrophage lineage, and osteoclastogenesis requires M-CSF and TRANCE, two essential factors produced by osteoblasts. University of Pennsylvania researchers have identified OC-associated receptor (OSCAR), a novel member of the leukocyte receptor complex (LRC)-encoded family expressed specifically in OCs. There is evidence indicating that OSCAR may be an important bone-specific regulato...

Cellulose-based hyrdogels for soft tissue regeneration

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:58 GMT

TechID: U4821 Articular cartilage is a hydrated, soft connective tissue that functions to redistribute applied loads and to provide a low friction-bearing surface in moveable joints. Annually, over one million procedures involving cartilage replacement are performed in the United States as a result of debilitating ailments affecting articular cartilage. The most common of these pathological conditions is osteoarthritis (OA), a degenerative joint disease that afflicts between 32-38 million Americans. OA is characterized by a progressive loss of cartilage tissu...

Human Hematopoietic Stem Cell Receptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:58 GMT

TechID: G1097 Lymphohematopoietic stem cells (LHSC) are the progenitors for virtually all blood cells, yet make up only ~0.01% of human and murine marrow cells (Till et al., Biochem. Et Biophys. 1980. 605: 431-459). The ability to isolate and expand this population would facilitate the study of early hematopoietic development with clinical implications such as bone marrow transplantation for cancer and genetic disease. CD34+ cells contain nearly all of the progenitor activity of marrow and include (LHSC). To d...

Alignment of Single-wall Carbon Nanotubes: Nematic Nanotube Gels (Combined into P2952)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:55 GMT

TechID: P2984 Single-Wall Carbon Nanotubes (SWNT) possess anisotropic electrical conductance and extraordinary high thermal conductance, which makes them by far the best heat conducting matter known to date. Composite materials containing SWNT may display some of these anisotropic properties if the nanotubes are properly aligned within the composite. However, alignment of nanotubes in composites in a controlled manner presents a significant technical challenge. Researchers at the University of Pennsylvania have developed three new techniques for aligning SWNT composite materials based o...

A Motor Neuron Cell Line with Reduced SMN: A Model System for Spinal Muscular Atrophy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:54 GMT

TechID: L1906 Spinal Muscular Atrophy (SMA) is a degenerative disease of motor neurons, resulting in progressive weakness and muscular atrophy. It is the most common genetic cause of infant mortality. Mutations in or deletions of the Survival Motor Neurons (SMN) gene are responsible for SMA, and the level of SMN function in a cell is closely correlated with the severity of the disease. Although expressed in all cells, levels of SMN in the spinal motor neurons of unaffected individuals are much higher than in the res...

Factor VIII Gene Therapy Targeted to Epidermis of the Skin

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:54 GMT

TechID: K1817 Researchers at the University of Pennsylvania have established proof of principle that the epidermis of the skin can serve as a target tissue for gene therapy for hemophilia A. These investigators have generated transgenic mice that direct expression of human factor VIII to the epidermis. The factor VIII transgene expression is restricted to the target tissue (skin), although some ectopic expression in other stratified squamous epithelia is also observed. The proof of therapeutic application of this approach of gene therapy is established in experimental mo...

In Vitro Derivation of Germ Cells from Mouse Embryonic Stem Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:52 GMT

TechID: N2577 In vitro techniques to derive germ cells have been used to provide a potential source of donor cells for fertility applications. Previous efforts to generate germ cells from embryonic stem cells in culture have failed. Recently, researchers at the University of Pennsylvania were able to derive germ cells from mouse embryonic cells using cell culture and selection techniques. In one technique germ cells were derived from embryonic stem cells (ES) and in the other the cells were derived using embryoid bodies. Gene transformation techniques were u...

Cardiac Output from Single Breath CO2 Lung Washout

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:51 GMT

TechID: J1481 Cardiac output, the heart's blood flow rate, is integral to assessing cardiac performance. This measurement determines the extent of cardiac and pulmonary disease and monitors the effectiveness of treatment protocols. Unfortunately, present methods for determining cardiac output are invasive. Some techniques require venous catheterization, injection proximate to right atrium, and arterial catheterization. Due to the invasive nature of these procedures, adverse medical consequences often result. Additionally, these techniques are limited by their complex procedures re...

Function and Regulation of Angiopoietin 3

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:49 GMT

TechID: P3172 Angiopoietins have a crucial regulatory role in angiogenesis during both normal development and tumor growth. Four angiopoietins (Ang) have been identified to date. The role of Ang-1 and Ang-2 as regulators of angiogenesis during both vascular maintenance/development and pathologic states such as cancer has been documented. However, very little is known about Ang-3 and its human ortholog, Ang-4. Researchers at the University of Pennsylvania have discovered several novel functions and regulations of Ang-3 which define its role as a potential marke...

HSV-1 Glycoprotein C (gC) - Monoclonal Antibodies and Expression System

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:47 GMT

TechID: O2888 The envelop of herpes simplex virus (HSV) contains at least 11 virus-encoded glycoproteins. Glycoproteins C (gC) is involved in virus infection of host cells. In addition, gC functions to downregulate the host antiviral immune response by binding and sequestering complement component C3b. The gC protein is associated with the viral envelop via a transmembrane domain and the vast majority of gC is exposed on the outside of the virion. University of Pennsylvania researchers have developed monoclonal antibodies against HSV-1 glycoprotein C and an expression system...

Enantioselective Indole Claisin Rearrangement

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:46 GMT

TechID: U4864 Researchers at the University of Pennsylvania have discovered a novel and efficient method to synthesize enantiopure oxindoles containing quaternary centers. These compounds incorporate a chiral oxindole moiety which is a known building block for several pharmaceutical drugs presently on the market. Furthermore, these oxindoles backbones have also been found in natural products with diverse biological activities that such as physostigmine, flustramine B, mollenine A etc. Previously known methods to generate oxindoles with quaternary stereo centers have limitations ...

Peptide-Directed Degradation of Urokinase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:44 GMT

TechID: K1668 The urokinase plasminogen activator (uPA) is required for the generation of plasmin, which plays a critical role in hemostasis, inflammation, atherosclerosis and tumor development. The uPA protein also has a role in cell migration via its binding with the adhesive protein vitronectin. In vivo, uPA exists in several molecular forms, one of which is the single chain protein (scuPA). The scuPA form undergoes a reversible conformational change when it binds to its receptor that endows it with enzymatic activity, protects it from inactivation by plasminog...

Chimeric Envelope Proteins for Viral Targeting

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:38 GMT

TechID: E851 Viral particles, and in particular retroviruses, continue to be a major focus for use as delivery vehicles for gene therapy or other biological mediators. If the cellular host range can be tailored to a cellular subset of one's choosing, then the delivery of biological mediators can be expanded or restricted to those cells which would benefit. In order to demonstrate this process, Dr. David Weiner and his research group at the University of Pennsylvania have developed a model to target Granulocyte -Macrophage Colony Stimulating Factor (GM-CSF) to its recept...

129Xe MRI Contrast Agents for Molecular Imaging and Early Detection of Breast Cancer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:24 GMT

TechID: R3978 This body of work describes an enzyme-responsive 129Xe biosensor in which a matrix metalloprotease (MMP-7) specific peptide substrate is attached to cryptophane-A. Nuclear magnetic resonance (NMR) spectroscopy offers tremendous opportunities for high-resolution, minimally-invasive, molecular imaging of buried tissue for the early diagnosis and treatment of cancer. However, low sensitivity and complex background signals compromise biomarker detection. Recently, laser-polarized 129Xe has gained attention as an MR probe, due to its large signal (10-70% alignment of Xe nuclear ...

Fabrication of Nanotube-Epoxy Composites with Enhanced Thermal Conductivity

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:23 GMT

TechID: O2686 Researchers at the University of Pennsylvania (Penn) have developed a novel method to manufacture polymer composites with a family of nanomaterials, which are applicable to thermal management. Penn is seeking a partner for the commercialization of this technology and who will sponsor short-term research leading to broader composition of matter. Penn's composites are the best artificial thermal conductors prepared to date. They are processable in accordance with the properties of the parent polymer matrix (thermosetting or thermoplastic). Depending on the...

Antibodies to the Fragile X Protein

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:22 GMT

TechID: G1135 The gene directly responsible for Fragile X Syndrome, FMR1, is located on the X chromosome and is likely an RNA-binding protein. Two novel human genes are highly homologous to FMR1, encode for RNA-binding proteins, but are not located on the X chromosome. Currently employed nucleotide probes or antibodies to detect the FMR1 gene or its product may cross-react with these two related genes and proteins, leading to a false positive diagnosis. University of Pennsylvania researchers have develop...

Polarization - Difference Vision

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:20 GMT

TechID: E865 Suspended particles (i.e. fog, rain, plankton) make visibility and visual contrast extremely limited in scattering media. Two University of Pennsylvania researchers have developed an effective solution: polarization difference imaging (PDI) system. Based on polarization differentials, the PDI system improves the detectability of objects by enhancing image contrast via a reduction of background light. The fundamental basis of this imaging technology is the difference in the degree of linear polarization of background light in comparison to that of the target object. T...

Mouse Model of Multiple System Atrophy a-Synuclein Expression in Oligodendrocytes Causes Glial and Neuronal Degeneration

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:18 GMT

TechID: R3934 Nature Applications Advantages

Brain Activity During Deception

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:17 GMT

TechID: N2598 Deception is a common cognitive phenomenon with important practical implications. The known correlation between the P-300 component of evoked brain potentials and simulated deception suggests that cognitive distinction between truth and lie could be associated with detectable differences in local brain activity. The guilty knowledge task (GKT) has been used extensively to simulate deception under laboratory and field conditions. An association between the evoked brain potentials and lying during ...

Synthesis and Biological Activity of the Triene Side-Chain Models of Spongistatins

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:17 GMT

TechID: K1740 The current invention describes the synthesis and biological activity of side-chain models of the spongipyrans. These spongipyrans appear to be the most potent inhibitors of cancer cell growth discovered to date. The extreme scarcity from natural sources, unprecedented cytotoxicity, and structural complexity of these metabolites provide strong impetus for improved synthetic methods and/or less complex compounds having similar levels of cytotoxicity. The present invention provides compounds which mimic the chemical and/or biological activity of the spongis...

Novel Porphyrine and Porphyrin Synthesis Techniques

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:16 GMT

TechID: G1194 Current synthetic methods for producing porphyrins produce low yields and do not exist for many types of porphyrins. University of Pennsylvania researchers have developed an improved method for synthesizing a wide variety of electron deficient and strapped porphyrins. Applications Advantages

Support for High Performance Liquid Chromatography In A Magneticaly Stabilized Fluidized Bed

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:14 GMT

TechID: D630 Obtaining a purified product from a batch chromatographic separation demands that the feed be injected into the system for only a fraction of the total running time. This discontinuous introduction of feed leads to inefficient and labor intensive systems. Continuous sample injection and product withdrawal addresses the efficiency problem of batch operations but raises issued of expensive moving seals and the risk of contamination is high when the feed stream contains biological materials. A magnetically stabilized fluidized bed (MSFB) has many unique proper...

Novel Predictors of Cardiac Allograft Rejection Determined by Peripheral Blood Gene Expression Profiling

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:13 GMT

TechID: Q3426 Endomyocardial biopsy is the standard for detecting cardiac allograft rejection, but it is limited by invasiveness and cost. Researchers at the University of Pennsylvania have discovered a novel process that may well predict cardiac allograft rejection using only peripheral blood samples from patients. Utilizing oligonucleotide microarrays to detect gene expression profiles in peripheral blood samples, they can predict cardiac graft rejection. Presently, they have discovered a panel of novel candidate predictors associated with the presence of car...

Fabrication of Nanowires and Nanogaps for Molecular Electronic Circuitry

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:08 GMT

TechID: M2171 Researchers at the University of Pennsylvania have developed a novel method of fabricating nanogap metallic wires and wires with nanometer size breaks. This design enables molecules to attach across the nanogaps resulting in a complete circuit. Integral to the fabrication process is carbon nanotubes (NTs). These nanotubes are used as lithographic masks to create regulated gaps ranging in size of 1nm [diameter of C single-walled nanotube (SWNT)] to 50nm [diameter of C multi-walled nanotube (MWNT)] in the metal wire. SWNT result in narrow nanogaps and MWNT are used to ...

Novel Antibodies to the Human Thrombin Receptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:30:06 GMT

TechID: F1089 Thrombin is able to evoke biological responses from a variety of cells that are normally located in or around the vascular space, including platelets, monocytes, endothelial cells, fibroblasts, and vascular smooth muscle. Recent evidence suggests that most, if not all, of these responses are mediated by cell-surface receptors belonging to the family of G protein-coupled receptors. According to current models, human thrombin receptors are activated when thrombin cleaves the receptor's N terminus, exposing the tethered ligand domain, SFLLRN. The first and most wide...

Therapeutic and Diagnostic Applications of Mouse Ascites Golgi (MAG) Manipulation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:57 GMT

TechID: D832 Certain glycoproteins produced by epithelial cells are synthesized and secreted in a menstrual cycle dependent fashion. University of Pennsylvania researchers have developed therapeutic and diagnostic applications for one such mucin glycoprotein produced by human endometrial glands. The researchers have produced antibodies that are reactive with this glycoprotein and can be used to detect it in biological samples. Endometrial or reproductive fluid samples can be assessed for relative concentrations of this glycoprotein. Greater concentrations of...

Mice with Inducible Loss of Pancreatic Beta-cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:51 GMT

TechID: N2524 The current standard diabetic model is the streptozocin (STZ)-induced diabetic mouse where high dose STZ induces pancreatic B cell necrosis. This is not an easily controlled procedure and can result in animal death or only partial loss of beta cells in cases of inadequate administration. There remains the preference for an improved diabetic animal model. Researchers at University of Pennsylvania are developing two transgenic (genetically altered strains) ...

A Method to Inhibit HSV Infection by a Novel Peptide Against a Functional Domain of HVEM Receptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:50 GMT

TechID: K1729 Statistically, one out of three Americans carry the HSV-1 or HSV-2 in the dormant state, ready to be activated when the host is stressed or immunocompromised. Currently there are no fully effective treatments for a herpesvirus infection in humans. The identification of compounds that inhibit entry of the herpesvirus into cells would greatly facilitate treatment of herpesvirus infection in humans. The present invention is based upon the discovery of two cellular proteins which mediate entry of HSV-1 and/or HSV-2 and other herpesviruses into cells. The...

Small Pox Inhibitor of Complement Enzymes (Spice) Protein and Methods of inhibiting Complement Activation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:46 GMT

TechID: L2028 The complement system plays a fundamental role in both the innate and acquired immune responses. It is involved in the majority of diseases characterized by acute and/or chronic inflammation, as well as hyperacute rejection of xenografts. The human body has a family of regulatory molecules which bind and inhibit complement components and thereby protect cells and tissues from inadvertent complement-mediated injury. Researchers at the University of Pennsylvania have created a protein that has a greater complement-inhibiting activity than VCP (Vaccinia...

DR5 Knockout Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:44 GMT

TechID: Q3589 DR5 (also called TRAIL receptor 2 and KILLER) is an apoptosis-inducing membrane receptor for tumor necrosis factor-related apoptosis-inducing ligand (also called TRAIL and Apo2 ligand). DR5 is a transcriptional target of p53, and its overexpression induces cell death in vitro. However, the in vivo biology of DR5 has remained largely unexplored. To better understand the role of DR5 in development and in adult tissues, we have created a knockout mouse lacking DR5. This mouse is viable and develops normally with the exception of having an enlarged thymus. We show that DR5 is no...

Shared Specificity Monoclonal Antibodies Against Both p185 her2/neu and EGFr

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:42 GMT

TechID: N2457 Significant time and money have been spent searching for mammalian cancer tumor treatments. Current tumor treatments rely on the cytotoxic effects of drugs and radiological therapy. Although these treatments bring remission and cure to some patients, they unfortunately have serious side effects because they kill not only tumor cells but also normal non-tumorous cells. Thus treatments targetting only tumor cells are most desirable for the best results. Recently University of Pennsylvania researchers have developed methods of treating and diagnosing mammalian cancer tum...

Novel Design for Temporary Pacing Wires for Cardiac Surgery

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:39 GMT

TechID: J1582 The removal of temporary pacing wires after open heart surgery has been known to cause cardiac tamponade in approximately 1/2% of all cases as well as other serious complications. During open heart surgery, one or two pairs of temporary pacing wires are placed on the patient's myocardium in order to prevent transient heart block. These wires are then removed (pulled out through the heart muscle) on the third or fourth postoperative day. Any resulting tear to the heart muscle can lead to serious complications such as cardiac tamponade and even death. A University of Pennsyl...

Oligomeric Anilines and Their Synthesis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:36 GMT

TechID: J1631 Polyanilines are well known conductive polymers. While the synthesis of several smaller tetrameric oligomers has been reported, the preparation of larger oligomers has proven elusive. The present invention affords a method to prepare higher oligomers of aniline in several distinct oxidation states. Applications Advantages

Method of Delivery of Genes to Muscle

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:35 GMT

TechID: J1624 Although a number of gene therapy systems have been developed utilizing viral vectors, the issues of delivery and uptake by specific tissues have been problematic. This is particularly true with respect to gene therapy targeting diseases affecting muscle tissue, such as muscular dystrophy. This is because different sections of skeletal muscle are not uniformly perfused. Researchers at the University of Pennsylvania have developed a method of increasing endothelial permeability for gene transfer procedures. This technology overcomes the potentially dangerous...

A Method for Multi-slice Perfusion Imaging with MRI

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:33 GMT

TechID: J1546 Perfusion imaging maps blood flow rates in tissue and is employed to diagnose and evaluate strokes, pulmonary embolism, cancer, and dementing diseases. University of Pennsylvania researchers have developed a multi-slice magnetic resonance (MR) imaging technique to produce perfusion images that are insensitive to blood-arterial transit times. Previous MR perfusion imaging techniques isolated the clinically significant blood flow rate in tissue from arterial flow rates with prohibitively slow single slice imaging. Multi-slice perfusion imaging dramatic...

A Method for Encapsulating Biological Molecules and Materials in a Porous Inorganic Matrix

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:30 GMT

TechID: M2184 Encapsulation of biologically active molecules has generated considerable attention particularly in the fields of medicine and biotechnology. Encapsulation of biologically active materials using inorganic matrices is particularly attractive because it combines the physical and chemical stability of the matrix with the high activity and specificity of biomolecules. However, the present methods for entrapment are inadequate. Thus, University of Pennsylvania researchers have successfully created an aqueous colloidal sol-gel method to encapsulate biologically active ...

NS-123 and NS-160, Two Novel Radiation Sensitizers for Malignant Tumor Cells Identified through an Unbiased Screening of a Commercially Available Chemical Library

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:29 GMT

TechID: U4596 For patients with solid tumors, the tolerance of surrounding tissues often limits the dose of radiation that can be delivered. Thus, agents that preferentially increase the cytotoxic effects of radiation toward tumor cells would significantly alter the therapeutic ratio and improve patient survival. Using a high throughput, unbiased screening approach, we have identified 4-bromo-3-nitropropiophenone (NS-123) as a radiosensitizer of human glioma cells in vitro and in vivo. NS-123 radiosensitized U251 glioma cells in a dose-dependent and time dependent mann...

Apparatus to Measure Blood Flow Using Ultrasound-Contrast-Enhanced Gray-Scale and Doppler Images

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:23 GMT

TechID: M2174 Angiogenesis, or new vessel formation, occurs through tightly regulated processes under normal physiological situations. Under pathological conditions, such as inflammation and neoplastic formations, these tight controls are breached with ensuing uncontrolled angiogenesis from existing vasculature. With such conditions there is a need to measure blood flow through new and existing blood vessels. Although current methods of Doppler ultrasound measure blood flow through relatively larger vessels, t...

3-Dimensional Localized Proton NMR Spectroscopy Using a Combination of Hadamard and Chemical Shift Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:21 GMT

TechID: J1590 Proton magnetic resonance spectroscopy (MRS) studies of organs such as the brain require suppression of subcutaneous lipids and bone marrow signals to prevent contamination of the much smaller brain metabolite signals. This is achieved by a combination of outer volume suppression, which localizes a plane in the brain, and 2-D chemical shift imaging within this plane. Interleaving successive slices generates a complete image of the brain. Because the transverse relaxation times, T2, of brain metabolites are long, this technique suffers from long acquisition times...

Compstatin Analogs with Improved Activity#2

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:16 GMT

TechID: P2942 Please call licensing director for more information Applications Prevents an autoimmune response induced by the complement pathway Advantages Prevents activation of both the classical and alternative complement pathways Targets an extracellular and early step during the activation of complement Compound is a small 13 residue peptide

Method for Generating Optimal Pulses for Magnetic Resonance Imaging Using Spatial Modulation Magnetization

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:14 GMT

TechID: D812 Magnetic resonance imaging (MRI) of moving body parts, such as the beating heart, is difficult since the time constants for acquiring MRI data are long compared to the speed of motion of these body parts. University of Pennsylvania researchers have developed a technique known as Spatial Modulation of Magnetization (SPAMMÒ), which ‘marks’ heart walls so that useful MRI images can be obtained. The SPAMMÒ images are produced by first magnetically ‘marking’ the heart and then scanning the same section. By noting the displacement of the ‘marked’ areas...

Transgenic Mice Containing the bcl-2 Oncogene

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:09 GMT

TechID: F1073 Bcl-2 protooncogene is normally expressed in both mature B and T cells. However, this gene is over expressed in human follicular lymphomas and has been found to extend the cellular lifespan of these cancerous cells. Bcl-2 is unique among oncogene products in that it appears to enhance lymphoid cell survival by interfering with programmed cell death (also known as apoptosis) rather than cell proliferation as seen in many other oncogenes. However, the physiological role of the Bcl-2 protein in lymphocyte development is unclear. Thus, more tools are needed to better under...

Methods and Compositions to Protect Heart from Ischemia by Administering Adenosine A1/A3 Receptor Agonists

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:04 GMT

TechID: J1602 Adenosine is known to decrease the size of myocardial infarctions caused by ischemia. How and when this cardioprotective effect occurs is beginning to be elucidated. Researchers at the University of Pennsylvania have shown that A1 and A3 adenosine receptors specifically mediate a cardioprotective effect on myocytes. They have now discovered that selective adenosine A2a receptor antagonists can also achieve significant myoprotective results as well. Using an in vitro model of simulated ischemia, cell death was...

A Mouse Model of Hemophilia A

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:04 GMT

TechID: H1264 Hemophilia A is an X chromosome-linked bleeding disorder that affects 1 to 2 individuals in 10,000 male births and is caused by defects in the coagulation factor VIII gene. Roughly 60% of patients have severe disease with factor VIII activity less than 1% of normal level. These patients have frequent spontaneous bleeding into joints, soft tissues, muscles and internal organs, and require regular intravenous injections of plasma-derived or recombinant human factor VIII. However, this treatment is expensive and could lead to life-threatening complicati...

Dehydrotamandarin A

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:02 GMT

TechID: M2162 Plants and marine organisms have become excellent sources of novel compounds that can be used therapeutically in humans. Tamandarin A is related to a class of molecules known as didemnins, molecules that are harvested from marine organisms and have been found to be potent immunosuppressants and inhibitors of tumor cell growth. Their unwanted side effects, however, have limited their utility as therapeutics. Importantly, it has been found that side chain modification of the didemnins can improve their efficacy at killing tumor cells and as immunosuppressants, and...

A Mechanism to Modulate Circadian Rhythms

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:29:01 GMT

TechID: N2551 The master circadian clock in mammals is located in the suprachiasmatic nuclei (SCN) and drives daily variations in many physiological and behavioral processes. Recently, molecular clocks similar to those operating in the SCN neurons have been uncovered in peripheral tissues such as the Liver and Kidney. Researchers at the University of Pennsylvania are the first group to demonstrate the presence of a molecular clock in the vasculature. They also demonstrated that the nuclear receptors, RARalpha and RXRalpha, interact with the positive circadian regulators...

Novel Monoclonal Antibodies Demonstrate Biochemical Variation of Brain Parkin with Age

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:57 GMT

TechID: Q3469 Autosomal recessive juvenile parkinsonism is a movement disorder associated with the degeneration of neurons. The loss of functional parkin caused by parkin gene mutations is the most common cause of juvenile parkinsonism. The studies on parkin have been impeded because of limited reagents specific for this protein. Researchers at Penn have developed a panel of parkin-specific monoclonal antibodies that recognize different domains of parkin and their specificity in murine and human brains was demonstrated (Journal of Biological Chemistry, 278, 48120-48128, 2003). Applic...

Mouse PancChip

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:56 GMT

TechID: Q3255 Despite continuous efforts in diabetes research, tools for the treatment of diabetes have not changed significantly. The identification of endocrine precursor cells and factors that regulate the development and differentiation of beta-cells is still a major hurdle. Researchers at the University of Pennsylvania have assembled a non-redundant pancreatic mouse cDNA clone set which represents the vast proportion of the pancreatic and islet gene expression profile. This clone set represents 13,000 non-redundant clones which were thoroughly characterized by s...

Simplified Use of '5-Prime Ends of RNAs (SUPER) for Cloning

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:55 GMT

TechID: I1404 The technology enables the user to construct cDNA libraries from RNA, employing a 5'-end replication technique. The method is more simple and prolific than currently practiced RNA replication technologies. The method may be streamlined into a kit with instructions, for use in high throughput lab research or diagnostics. Applications replication technology for cloning post-processing mRNA sequences complements proteomics research Advantages rapid cDNA library management full platform for development into a kit

Mutant Tau Transfectants Develop Tau Aggregates

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:54 GMT

TechID: M2267 Tau is a common microtubule associated protein in the central nervous system that is primarily expressed in neurons and implicated in the pathogenesis of Alzheimer’s disease, parkinsonism, and related neurodegenerative diseases known as tauopathies. It is believed that multiple tau gene mutations impair the ability of their tau products to bind or promote the assembly of microtubules and result in filamentous aggregates instead. Until recently it had not been possible to produce tau aggregates in intact cells...

Methods for Protecting Organs from Ischemia by Simulating Preconditioning and by Administration During Ischemia

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:47 GMT

TechID: J1517 Researchers at the University of Pennsylvania have determined that cardiac myocyte death from myocardial infarction can be decreased by ischemic preconditioning with certain biologically active molecules. This invention describes novel methods and compositions that allow the: 1) preconditioning of heart tissues against ischemia; 2) either adjunct therapy with thrombolysis or acute administration during myocardial infarction to reduce infarct size and salvage potentially viable myocardium. Applications Advantages

Corticotropin Release Inhibitor Factor (CRIF)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:47 GMT

TechID: G1191 University of Pennsylvania researchers have discovered a hormone, corticotrophin release inhibiting factor (CRIF), that regulates the release of adrenocorticotropic hormone (ACTH), the sole regulator of glucocorticoid secretion in response to stress. While other research in this area is focused on candidates that each inhibit only one of several known stimulators of ACTH, CRIF directly inhibits synthesis and secretion of ACTH itself. Regulation of CRIF may lead to new strategies for treatment of a wide variety of conditions such as mood disorders (includin...

Methodological, Diagnostic and Therapeutic Applications of Organotypic (3D) Culture

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:40 GMT

TechID: U4594 Technology Summary: Introducing a highly innovative, in vivo-like, three-dimensional culture system useful for screening anti-cancer compounds, metastasis suppressors, determining pharmacological profiles of given compounds, and for identifying novel biomarkers. Intellectual Property: 60/984,190 filed October 31, 2008, titled "Culture based screening assay and methods of use thereof to identify agents which modulate tumor development, invasion, and differentiation", by Anil Rustgi. Publications: Okawa T et al. (2007). Genes & Development; 21:2788-2803. This t...

Post Transcriptional Gene Silencing (PTGS)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:39 GMT

TechID: N2427 RNA interference (RNAi) is an evolutionarily conserved post-transcriptional gene silencing mechanism involved in defense against viruses. It is triggered by double stranded RNA (dsRNA), which after conversion into short RNAs, directs ribonucleases to degrade the corresponding homologous mRNA targets. RNAi has become an important tool for the analysis of gene functions in invertebrate organisms. However, when applied in vertebrate species, RNAi was found to be less predictable and inefficient. University of Pennsylvania researchers have developed a ...

Non-Peptide Peptidomimetics

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:36 GMT

TechID: D823 Peptides are important in many biochemical processes in humans. Two classes of peptides, hormones and neurotransmitters, are controlled by receptor-mediated stimulation of regulatory proteins called G proteins. Examples of G protein-linked receptors include receptors for somatostatin, catecholamines and substance P. Synthetic peptides could mimic or inhibit biological and/or chemical activity of the peptides that bind G protein-linked receptors. However, limited therapeutic applications exist involving peptides due to poor oral bioavailability a...

Prevention of Tumors with Monoclonal Antibodies Against Neu

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:32 GMT

TechID: F1062 University of Pennsylvania researchers have developed a method to prevent mammalian tumors which contain cells expressing the neu gene product, p185. p185 is a transmembrane glycoprotein with tyrosine kinase activity. p185 has been found active in human adenocarcinomas including breast, lung, salivary gland, and kidney adenocarcinomas, as well as prostate neuroblastoma. In addition, p185 was found in 30% of all primary breast cancers examined. Increased stage of malignancy and reduced survival time were both directly correlated with an increased leve...

Heat shock proteins HSP70 and HSP27 in the cerebral spinal fluid of patients undergoing thoracic aneurysm repair correlate with the probability of postoperative paralysis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:24 GMT

TechID: U4811 Researchers recently completed a study indicating that Heat Shock Proteins HSP70 and HSP27 are biomarkers that can detect acute spinal cord ischemia during thoraco-abdominal aortic aneurysm surgery. Study results also indicate that pre-operative measurement of these same biomarkers may predict an elevated risk of paralysis in patients being considered for aneurysm repair and cardiopulmonary bypass surgery. Despite clinical efforts to prevent spinal cord ischem...

Drosophila System for Screening for Genes and Agents that Affect Triplet Repeat Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:22 GMT

TechID: K1810 Certain human neurodegenerative diseases, such as Huntington’s chorea, are characterized by excessive CAG triplet repeats within the DNA of afflicted individuals. The normal form of the protein contains around 20-30 glutamine repeats (coded for by the CAG triplet), whereas diseased protein may contain 70 or more glutamines. University of Pennsylvania scientists have genetically-engineered a mutant form of the Drosophila melanogaster (fruit fly) which exhibits traits of an individual with the CAG triplet repeat disorder. This particular disease model, developed using a robus...

Animal Model for Screening Anti-Diabetic Drugs

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:22 GMT

TechID: J1588 Non-insulin-dependent diabetes mellitus (NIDDM) is characterized by peripheral insulin resistance, increased hepatic glucose production, and defects in insulin secretion from pancreatic ß cells. Although the underlying cause of these defects in the majority of patients with NIDDM is unknown, there is a strong correlation with obesity. In previous experiments conducted by researchers at the University of Pennsylvania, transgenic animals were created which were characteristically resistant to obesity. These mice were completely resistant to chemically-induced...

Microstructure and Behavior of Fullerenes Contained Within Single Wall Carbon Nanotubes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:18 GMT

TechID: L2033 University of Pennsylvania researchers have developed a novel method to functionalize nanotubes via filling: an operation that inserts appropriately sized gas phase molecules into the nanotube matrix. Utilizing this method, researchers were able to produce a new class of nanotube-fullerene hybrids. The nanotube-fullerene hybrids are composed of C60 molecular chains inside single-wall carbon nanotubes (SWNTs). These hybrids can be synthesized using SWNTs that were created using any method including pulsed laser vaporization or carbon arc techniques. After...

Diffusion Imaging with a Modified CPMG Sequence

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:13 GMT

TechID: J1545 University of Pennsylvania researchers have developed an MRI technique which generates high resolution, artifact-free diffusion images. Until recently, diffusion imaging has been of limited use as a diagnostic technique because it generated images which were prone to motion artifacts. The present invention removes these artifacts by applying a modified Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with judiciously chosen gradients and radiofrequency (RF) pulses used to eliminate phase errors. Applications Advantages

Molecular Correlates of Schizophrenia

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:08 GMT

TechID: K1854 Schizophrenia is a chronic debilitating psychiatric disorder that affects approximately 1% of the general population. Evidence suggests that this disorder is caused by alterations in the expression of genes in discrete regions of the brain. However, distinct neurobiological markers that are specific for schizophrenia have remained elusive. Researchers at the University of Pennsylvania have recently identified several genes and expressed sequence tags (ESTs) that are differentially regulated in the entorhinal cortex (EC) of individuals diagnosed with schizophrenia...

Recombinant Murine Coronavirus Expressing the Enhanced Green Fluorescent Protein

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:04 GMT

TechID: P3194 Robust assays are needed to screen for inhibitors against coronaviruses, such as the virus responsible for Severe Acute Respiratory Syndrome (SARS). Current assays for murine coronavirus infection are cumbersome, laborious, and not amenable to high throughput screening. Researchers at the University of Pennsylvania have developed a fluorescence-based assay for coronavirus infection using viruses that stably express enhanced green fluorescent protein (EGFP). As a result, these viruses can be monitored in living cells in real time, an approach that is amenable to h...

CX2.1 (AlphaHLA-Class II)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:28:00 GMT

TechID: P2986 Researchers at the University of Pennsylvania have a mouse monoclonal antibody (IgG2a) which recognizes all Class II HLA surface antigens. The antibody is specific for the conserved framework of Class II HLA and therefore binds with high affinity to HLA-DR, DP, and DQ subtypes. The antibody has been effective in cell sorting assays using FACS and can elicit complement-mediated killing of Class II HLA positive cells. The antibody was produced from SJL mice inoculated with human acute myelogenous leukemia blasts. This novel antibody may be useful as a researc...

Enhancement of AAV Expression Gene Therapy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:59 GMT

TechID: H1337 A method for enhancing the efficiency of transduction of a recombinant AAV into a target cell has been developed by researchers at the University of Pennsylvania. The target cell is infected with a recombinant adeno-associated virus comprising a selected transgene under the control of regulatory sequences. The infected cell is contacted with an agent which facilitates the conversion of single stranded recombinant virus to its double stranded form. When this conversion occurs in the target cell, enhanced transduction of the recombinant virus into said target cell resul...

Efficient Nuclear Delivery of Antisense Oligonucleotides in vitro and in vivo by Neutral, Nano-Transforming Polymersomes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:45 GMT

TechID: T4373 For gene therapy, cancer treatment and other therapeutic applications, there is a need for drug/agent delivering carries that have a long circulation time (few days) in the body and are permeable to cell membranes. Current choices are viruses and/or liposomes (vesicles comprised of natural phospholipids). However, most viruses cause side effects while liposomes are weak, environmentally sensitive and have a short circulation time. Over ten years of research and collaboration among multiple researchers at the University of Pennsylvania have led to the discovery of ...

A New Fast 2-D DCT/IDCT Algorithm and Its VLSI Implementation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:37 GMT

TechID: K1732 University of Pennsylvania researchers have developed an algorithm for calculating the 2-D 8X8 Discrete Cosine Transform (DCT) and Inverse Discrete Cosine Transform (IDCT) and have developed a method for VLSI (very large scale integration) implementation. Based on the direct 2-D matrix factorization approach, the proposed algorithm is not only computationally efficient and requires a smaller number of multiplications, but also has paralleled structure and localized interconnections. In addition, much shorter internal wordlength is required by the algorithm...

Functionalized Coatings on Metal Surfaces for Biomedical Implants

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:31 GMT

TechID: O2910 Metallic devices (stainless steel, titanium) are widely used for medical implants in a variety of organ systems from the skeletal system to cardiovascular. These materials are used because they are inert in the hostile environment of the body. However, they also have vastly different characteristics from the tissues which host these implants and, in the case of a hip joint for example, these differences can ultimately cause further damage or erosion of the hosting bone. Researchers at Penn and CHOP have developed a means of functionalizing these materials...

Initial Description of New Compounds and Their Precursors for Positron Emission Tomography of Hypoxia In Vivo

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:31 GMT

TechID: K1831 Many human and animal diseases are characterized by the pathological formation of tissue hypoxia and ischemia, and treatment of such conditions can only be optimized by determining the extent and degree of hypoxia in the affected tissues of individual patients. Positron emission tomography (PET) provides a desirable non-invasive detection method provided appropriate imaging drugs can be selectively delivered to the affected site. Researchers at the University of Pennsylvania have developed a scheme for the synthesis of a family of compounds, radiolabeled w...

Use of Ultrasound as an Antivascular Agent

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:29 GMT

TechID: S4128 Cancer uses the process of angiogenesis to rapidly form blood vessels to provide support for tumor growth. Pharmaceutical methods of anti-angiogenesis have been shown to shrink tumors and prevent cancer spread. Penn researchers have developed a non-HIFU[*] ultrasound technology to mechanically disrupt tumor neovasculature. This new method of anti-angiogenesis does not affect local stable human blood vessels but hinders tumor growth and cancer spread. Ablation of secondary metastatic tumors is a common palliative and quality of life i...

Spiral RF Coil for High Field MRI

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:28 GMT

TechID: K1842 The signal produced by magnetic resonance imaging devices increases with the strength of the magnetic field. At magnetic fields above 1.5 Tesla, the spatial variation of both the amplitude and phase of the field and the resulting signal will distort the images that are produced. As a result, the improved image quality of high-field devices will not be realized in many parts of the head or body. Researchers at the University of Pennsylvania have modified the design of a low-frequency coil for use at high frequencies. The modified design uses angled c...

Prostaglandin D2 Receptors in Cardiovascular Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:24 GMT

TechID: R3846 The withdrawal of Vioxx and Celebrex was due in large part to their inhibition of the production of protective prostaglandins in the arterial vasculature. Although effective in treating arthritis, these drugs induced substantial increases in the risk of major cardiovascular events. A more complete understanding of the atherogenic and atheroprotective roles for COX-derived prostaglandins is necessary for the development of anti-inflammatory drugs which avoid the adverse cardiovascular effects seen in the current generation of coxibs. Researchers at the Universit...

New Serotonin Transporter Imaging Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:23 GMT

TechID: L1993 Anxiety and depressive disorders have been associated with the level of serotonin in the brain. These disorders are typically treated by prescribing a selective serotonin reuptake inhibitor (SSRI). SSRI drugs (i.e., Prozac™) will bind with the serotonin receptor protein in the serotonin reuptake transporter (SERT) sites in the nuclei of the brainstem, effectively blocking the presynaptic transporter for serotonin. However, the efficacy of SSRI drug treatment is dependent on the ability of the drug to bind with the serotonin receptor protein. The effi...

New Therapeutic Agents for Tuberculosis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:18 GMT

TechID: H1277 Globally, tuberculosis (TB) in all of its manifestations is the leading cause of death from a single infectious agent. Studies from two urban centers indicate that 30-40% of new cases are the result of recent infection rather than reactivation of old disease, and cases acquired by recent transmission accounted for almost two-thirds of drug resistant TB. Analysis of the microbial regulation of DNA replication—typically a target for new antimicrobial agents—indicates that a promising mode for drug action is the inhibition of ribonucleotide reductase (RR), an...

Intraoperative Beta-Probe

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:15 GMT

TechID: I1410 Although devices such as scintillators can be used to detect radiation, for example, beta-radiation-emitting tracers in tumors, they have met with limited success due to their sensitivity to background gamma radiation. Since most beta-emitting isotopes also have gamma emissions, a detector that is sensitive to gamma rays is likely to be affected by background gamma radiation and lead to ambiguity in the location of the tumor. Existing beta-sensitive probes are limited in the face of metastasis from a s...

Endorectal Surface Coil For Female Pelvis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:11 GMT

TechID: D769 Insertable intracavity probe for use in enhanced magnetic resonance imaging of a body cavety, specifically the colon. The probe consists of an elongate shaft with a handle at its proximal end and an inflatable balloon structure at its distal end containing a loop-type pickup coil for connection to an interfacing network. When inserted in a body cavity, the balloon structure is positioned adjacent to an area of interest, and the inflation and deflation of the balloon allows for optimal positioning of coil near the area of interest. Applications High quality magneti...

Integration of Optical Flow and Deformable Models

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:07 GMT

TechID: J1489 Computer face tracking and expression recognition has received intense interest in recent years due to their applications in human-computer interfaces. University of Pennsylvania researchers have developed a deformable face model that predicts and follows facial motion in successive computer images. Deformable models are shapes specified by a set of parameters that deform by the application of forces defined within a physical model. By analyzing the changes in image intensity around areas of high curvature, e.g. checks and nose, forces are determined...

Endothelial Cell Specific Gene Expression Using Viral LTR

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:27:01 GMT

TechID: G1105 The long terminal repeats of the retroviral genome function as promoter/enhancer elements as well as polyadenylation signals. University of Pennsylvania researchers have developed a method for gene therapy specific to human endothelial cells using modified retroviral long terminal repeats. Non-retroviral human protein gene sequences have been operably linked to the long terminal repeats for gene expression and protein production exclusively in endothelial cells. Applications Advantages

Aromatic Peptidomimetics

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:58 GMT

TechID: E904 Peptides are important in many biochemical processes in humans. Two classes of peptides, hormones and neurotransmitters, are controlled by receptor-mediated stimulation of regulatory proteins called G proteins. Examples of G protein-linked receptors include receptors for somatostatin, catecholamines and substance P. Synthetic peptides could mimic or inhibit biological and/or chemical activity of the peptides that bind G protein-linked receptors. However, limited therapeutic applications exist involving peptides due to poor oral bioavailability a...

Novel Growth Factors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:56 GMT

TechID: D712 Myelin destruction of nerve cells is the main pathology of demyelinating neuropathies, such as multiple sclerosis. The binding of a viral receptor expressed on oligodendrocytes stimulates the appearance of components of myelin biosynthesis, laying the groundwork for earlier remyelination of damaged nerves. University of Pennsylvania researchers have developed a method to treat mammalian diseases and conditions characterized by myelin destruction by administration of an antibody or synthetic peptide which specifically binds this viral receptor and stimulates...

Fluorophore Concentration and Lifetime Images with Diffuse Light

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:52 GMT

TechID: J1556 Diffusing near IR light has received intense interest as a new medical imaging technique to non-invasively image heterogeneities, i.e. tumors, in biological tissue. Recent developments include the addition of fluorescence based contrast agents with diffusing IR light as a means to enhance the specificity and sensitivity of tumor detection. The basis for this method is the accumulation of fluorophores and the variation of the fluorophore lifetime at tumor sites. University of Pennsylvania researchers have developed analytic techniques to calculate the behav...

Use of a Human Transposable Element in Gene Delivery and Mutagenesis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:51 GMT

TechID: I1346 Transposable elements (transposons) are a class of DNA sequences that can move from one chromosomal site to another. Transposon mutagenesis has been a valuable tool in identifying and cloning genes in several species such as Drosophila and various plants. University of Pennsylvania researchers have identified a transposon mutagenesis system that can be used in mammalian cells. Transposons have been identified that are related to the genes associated with human genetic disorders and tumor suppression. The mutant phenotype usually results from the inse...

Visually Intuitive Monitoring

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:42 GMT

TechID: U4672 Health care practitioners are working with an ever-growing volume of electronic patient information without optimal means of navigating and displaying relevant data. MedDash Medical Dashboards address these issues with a proprietary software system built around an innovative data representation concept known as Visually Intuitive Monitoring or VIM. VIM provides a simple user interface to allow rapid, lag-free browsing of all available electronic patient data. While data are currently available mostly in a raw, unorganized fashion, VIM presents information using vis...

Polyclonal Antibodies for Rat 3a-hydroxysteroid Dehydrogenase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:41 GMT

TechID: M2291 The enzyme, 3a-hydroxysteroid/dihydrodiol dehydrogenase (AKR1C9), is widely distributed in rat liver, lung, testes, and small intestine. This enzyme has a variety of actions such as steroid hormone and carcinogen metabolism. Although some researchers report multiple forms of this enzyme, University of Pennsylvania researchers have recently shown only a single isoenzyme through comprehensive experiments in rats. Using their purified homogenous enzyme from rat as antigen, they raised high-titer poly...

Endometriosis Mouse Model

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:41 GMT

TechID: J1594 Please call licensing director for more information Applications Advantages

Monoclonal Antibodies against Poxvirus Extracellular Enveloped Virus (EEV) and Intracellular Matrix Virus (IMV)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:37 GMT

TechID: Q3560 Mice monoclonal Abs against VV proteins B5R, L1R and A33R protect mice against IMV and EEV vaccinia challenges. Applications Poxvirus antibodies Advantages See Technology Summary

Graphical information display for intensive care

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:36 GMT

TechID: I1424 University of Pennsylvania researchers have developed a method and apparatus for displaying information about the medical condition of a patient in the surgical intensive care unit. This interactive information processing system includes steps for gathering the information from multiple measuring instruments, calculating derived patient values from this information, and displaying these derived values in graphical format. The system can also be used in a "Virtual Therapy" capability which allows the user to model various treatment scenarios and their effec...

Standardizing MR Image Intensity Scales

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:33 GMT

TechID: K1768 Magnetic resonance imaging (MRI) is being increasingly used as a diagnostic tool in radiology. A drawback of MRI images is the lack of a standard and quantifiable intensity scale. The image intensity is dependent on both the scanner and patient. Contrast and brightness adjustments are required to view different images of the same patient. In addition, images taken for the same patient at different times or on different scanners may have different intensities. As a result, it has been a major challenge to obtain quantitative information about hea...

Glycogen Synthase Kinase-3 as a Therapeutic Target

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:26 GMT

TechID: I1456 University of Pennsylvania researchers have developed a method to identify compounds that mimic the therapeutic effects of lithium. These researchers have found that lithium works by potently inhibiting a kinase that regulates cell fate determination in a variety of organisms. However, it is not a general inhibitor of other protein kinases. These results were determined by in vitro and in vivo assays, which can also be used to screen new compounds for their ability to inhibit this same protein kinase and thus mimic the activity of lithium. A test co...

Design and Synthesis of Novel Breast Cancer Chemotherapeutic Agents Based on the Antimitotic (+)-Discodermolide

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:23 GMT

TechID: K1885 Discodermolide, a metabolite of a deep water marine sponge, holds great promise as a chemotherapeutic agent for breast cancer. Recent studies have shown discodermolide to be one of the most potent antimitotic agents known to date. It has been found to possess mechanisms of action similar to that of TaxolTM, while being 160-fold more water-soluble than TaxolTM. Discodermolide was also shown to be fully active against multidrug-resistant human ovarian carcinoma cells, which were 20-30 fold more resistant to TaxolTM than the parental line (Molecular Pha...

Biologically Active Peptides and Methods of Identifying, Constructing and Using the Same

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:19 GMT

TechID: F980 Protein-protein interactions are important in many biological functions, including the immunological response to pathogenic antigens. These interactions include a protein or pathogen binding to a receptor, protein interactions at cellular attachment sites, and adhesion protein interactions. Peptides or proteins that mimic or block these protein-protein interactions can be valuable assets to alter cellular function or to prevent infection. University of Pennsylvania researchers have developed a method to identify the amino acid sequence of pathogens that ser...

Development of CD4-Independent HIV Envelope Proteins as a Novel Vaccine and Therapeutic Modality

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:17 GMT

TechID: L1946 The interaction of the Envelope glycoprotein of HIV with the cellular receptor CD4 triggers the exposure of a chemokine receptor binding site in Env that permits the interaction of Env with a chemokine receptor which subsequently leads to viral fusion and entry. The recently solved crystal structure of Env has demonstrated that this chemokine receptor binding site on Env is a highly conserved region that is normally hidden in the intact Env structure. It is predicted that antibodies to this site might be highly cross-reactive and broadly cross-neutralizing, and contri...

A Transgenic Mouse Model for Macular Degeneration

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:17 GMT

TechID: J1604 Macular degenerations are a group of distinct disorders characterized by retinal degeneration. This loss of retinal cells results in loss of central vision and fine visual discrimination. Previously, pathogenesis of macular degenerations has been difficult to study because of the lack of spontaneously-occurring animal models. However, the study of one form of macular dystrophy in particular, Sorsby’s fundus dystrophy (SFD), has begun to shed new information on this disorder. SFD is an autosomal dominant macular disorder with the onset of symptoms in the third or ...

Only a Portion of a microRNA is Required for Target RNA Recognition and Cleavage

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:13 GMT

TechID: Q3313 RNA interference is a phenomenon observed in eukaryotic cells in which very short RNA molecules hybridize to a much larger mRNA target and cause the destruction of the mRNA. This phenomenon has been exploited by researchers and adopted as a powerful research tool, and has led to the development of siRNA molecules as gene directed therapeutics. Researchers at the University of Pennsylvania have examined the process of RNA interference in depth, and have established a good understanding of the cellular processes required for RNAi to occur, identifying and ch...

Aggregation of Alpha-Synuclein in Lewy Bodies of Sporadic Parkinson's Disease and Dementia with Lewy Bodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:12 GMT

TechID: K1828 Lewy bodies are considered the hallmark or diagnostic lesion of Parkinson’s disease. Related lesions also occur commonly in the brains of patients with Alzheimer’s disease and a late-life neurodegenerative disorder, known as dementia with Lewy bodies. Researchers at the University of Pennsylvania have raised monoclonal antibodies against a specific protein from the Lewy lesions. One of the monoclonal antibodies has been found to react specifically to alpha synuclein protein, which is found in Lewy bodies from Parkinson's disease, Alzheimer's disease or dementia a...

Method for Identifying Steroid Unresponsive Patients Prior to, or During Therapy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:26:07 GMT

TechID: N2553 Many diseases (e.g., RA, IBD, systemic lupus erythematosis, asthma) that are primarily inflammatory, or that have a major inflammatory component, are treated with steroids. In addition, some cancers are treated with steroids, as are transplant recipients. However, the effectiveness of steroid treatment varies and is usually impossible to predict. Some patients may be constitutively non-responsive to a particular medication, and others may become refractory to treatment over time. Consequently, the inclination to continue steroid therapy and eve...

Treatment of Acute and Chronic Lung Disease and Prevention of Aging using Flaxseed and its Active Ingredients

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:56 GMT

TechID: Q3337 Flaxseed has high contents of omega-3 fatty acids and lignans with antioxidant properties. This invention is directed to methods and compositions for treating pathological lung conditions using whole-grain flaxseed or flaxseed lignans (isolated in form of the lignan precursor Secoisolariciresinol diglucoside (SDG) directly from whole-grain flaxseed or chemically synthesized directly into the mammalian, readily metabolizable forms, Enterodiol (ED) or Enterolactone (EL). Such pathological conditions result from oxidative lung injury such as ischemia-reperfusion inju...

Method and Apparatus for Restructuring Data

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:56 GMT

TechID: K1851 Efficient data access has become increasingly more important due to expanded information availability from sources such as the Internet. Relational databases provide convenient features for accessing large amounts of data, but they require prior knowledge of the type of data to be stored and have difficulty accounting for additional data and their relationships to existing data. On the other hand, semi-structured databases do not require such prior knowledge, but they exhibit shortcomings in efficient data ac...

Metal Complexes as Protein Kinase Inhibitors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:52 GMT

TechID: P3200 Since the mid-1990s there has been a near exponential rise in the level of protein kinase and glycogen synthase kinase-3 (GSK-3) directed research. Consequently the therapeutic potential of protein kinase inhibitors in general and GSK-3 inhibitors in particular has become a rapidly growing area of interest to the pharmaceutical industry. There is particularly strong evidence to support the development of GSK-3 inhibitors as: 1) inhibitors of neuronal apoptosis and neurological decline in stroke patients; 2) therapeutics targeted to bipolar disorder and depression; ...

Development of highly sensitive Luciferase-based alternative splicing reporter assay for use in high throughput screens for regulatory proteins and compounds that regulate splicing

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:50 GMT

TechID: U4755 Our team has developed a series of fluorescence- and luciferase-based splicing reporter constructs that can be used to carry out high throughput functional assays for proteins or compounds that influence specific alternative splicing pathways ((Hovhannisyan et al., 2006; Newman et al., 2006)and submitted). The minigenes used in these assays were designed in a manner in which fluorescent or luciferase fusion proteins are encoded by a construct that contains introns and one or more exons whose inclusion or skipping determines a reading frame that will or will no...

Use of BBI/BBIC for the Treatment of Autoimmune Diseases Such as Multiple Sclerosis or Guillain Barre Syndrome

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:49 GMT

TechID: N2380 Serine proteases are associated with the induction of autoimmune and neuro-inflammatory disorders. Researchers at the University of Pennsylvania have identified a novel use for a serine protease inhibitor as an agent to suppress several neuro-inflammatory diseases. Studies in animal disease models show profound effects of this compound on neuro-inflammatory disorders. The equivalent disabling neuro-inflammatory diseases found in humans affect a large number of adults worldwide. Treatments for these diseases, which currently consist of daily injections of antibody, have demon...

A Peptide Inhibitor for G-Protein-Activated Potassium Channels

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:46 GMT

TechID: K1726 Patented Peptide Inhibitor of Inward-Rectifier K+ Channels Tertiapin-Q Introducing Dr. Zhe Lu’s potent and specific inhibitor of G-protein gated inward-rectifier K+ channels: Tertiapin A L C N C N R I I I P H M C W K K C G K K Tertiapin-Q A L C N C N R I I I P H Q C W K K C G K K Relevance: Inward-rectifier K+ channels are a group of highly specialized potassium channels that differ structurally as well as functionally from voltage-activated K+ channels. Until now, high-affinity ligan...

Chiral Polyaniline Materials

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:44 GMT

TechID: J1630 Polyanilines are well known conductive polymers. The present invention affords a method for the preparation of chiral polyanilines by incorporating chiral dopants into the polymerization. The chiral polyanilines have properties which extend their utility into novel areas. Applications Advantages

Repopulation of Testicular Seminiferous Tubules with Foreign Cells, Corresponding Resultant Germ Cells, and Corresponding Resultant Animals and Progeny

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:43 GMT

TechID: E901 A method has been developed to use sperm "stem" cells to transfer genetic modifications to an animal’s progeny. This technology was developed by a University of Pennsylvania researcher who previously developed the first transgenic mouse. Sperm "stem" cells (which give rise to sperm) have been successfully transferred from the testicles of one mouse to another, resulting in the production of normal offspring. Successful transferal experiments have been performed in mice and rats; concentrated work remains on the cultivation and alteration of sperm stem...

Intracellular Delivery of Macromolecules

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:38 GMT

TechID: I1354 Issued Claim 1 of 38: A composition comprising a liposome consisting essentially of a plurality of vesicle-forming lipids, a bacterial hemolysin, and a therapeutic agent or diagnostic agent, wherein said bacterial hemolysin and said therapeutic agent or diagnostic agent are encapsulated within the lumen of said liposome. Patent: US 5,643,599 Applications Advantages

A General Approach to the Design of Type I Ribonucleotide Reductase Inhibitors (Combined into K1767)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:38 GMT

TechID: H1296 Ribonucleotide reductase reduces ribonucleotide diphosphates to 2'deoxynucleotide diphosphates for DNA synthesis. Enzymatic activity requires the association of four subunits into a ?2?2 structure. Blocking this association is a well-recognized approach to the rational design of anti-cancer and anti-viral therapeutics. Expression of the enzyme is tightly coupled to the S phase of the cell cycle, so that inhibitors of the enzyme only inhibit dividing cells or replicating viruses. A variety of molecules with activity as inhibitors of ribon...

Novel Therapeutic Agents for the Treatment of Asthma

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:35 GMT

TechID: G1161 Asthma is a disease characterized by airway inflammation and hyperresponsiveness, bronchial smooth muscle contraction and, in severe cases, bronchial smooth muscle hyperplasia. Cytosolic calcium release in human airway smooth muscle cells is required for small muscle contraction and stimulation of human airway muscle cell proliferation. University of Pennsylvania researchers have developed a novel technique to inhibit cytosolic calcium release in smooth muscle cells of human airways. The methods and compositons of this invention provide an alternativ...

Novel Inhibitor of Tissue Necrosis Factor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:32 GMT

TechID: J1573 xTumor Necrosis Factor (TNF) an inflammatory mediator, has been associated with a number of pathological conditions. The current use of anti-TNF antibody and soluble TNF-R to inhibit TNF has shown limited success. Researchers of the University of Pennsylvania have improved therapeutic options by designing effective and selective TNF antagonists, specifically, inhibitors of TNF-alpha. These peptides and peptide analogs mimic the functions of soluble TNF-R, thus overcoming limitations of macromolecular compounds. In addition, the technique for drug design can be used to...

Monoclonal Antibodies to DC-SIGN and DC-SIGNR

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:29 GMT

TechID: P2971 A variety of cell surface molecules can support virus attachment and increase the efficiency of viral infection. DC-SIGN, a membrane protein expressed on immature dendritic cells (DCs) and certain macrophages, can bind to HIV-1, HIV-2 as well as simian immunodeficiency virus (SIV), and present these viruses to receptor-positive cells, facilitating HIV transmission. Therefore, it is important to further characterize the expression patterns of DC-SIGN and the mechanisms by which DC-SIGN binds and transmits virus. University of Pennsylvania researchers have ...

A Strategy for Identifying Compounds that Block DNA Synthesis of Tumorigenic KSHV/HHV-8

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:29 GMT

TechID: K1804 Kaposi's sarcoma-associated herpes virus (KSHV), or HHV-8, has been implicated in several human cancers such as Kaposi’s sarcoma, the most common neoplasm of AIDS patients. KSHV is also strongly associated with certain lymphomas, especially multiple myeloma, a form of bone cancer. In order to develop an anti-viral strategy and to screen chemical compounds for their potential to perturb DNA synthesis and replication of KSHV, researchers at the University of Pennsylvania have described a convenient colorimetric micro...

Immortal Cell Lines that are Deficient in Bax and Bak

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:24 GMT

TechID: O2707 Programmed cell death, known as apoptosis, plays a critical role in development and disease, and may be triggered through an extrinsic pathway initiated through ligand binding to cell surface receptors, or an intrinsic pathway initiated within the cell. Current assays used to screen drugs for toxicity or protection from cell death do so without identifying the underlying pathway involved, and may not easily differentiate the toxic side effects of drugs that are independent of their ability to induce apoptosis. Researchers at the University of Penns...

Method for Assaying Histone Deacetylase Activity

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:16 GMT

TechID: O2814 Histone proteins associate with DNA in chromatin and regulate gene transcription activity. Histone deacetylases mediate gene transcriptional repression by deacetylating histones. It is known that histone deacetylases interact with tumor suppressors and oncogenes, playing a role in the pathogenesis of many human cancers. Inhibitors of histone deacetylases, such as sodium butyrate, are useful therapeutic agents in cancer treatment. University of Pennsylvania researchers have developed a novel histone deacetylase continuous activity assay. ...

A Fluorescence Method for Immuno-Detection of Epitopes on Molecules and for Detection of Protein-Protein Interactions

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:15 GMT

TechID: N2492 CD4 is a transmembrane glycoprotein that functions as the major T-cell receptor for HIV-1. The CD4 receptor binds to the HIV-1 envelope protein GP120 with very high affinity. This binding is the first step in viral infection and inhibition of this step is critical for developing therapeutic methods to inhibit the disease. The three dimensional structure of the CD4 receptor was recently determined. University of Pennsylvania researchers have used computer modeling to develop constrained analogs of the CD4 receptor in order to block viral binding. These analogs can bind...

PC6-3 Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:14 GMT

TechID: M2311 A fundamental knowledge of genes and proteins that control cell death is important for understanding developmental events, normal aging process, and disease pathophysiology. Understanding the mechanisms responsible for neuronal cell death requires model systems for identifying the molecules and pathways involved in this process. Researchers at the University have generated a novel cell-line PC3-6 that was generated from the parent cell-line PC12, a common but also very heterogeneous cell line used by many labs.&nbs...

Analysis of Trabecular Microstructure by MR Microscopic Imaging in vitro and in vivo

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:11 GMT

TechID: D845 Osteoporosis is a disorder affecting up to 25% of the elderly female population. Most osteoporotic fractures occur at skeletal locations rich in cancellous (trabecular) bone. . Current methods of assessing trabecular bone structure are based on histomorphometry, requiring a bone biopsy, which is an invasive procedure. A noninvasive method for characterizing the trabecular microstructure would be useful for assessing the severity of osteoporosis and for diagnosing patients at risk, as well as for monitoring the effect of treatment. Magnetic reso...

New Animal Models of Human Esophagus Cancer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:10 GMT

TechID: S4147 Mouse model of esophageal cancer, research tool. Transformed human esophageal epithelial cells were injected into mice. Squamous carcinomas were mimicked by c-Myc & Bcl-XL transformants, and adenocarcinomas by H-Ras, EGFR, and Akt transformants. Useful for research understanding of esophageal cancer, and of potential value as a screening tool for leads. Applications Mouse model of esophageal cancer, or transformed esophageal epithelial cell which can be injected into Balb mice to stimulate development of specific tumor types with specific gene expression profiles.&n...

Anti-DNA scFv as Intracellular Delivery Vehicles for Proteins, DNA and RNA

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:08 GMT

TechID: K1771 Work on developing an efficient carrier system for transporting materials into the nucleus of living cells has received intense interest in recent years due to its application in gene therapy. Current methodologies make use of viral vectors for transport, yet these vectors create risk of immunogenic reactions and lack efficiency and stability. University of Pennsylvania researchers have discovered an efficient carrier system for transporting proteins, DNA and/or RNA into the nucleus of living cells. This novel approach uses recombinantly expressed si...

Anti-UXT Monoclonal Antibodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:03 GMT

TechID: S4122 Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Penn researchers have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues, that UXT is located in human centrosomes and is associated with -tubulin, that overexpression of UXT disrupts centrosome structure, and that abrogation of UXT protein expression by small interfering RNA knockdown leads to cell death. To determine the localization of endogenous UXT molecules and to assess the contributi...

A Susceptibility Gene for Idiopathic Epilepsy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:25:00 GMT

TechID: N2383 Introducing the first genetic test able to detect risk associated with the development of common types of epilepsy: *Table 2 Comparison of allele distribution by Mantel-Haenszel chi square contingency analysis _____________________________________________________________________ Comparison &n...

Inverse Kinematics

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:59 GMT

TechID: C.00-95 Inverse Kinematics using ANalytical Methods IKAN uses a combination of analytic and numerical methods to solve generalized inverse kinematics problems including position, orientation, and aiming constraints. The combination of analytic and numerical methods results in faster and more reliable algorithms than conventioal inverse jacobian and optimization based techniques. IKAN allows the user to interactively explore all possible solutions using an intuitive set of parameters that define the redundancy of the system. Applications IKAN is a complete set of inve...

Dermal Fillers to Thicken Myocardial Infarctions

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:54 GMT

TechID: T4522 Five million Americans suffer from heart failure and 250,000 die from the disease each year. Approximately 70% of all heart failure cases occur as a result of a myocardial infarction (heart attack). In most cases patients who survive a myocardial infarction are initially asymptomatic, however, many develop progressive heart enlargement, loss of heart function and symptoms of heart failure over the ensuing months and years after their initial heart attack. This progressive process is termed ventricular remodeling. In spite of modern medical and surgical treatment th...

EIN2 Gene and Mutations

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:47 GMT

TechID: G1190 The earliest currently detectable event to occur during plant-pathogen interaction is a rapid increase in ethylene biosynthesis. Ethylene, one of five well-established plant hormones, mediates fruit ripening, leaf abscission, and flower senescence. The production of ethylene is induced by stress conditions. Plants which tolerate the growth of a pathogen without sustaining damage are beneficial. University of Pennsylvania researchers have isolated an ethylene insensitivity mutant, ein, and a hookless mutant, hls1. Ein mutations result in e...

SIH for Protection Against Retinal Degeneration

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:45 GMT

TechID: S4273 This invention is related to methods for treating or preventing age-related macular degeneration, blindness or glaucoma using an iron-chelator or SIH. Iron is known to cause oxidative stress. Elevated iron levels are found in the retinas of patients with age-related macular degeneration, suggesting that iron may play a role in the pathogenesis of AMD. Previous studies have demonstrated the efficacy of certain chelators in reducing iron levels in various cell types, including RPE cells; it has also been shown that iron chelation confers cytoprotection against oxidat...

Expression of a Dominant Negative Neuropilin-1 Disrupts Olfactory Axon Guidance In Vivo (Combined into M2150)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:45 GMT

TechID: M2226 In the developing nervous system, the growing tips of axons detect and respond to a multitude of attractive and repellent environmental signals. The semaphorin family of signaling proteins, including SEMA-3A, SEMA-3C and SEMA-3F, are thought to act as repellent guidances for a variety of specific axons. Neuropilins have recently been characterized as receptors for these semaphorins. Researchers at the University of Pennsylvania have recently generated neuropilin-1 deletion constructs to explore its functional role in semaphorin receptors. Deletion of a port...

Trans-bronchial SVO2 Monitor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:43 GMT

TechID: O2651 TRANS-BRONCHIAL SATURATED VENOUS OXYGEN MONITOR. Introduction: In anesthesia and critical care medicine, prevention of tissue hypoxia is vital. Mixed venous oxygen saturation of blood in the pulmonary artery has emerged as an important monitor of the balance between oxygen delivery and oxygen demand, and specialized pulmonary artery catheters can provide this important information. However, these invasive monitors have a high potential for serious complications. Hence their use is often delayed or forgone altogether. This invention aims t...

Use of Listeria as a Live Cancer Vaccine

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:39 GMT

TechID: H1219 The immune system faces many unique challenges when trying to rid a body of cancerous cells. One problem is that cancer cells down regulate MHC class I expression as well as the B7 molecule which are both required for the activation of T lymphocytes. A second problem is that antigens must be identified that are specific to cancerous cells in order to distinguish themselves from normal cells. Researchers from the University of Pennsylvania have overcome these hurdles in vaccine therapy development and found a feasible and potent live vaccine for cancer usin...

Novel Plasma-Derived Molecular Signatures from Pulmonary Arterial Hypertension Patients

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:37 GMT

TechID: U4593 PAH is defined by the presence of abnormally elevated pressures in the pulmonary vasculature. Without treatment, the median survival age for PAH patients is 2.8 years from diagnosis. At the level of the pulmonary arterial wall, sustained vasoconstriction and remodeling of pulmonary arteries occur in various forms of PAH. Recent clinical and pre-clinical studies have demonstrated that the genetic and environmental basis for PAH is both multi-factorial and unique for each patient. There is a lack of diagnostic and therapeutic options for these patients, which leaves the...

New Immune Cell Receptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:36 GMT

TechID: O2699 Recent research has revealed details on the importance of the innate immune system for recognizing and eliminating tumors. The natural killer cell receptor NKG2d contributes to this immunity by binding to MHC class I molecules and to stress activated ligands that are elevated in potentially cancerous cells. NKG2d expressing immune cells are then believed to mediate cytotoxic effects to remove tumor cells as well as trigger an immune response against the cancer. Researchers at the University of Pennsylvania have identified and cloned a novel C-type ...

Bioactive and Degradable Composite Microspheres

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:36 GMT

TechID: L2064 Traditional microspheres used in 3-D cell cultures and drug delivery are primarily made from biocompatible or degradable polymers. Researchers at the University of Pennsylvania have invented a bioactive and degradable composite microsphere for tissue engineering and regeneration. The composite microspheres can form a chemical bonding with living bone tissue without a fibrous tissue at the interface, and degrade with time to allow the tissue replacement. The process developed for the composite microsphere synthesis allows the incorporation of active biological molecule...

SDF-1a for the Treatment of Chronic Skin Wounds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:32 GMT

TechID: S4256 Omaida Velazquez and Katherine Gallagher have shown that Stromal-derived-factor 1 alpha (SDF- 1a) is a chemokine that promotes wound healing in chronic skin wounds in conjunction with treatment with Hyperbaric Oxygen Therapy using a diabetic mouse model. SDF-1a functions as a chemo-attractant guiding bone marrow-derived Endothelial Progenitor Cells (EPCs) to the area of the wound. The use of Hyperbaric Oxygen Therapy has been shown in previous studies, to increase the number of EPCs in circulation. The data described in the invention (#60/907...

Modified Avidin -BioTin Technique

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:31 GMT

TechID: E965 In indirect enzyme-linked immunohistochemical procedures, the secondary antibody serves the pivotal role of linking the primary antibody bound to the antigen of interest in a tissue section with a tertiary complex containing an enzyme reporter molecule. One of the most common methods for this is the avidin-biotin-complex (ABC) method. This method is simple, has high signal amplification, and low background noise. These features of the ABC method have prompted its widespread use as an immunohistochemical detection system. There is, however, one major ...

Microemboli Mouse Model

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:29 GMT

TechID: M2158 Red blood cells (RBC) are the most abundant cellular constituent of the blood, have a life span of 100-120 days and travel ~250 km through the cardiovascular system before they are cleared as a result of aging. Thus, RBC have several features that may enable them to serve as safe and abundant drug carriers. The drugs can be loaded into RBC or coupled to their plasma membrane. The resultant drug/RBC complexes may result in having prolonged circulation in the bloodstream and limited filtration into tissues, features that may improve the therapeutic pro...

ECG Gated Ventilation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:24 GMT

TechID: D829 A recurrent problem in the medical and surgical intensive care field is the patient with coexistent respiratory and cardiac failure. Since cardiac and ventilator devices are not synchronized to work with each other, augmenting one system may actually diminish the effectiveness of the other system. To prevent this problem, University of Pennsylvania researchers have developed a non-invasive device for improved treatment of patients on ventilators. This device, called the gated programmable ventilator, simultaneously monitors the patient’s electrocardio...

Regulation of TLR Signaling by Complement

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:22 GMT

TechID: S4250 Toll-like receptors (TLRs) are components of the innate immune response, functioning as molecular sensors of microbial infection. TLRs can be activated by various pathogen-derived constituents such as LPS and bacterial / viral RNAs and DNAs. Activation of TLRs leads to priming of the adaptive immune system, ultimately resulting in antigen-specific and protective T + B cell immunity. Dr. Song’s group discovered that TLR-mediated effects leading to T + B cell immunity can be triggered by complement activators (e.g. cobra venom factor) and activated complement products ...

Bacterial Bound Airway CRP for the Diagnosis of Respiratory Tract Infections

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:15 GMT

TechID: P2930 Respiratory tract infections are difficult to diagnose and there is little diagnostic progress in the last fifty years. The current diagnosis of respiratory tract infections in adult patients relies on microbiological laboratory techniques including sputum gram stain, sputum culture and blood culture, and is further limited to blood culture for children. Because of the heavy presence of colonizing microorganisms in the human upper airway, sputum gram stains and cultures lack specificity and often fail to help the identification of the particular microorgan...

Biocompatible ECM Model for Adult Stem Cell Applications with Widely Tunable Elasticity (Combined into S4048)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:10 GMT

TechID: T4307 Several scientists recently have dealt with issues such as how substrate stiffness regulates cell traction forces or ways stem cells can differentiate based on cell shape, but no literature seems to explicitly relate matrix elasticity with stem cell differentiation. Researchers at Penn here present an enhanced method of modifying and cross-linking hyaluronic acid that allows for a well-defined effective Young’s modulus over a wide range (0.1 - 120 kPa). This biocompatible hydrogel can be used as supporting matrix for adult stem cell culturing, expansion and di...

Diffuse Photon Density Waves for Object Location with and without Fluorescent Contrast Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:09 GMT

TechID: G1112 Traditional medical imaging techniques used in CT, MRI, or PET scanning reconstruct an image based on the propagation of unscattered radiation. Researchers at the University of Pennsylvania have invented an imaging system which reconstructs images from multiply scattered diffuse light. The system consists of a source of near infrared light for generating oscillatory photon density waves to illuminate an object, a detector for detecting diffuse photon density waves, and computer processors interacting with the detectors for data processing. Diffuse photon waves refract...

Red Blood Cell Agglutination Assays Using Anti-Red Cell Phage-Displayed Antibodies and Anti-Phage Antibodies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:04 GMT

TechID: J1500 This technology, developed at the University of Pennsylvania, comprises an adaptation of, and improvement to, a commercially available cell panning methodology. In addition to facilitating red blood cell antigen typing, this invention adapts the conventional indirect agglutination test by adding a specific non-human phage anti-antibody. Thus, this novel invention is more effective for inducing agglutination than present methods using anti-human IgG. Applications Advantages

B1B5

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:24:02 GMT

TechID: P2989 Researchers at the University of Pennsylvania have developed a mouse monoclonal antibody, IgG2b, which specifically recognizes the platelet glycoprotein IIb, which together with glycoprotein IIIa, forms the human platelet fibrinogen receptor. This monoclonal antibody reacts with IIb by western blot and immunoprecipitation. Therefore, the mouse monoclonal antibody may be useful in a variety of research settings as well as in the development of platelet-inhibiting pharmacological agents. Applications This antibody may be useful in a variety of research setting...

Non-Steroidal Mechanism Based Inactivators of Steroid and Prostaglandin Transforming Enzymes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:59 GMT

TechID: D572-C-02 Diseases of the prostate are prevalent among adult men. Fifty-percent of men above the age of 65 will experience benign prostatic hyperplasia (BPH). The prostatic carcinoma (PCA) is the second leading cause of cancer death in men. Both diseases are androgen dependent and are treated with androgen ablative therapy. This technology developed by researchers at the University of Pennsylvania is comprised of small molecule non-steroidal inhibitors that will prevent the formation of active androgen and therefore will decrease androgen level...

HSV-1 Glycoprotein B(gB) - Monoclonal Antibodies and Expression System

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:56 GMT

TechID: O2887 The envelop of herpes simplex virus (HSV) contains at least 11 virus-encoded glycoproteins. Glycoproteins B (gB) is required for virus infection of host cells. The vast majority of gB is exposed on the outside of the virion and is a target of several virus-neutralizing antibodies. University of Pennsylvania researchers have developed monoclonal antibodies against HSV-1 glycoprotein B and an expression system to produce these antibodies (as referenced in S. Samanta et al., 19th International Herpesvirus Workshop, Ab.#29, 1994). In addition, they have employed a baculo...

123.123231Small Molecule GPVI Antagonists

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:48 GMT

TechID: R3789 Integrin a2ß1 [GPIa-IIa] Small Molecule Inhibitors Anti-Thrombotic Agents Introducing William F. DeGrado’s latest development: highly selective a2ß1 antagonisists. Relevance: Integrin a2ß1 is a widely expressed and unique alpha-subunit containing (I)-domain integrin that selectively binds to type I, type II-V collagens, and laminins 1&5. Unlike other receptors, a2ß1 has multiple conformation states that are regulated by intracellular signaling pathways. Integrin a2ß1 plays a critical role in platelet-mediated hemostasis and thrombosis, cancer metastasis,...

Importance of the Mouse Model in Glaucoma Research

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:47 GMT

TechID: N2465 Glaucoma affects an estimated three million people in the U.S and it is a major cause of human blindness and is usually associated with an elevation of pressure in the eye (the intraocular pressure or IOP). The pressure within the eye reflects a balance between the rate of formation of fluid (the aqueous humor) and the rate of fluid exit. Monitoring and measuring IOP is crucial for the treatment of glaucoma. University of Pennsylvania researchers have developed a reliable, reproducible technique called the servo-null micropipette system (SNMS) for measuring the hydros...

Electronic Nose

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:45 GMT

TechID: R3766 Carbon nanotubes are ideal candidates for sensing applications owing to their high aspect ratios, large surface areas, unique thermal and electronic properties. Furthermore, carbon nanotube based sensors are several orders of magnitude smaller than traditional micro-electromechanical (MEMS) based sensors, therefore are much more sensitive and less power consuming. Researchers at the University of Pennsylvania have tapped on these distinctive properties of single walled carbon nanotubes (SWNTs) and fabricated functionalized SWNT sensors targeting species that remain ...

The Use of Gamma-Interferon to Delay/Prevent Breast Cancer in Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:42 GMT

TechID: M2339 Gamma-interferon, secreted by thymus-derived cells and natural killer cells, plays a complex role in conferring resistance in humans to pathogens. Recently, a correlation between the lack of gamma-interferon and tumor incidence and latency has been observed in mice. While this may reflect alterations in immune surveillance, the mechanism of this effect is unknown. Mouse models have been shown to be powerful research tools for understanding disease in humans. Researchers at the University of Pennsylvania have developed mi...

M9 Peptide and Derivatives Thereof

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:39 GMT

TechID: M2315 Adenoviral vectors are important tools for gene transfer in gene therapy, but their uses can be limited by complications. Nonviral gene transfer methods, such as lipofection, are desirable alternatives. However, lipofection of nondividing cells is inefficient because much of the transfected DNA is retained in endosomes and that which escapes to the cytoplasm enters the nucleus at low rates. To improve the efficiency of nonviral gene transfer, University of Pennsylvania researchers have developed a novel transfection method using a peptide and its derivati...

Electrically Induced Osteogenesis in the Spine

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:39 GMT

TechID: O2634 It has been shown that electric signal application to a pair of surface electrodes placed on the skin overlying a bone defect and certain fractures can result in healing of the bone. This technology has also been successful as an adjunct to the treatment of localized spine fusion. To date, there has been no animal or human data for application of electric signals to aid spine fusion at multiple levels. University of Pennsylvania researchers have addressed this need by creating a device for ...

Novel method for expansion of regulatory T cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:36 GMT

TechID: U4849 T cells are central effector cells of the immune system. CD8+ T cells recognize and kill antigen presenting cells expressing foreign peptides. CD4+ T cells facilitate both cell-mediated and humoral immune responses by providing help to activation of cytotoxic killing (macrophages, natural killer cells, and CD8+ T cells) and to the generation of antibody responses by B cells. Overactivation of T cells leads to self destruction as seen in autoimmune disorders and in graft-versus-host disease (GVHD). The immune system has several mechanisms to control ...

Compositions and Method for Detection of Amines and Amino Acids

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:30 GMT

TechID: J1530 Researchers at the University of Pennsylvania have synthesized a novel fluorogenic reagent for detection of amino functionalities. Uses of the reagent include detection and assay of peptides, amines, amino sugars, and amino acids in solutions and on surfaces. At present a wide range of fluorogenic or chromogenic compounds are employed by forensic scientists for use in latent fingerprint development and identification. One significant drawback of presently existing analogs is that they are prohibitively expensive for most crime laboratories: about $40/gram. The new reagent is...

Constrained and Modified Ligands

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:25 GMT

TechID: G1141 Various synthetic methods of peptide formation are widely available. However, the problem with these synthetic peptides is that their conformation and activity fluctuate greatly depending upon their environment and method of synthesis. These flutctuations may preclude the practical application of the peptides to bind and mediate the required biological effect. Recently researchers at the University of Pennsylvania have developed a novel method for the synthesis of constrained peptides which have free aromatic amino acid residues linked to them. These constrained pepti...

Use of Monoclonal Antibodies Against Human HveA in the Detection of Cellular HveA Expression, as Modulators of the Immune System, as Anti-Herpesviral Reagents and in HveA Purification

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:23 GMT

TechID: M2161 Herpes simplex virus enters cells by a multli-step process involving several viral envelope glycoproteins and one of at least three recently identified proteins expressed on the surface of the host cell. One of the cellular proteins which mediates HSV entry, HveA, is a newly described member of the tumor necrosis factor receptor (TNFR) family of proteins. HveA is abundantly expressed in lymphoid tissues. Subsequent to its identification as a mediator of HSV entry, HveA was shown to specifically bind HSV gD (explaining its role in HSV entry) as well as human...

Inhibitors of Arginine Metabolizing Enzymes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:22 GMT

TechID: K1661 Researchers at the University of Pennsylvania have found that the synthesis and composition of compounds with the following structure have been found to be effective inhibitors of arginase: HOOC-CH(NH2)-Y1-Y2-Y3-Y4-B(OH)2, where Y1, Y2, Y3, and Y4 are selected from the group consisting of CH2, S, O NH, and N-alkyl. Two compounds in particular, called ABH and BEC have been shown to be especially potent inhibitors of arginase. Binding assay data shows that these compounds are the most potent inhibitors of arginase reported to date. These compounds ar...

Contrin---A Target for Fertility Regulation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:16 GMT

TechID: K1737 In response to the rapidly increasing rate of world population growth, excellent advances have been made in male contraception research. These discoveries have focused primarily on vas occlusion via percutaneous injection with sclerosing agents, polyurethane elastomer plugs, or silicone plugs, and on suppression of spermatogenesis by hormonal methods such as androgens and GnRH agonists. However, failure to achieve azoospermia in some men, the high cost of the reagents, and the prolonged lag time before the effects are seen indicate the need for additional m...

Anti-Angiogenic Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:12 GMT

TechID: F1019 It has been shown that the combination of certain “angiostatic” steroids, which by themselves have little or no action toward inhibiting neo-vascularisation, become anti-angiogenic when combined with certain preparations of heparin and sulfated cyclodextrins. This technology has been used to produce various formulations of physically mixed components to be used as inhibitors of angiogenesis. Researchers at the University of Pennsylvania have produced a novel group of individual chemical compounds in which one part of the chemical structure is a...

HUP Light; Resorptive Pit Assay

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:09 GMT

TechID: K1869 Paget's disease is a highly degenerative human bone affliction which affects approximately 3% of the population over 45 years of age. The disease is characterized by increased bone cell turnover, involving osteoclasts and osteoblasts, and is due to both cellular and environmental factors. Osteoporosis is also a bone thinning disease that leaves sufferers vulnerable to hip and spine fractures, deformities and chronic pain. A 1997 report estimates that the global osteoporosis therapeutic market will expand from $3.1 Billion to $9.5 Billion in 2003. Such figu...

Deformable Models with Parameter Functions for Heart Shape and Motion Analysis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:05 GMT

TechID: H1299 Conventional cardiac imaging methods can reliably display the information on the three dimensional structure of the walls of the heart chambers if examined by an expert. However, to calculate the volume of these chambers or to quantify the heart motion, and hence to assess cardiac function, a computer-based approach must be able to identify this three-dimensional structure imbedded in the image. Because of the image noise, computer programs are unable to unambiguously identify and quantify this three dimensional structure embedded in the image and manual intervention i...

Sulfonamide-Based Compositions and Methods

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:05 GMT

TechID: H1263 Many bacterial strains have become increasingly resistant to known antibacterial compounds. There remains a need for novel antibacterial agents to which commonly-encountered bacteria are not resistant. University of Pennsylvania researchers have designed novel antibacterial agents which are members of a new class of sulfonamide-containing compounds. Specifically, researchers have developed methods for synthesizing this novel class of compounds, providing chemical intermediaries employed in the synthesis as well as antibacterial compositions comprising the compounds.&...

Subcutaneous Islet Transplantation and Retroperitoneal Islet Transplantation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:23:01 GMT

TechID: T4445 Type 1 Diabetes Mellitus (T1D) is a chronic autoimmune disease in which the immune system destroys the insulin producing beta cells in the pancreas. In the absence of insulin, the body cannot absorb glucose. Therefore, patients with T1D need daily insulin administration for survival and to monitor their glucose levels. Hyperglycemia (high glucose levels) damages organs (blindness, kidney failure, lower limb amputation, heart disease, and stroke), and hypoglycemia (low glucose levels) may lead to hypoglycemic coma and even death. A definitive treatment of T1D would be to r...

A New Class of Siloxane Polymers for the Low Temperature Desposition of Sicion Dioxide Films

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:59 GMT

TechID: H1274 University of Pennsylvania researchers have collaborated in the development of a new class of silane (SiO2) polymers capable of being processed into SiO2 thin films. The resulting SiO2 films, formed by the hydrolysis and condensation of silane, are highly uniform and defect free. Defect free coatings cannot be easily achieved using the currently available methods such as spin-on glass (SOG) or chemical vapor deposition (CVD). The thickness of these films can be varied from about 200 to 5000 angstroms. Multiple coatings can be used to prep...

A Novel Microarray Technique for Nucleic Acid Expression Analysis

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:58 GMT

TechID: Q3345 RNA expression profiling has become an invaluable tool in assessing transcriptional levels in various physiological states. cDNA microarrays are the most common high-throughput method used for RNA expression profiling, but they have some limitations. DNA microarrays require extensive pre-hybridization manipulation of the sample, and any of theses steps can introduce bias in the results. Current microarray technologies can also only be used to amplify mRNA species, and are not applicable to expression profiling of many of the small regulatory RNAs. Re...

Rabbit Bladder Smooth Muscle Cell Line

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:50 GMT

TechID: S4040 Researchers at the University of Pennsylvania have established a cell line from hypertrophied rabbit urinary bladder smooth muscle (SM) that stably expresses SM myosin (SMM). These cells, termed BSM, are spindle shaped and form swirls, similar to the "hills and valleys" described for cultured aortic SM cells. Western blotting revealed that BSM expresses the amino-terminal SMM heavy chain isoform SM-B, the carboxy-terminal SM1 and SM2 isoforms, and SM alpha-actin. In addition, they express cGMP-dependent protein kinase G, made by contractile SM c...

Rapid Analysis of Gases for MIGET Using Membrane Countercurrent Exchange and Membrane Inlet Mass Spectrometry

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:48 GMT

TechID: J1499 The Multiple Inert Gas Exchange Technique (MIGET) is an accepted method for accurate measurements of pulmonary gas exchange. MIGET is already used for diagnosis, prognosis, and treatment of lung disease in research laboratories around the world. The technique measures a patient’s pulmonary ventilation/perfusion ratio to analyze lung disease. However, current MIGET methods rely on gas chromatography to determine the concentrations of inert gases required for the measurement. Unfortunately, gas chromatography requires a skilled technician and two...

Nitration of Tau Protein is Linked to Neurodegeneration in Tauopathies

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:41 GMT

TechID: Q3448 Oxidative and nitrative injury is implicated in the pathogenesis of Alzheimer's disease (AD) and Down Syndrome (DS). There is no direct evidence that links this type of injury to the formation of neurofibrillary tau lesions. The researchers at Penn have generated a monoclonal antibody (n847) which recognizes nitrated tau and a-synnuclein. (American Journal of Pathology, 163, 1021-1031, 2003). This antibody is distinct from the pan anti-nitrated tyrosine-residue specific polyclonal antibody (anti-3-NT) which recognizes 3-NT residues regardless of the protein context. App...

Urokinase Kringle-Mediated Cell Signalling

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:37 GMT

TechID: M2231 Urokinase is a natural enzyme that the body uses to dissolve clots and to facilitate cell migration. Accordingly, this enzyme should have therapeutic utility for diseases caused by clotting. The urokinase molecule consists of several domains, one of which is the kringle domain. A University of Pennsylvania research team has examined the effect of urokinase kringle on endothelial cell and smooth muscle cell function. The researchers studied the effect of this kringle subunit on the contraction of rat aortic rings and demonstrated that urokinase kringl...

Targeting of Drugs to the Pulmonary Vascular Endothelium by Antibody Against PECAM-1

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:37 GMT

TechID: K1670 Pulmonary endothelium is an important target for therapeutic intervention, especially for the protection against oxygen toxicity, the stimulation of pulmonary fibrinolysis, and as a site for gene therapy. The current methods to target "effectors" to the pulmonary endothelium, including use of the anti-angiotensin converting enzyme antibody, are inefficient for the intracellular targeting of large amounts of effector. This invention describes a modified antibody that efficiently targets the pulmonary endothelium in mice and rats upon injection into the anim...

Non-Steroid and Non-Prostanoid Inhibitors of Steroid and Prostaglandin Transforming Enzymes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:34 GMT

TechID: D572-C-01 Diseases of the prostate are prevalent among adult men. Fifty-percent of men above the age of 65 will experience benign prostatic hyperplasia (BPH). The prostatic carcinoma (PCA) is the second leading cause of cancer death in men. Both diseases are androgen dependent and are treated with androgen ablative therapy. This technology developed by researchers at the University of Pennsylvania is comprised of small molecule non-steroidal inhibitors that will prevent the formation of active androgen and therefore will decrease androgen level...

Salivary Glycoproteins Inhibit HIV Infectivity

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:32 GMT

TechID: K1773 Although HIV nucleotide sequences have been identified and isolated of from oral samples such as saliva, the isolation of infectious HIV virus from the oral cavity of a patient is rare, and when present, viral titers are low. Several reports have demonstrated the presence of HIV nucleotide sequences using PCR in up to 80% of oral samples obtained from seropositive individuals; however, infectious virus could only be isolated from less than 5% of these individuals. The striking discrepancy between the physical evidence for virus in saliva and the lack of in...

Uniparental Cells as a Source of Stem Cells for Therapeutic Applications

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:29 GMT

TechID: Q3602 Human embryonic stem (ES) cells are hailed for their promising therapeutic potential in a variety of human diseases. Since these stem cells are derived, however, from potentially viable human embryos, their production and use has serious ethical and legal complications. Researchers at the University of Pennsylvania have demonstrated for the first time that transplantation of mammalian uniparental cells into lethally irradiated adults resulted in long-term, multi-lineage reconstitution of the hematopoietic system. In the mouse, embryonic-stem cell derive...

Biomimetic Iron-Oxide-Containing Lipoprotein and Related Materials

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:22 GMT

TechID: S4149 Engineered lipoproteins for magnetic imaging and drug delivery Targeted delivery by biocompatible vesicles is an active area of drug development, owing to its potential to reduce toxicity and enhance efficacy. One of the most attractive platforms is lipoproteins which are more stable than liposomes. In its natural form, lipoproteins include high density lipoprotein (HDL) and low density lipoprotein (LDL) that transport water-insoluble lipids such as cholesterol in the blood. Natural lipoproteins, however, are delicate and cannot be easily coerced to alter its paylo...

Reduced Power Selective Excitation in Magnetic Resonance and Optics

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:21 GMT

TechID: G1163 In Magnetic Resonance Imaging (MRI) and optical spectroscopy there is a need to design frequency profiled rf pulses which selectively excite only a part of the spectrum. In designing such pulses the peak power is limited by the transmitter power and the total energy is limited by concerns over sample (or tissue) heating. University of Pennsylvania researchers have invented an algorithm for the synthesis of reduced power pulses which contain the required frequency profile. The algorithm will yield pulses that require either less peak amplifying ...

Murine Monoclonal Antibody to gIIIp Protein of VCSM 13 Helper Phage

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:19 GMT

TechID: M2205 Vectors based on the filamentous phage VCS M13 have been developed to display antibody fragments and peptides fused to the various phage coat proteins. The most commonly used of these is the ligand-coat protein three (gIIIp), which upon expression is displayed on the surface of the phage particle. Selection of displayed antibody/peptide-ligand with its receptor is studied by incubating the phage with an immobilized antigen, and the bound phage with relevant specificity can then be further purified by elution, and ...

Wavelets and the Fractal Structure of the ECG

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:18 GMT

TechID: G1133 The detection of ventricular tachycardia, a possibly fatal cardiac arrhythmia, is a critical medical issue. However, using current technologies, doctors can not accurately identify patients at risk for this deadly disease. In response to this critical problem, University of Pennsylvania researchers have developed a novel solution: wavelet analysis, a mathematical tool that decomposes non-linear signals. Researchers have been able to detect ventricular tachycardia using wavelet analysis on ECG signals, electrical activity measurement of the heart, a...

Novel Use of the Irritating Principle, Oleotussin, in Olive Oil as well as Structurally and Functionally Similar Compounds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:15 GMT

TechID: R3881 A desirable attribute of many premium olive oils is the distinctive irritation or pungency that is perceived at the back of the throat. This sensation is similar to that caused by ibuprofen and other non-steroidal anti-inflammatory drugs. Scientists at the Monell Center and University of Pennsylvania have developed an effective enantioselective synthesis of "oleocanthal", which is largely responsible for the irritation (pungency) of olive oil. The sensory effects of entantiomers of oleocanthal are similar in quality and anatomical location to...

High Weight-fraction Surfactant Solubilization of Single-wall Carbon Nanotubes in Water

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:14 GMT

TechID: P2952 Background Successful development and manufacturing of new materials, and advancement of technologies relying on use of single-wall carbon nanotubes (SNWT) greatly depend on availability of the key component, SNWT, at low cost and in a form convenient for further processing. Current purification technologies are expensive and have very low yield, which increases the cost of already expensive raw, contaminated SNWT, by more than one order of magnitude, from about $60/g to $750/g. Drs. Arjun Yodh and Mohammad Islam at the University of Pennsylvania have developed ...

Combined Therapy with Chemotherapeutic Agents &Herpes Simplex Type 1 (HSV-1) ICP 34.5 Mutants for Treatment of Cancer

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:11 GMT

TechID: K1787 Lung cancer is the leading cause of cancer death in the United States. Despite earnest efforts, prognosis for lung cancer is still very poor and most patients die within one year of diagnosis. Cancer therapy has included chemotherapy, radiation, surgery and most recently gene therapy. The combined use of chemotherapy, radiation, and surgery has augmented the benefits of these therapies in some types of cancer but in only a few types of cancer has it resulted in eradication of the tumor. Researcher at the University of Pennsylvania have demonstrated that th...

Angiography and Quantitative Blood Flow Measurement

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:10 GMT

TechID: F973 The measurement of flow of fluid in tissue (tissue perfusion), and of blood flow in arteries is important for the functional assessment of organs in vivo. Researchers at the University of Pennsylvania have invented a new subtractive technique for MR angiography in which proton spins in the arterial supply are inverted in a steady state fashion by applying a constant amplitude off-resonance RF pulse in the presence of a constant magnetic field gradient aligned along the arterial direction. Spins moving in the direction of the gradient are inverted and an angiogram...

Ectopic Expression of Urinary Plasminogen Activator (uPA) in Platelet as a Novel Anti-Thrombotic Strategy

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:08 GMT

TechID: O2906 Thrombosis caused by platelet aggregation plays a central role in major diseases such as myocardial infarction (MI). MI patients treated by coronary angioplasty have a high risk of thrombotic re-occlusion. Therefore, a therapy that can prevent thrombosis of the repaired blood vessels is highly desirable. University of Pennsylvania researchers have developed a novel anti-thrombotic gene therapy strategy. They proposed that a certain thrombolytic gene could be specifically expressed in platelets to prevent pathological thrombosis. Penn researche...

A Surgically Implantable Antipsychotic Drug Delivery System

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:06 GMT

TechID: M2281 Penn researchers have developed a novel and simple method to continuously deliver anti-psychotic drugs, to the approximately two percent of the human population suffering from schizophrenia. Current means of administration of anti-psychotic drugs often lead to loss of patient compliance. Poor compliance is the leading cause for relapse and re-hospitalization among schizophrenia patients. It is estimated that from 50% to 80% of people with schizophrenia do not adhere to medication as prescribed, and that over 80% of non-compliant patients will relapse withi...

Subvoxel Processing: Method for Reducing Partial Volume Blurring in Tomographic Images

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:22:05 GMT

TechID: M2268 Currently, partial volume blurring is a problem when the image voxel size is larger than the imaged structural element, a particular issue for in vivo imaging. In an effort to develop a better method of dimensional measurement in such situations, University of Pennsylvania researchers have developed a subvoxel process, which alleviates the constraints ordinarily imposed on voxel size in digital images. Subvoxel processing is based on two assumptions: (1) smaller voxels are more likely to have high bone volume fraction (BVF) and (2) bone is generally in close pr...

Method for Non-Invasive Diagnosis of Osteoporosis by Magnetic Resonance

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:59 GMT

TechID: D735 Osteoporosis is a widespread disease predominantly afflicting postmenopausal women. The two currently used methods for diagnosis and therapy (SPA/DPA and QCT) are both invasive, involving ionizing radiation, and are limited in that they only measure bone mineral density (BMD), while ignoring the morphology of the trabecular bone structure. Moreover, these methods fail to take into account the physiological and /or biochemical state of the marrow. In order to improve present diagnostic methods, researchers at the University of Pennsylvania have developed a revolutionary...

Hair Derived Melanocyte and Melanoblast Transplant to Treat Vitiligo

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:55 GMT

TechID: S4212 Vitiligo is a skin condition of white patches resulting from loss of pigment due to loss of melanocytes in the skin. White or depigmented patches of skin can appear on different parts of the body; and any part of the body may be affected. Approximately, 1 to 2 percent of the world population has vitiligo. Vitiligo is more common or appears more pronounced on darker or non-European skin. Roughly fifty percent of sufferers develop vitiligo before twenty five years of age. Current medically acceptable treatment options include steroid creams with or wit...

TSG-1, a Novel Marker and Potential Determinant of Anti-Diabetes Drug Action

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:54 GMT

TechID: L2066 Resistin, is a novel adipocyte-secreted hormone originally discovered in the laboratory of Mitch Lazar at the University of Pennsylvania. In mice, circulating levels of Resistin are increased in both high-fat diet-induced and genetic models of obesity and diabetes. Neutralizing antibodies directed against Resistin raise blood sugar levels when administered in vivo and recombinant Resistin impairs glucose tolerance, confirming Resistin's role in insulin resistance. In humans, genetic evidence suggests that Resistin not only plays a role in obesity, insulin resistance...

Targeting of Drugs to the Luminal Surface of the Pulmonary Endothelial Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:50 GMT

TechID: K1850 Fibrinolytic therapy with plasminogen activators (PA) is limited by its extremely rapid elimination from the bloodstream due to the uptake by a non-target tissues. Researchers at the University of Pennsylvania have developed a novel technology that allows the immunotargeting of drugs, such as plasminogen activators and anticoagulants, to the luminal surface of the pulmonary vascular endothelium. The technology involves the conjugation of active drug substances to antibodies to cellular adhesion molecules (specifically anti-ICAM-1) which allows for tissue specifi...

Function and Regulation of Angiopoietin 4 (Combined into P3172)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:48 GMT

TechID: P3222 Angiopoietins (Angs) have a crucial regulatory role in angiogenesis. Angiogenesis plays an important role in embryogenesis and tumorigenesis. Four Angs have been identified to date. The role of Ang-1 and Ang-2 as regulators of angiogenesis during both vascular maintenance/development and pathologic states such as cancer has been documented. However, very little is known about Ang-4 and its mouse homolog Ang-3. Researchers at the University of Pennsylvania have discovered several novel functions of Ang-4 which define its role as an important target i...

Regulation of Fasted Blood Glucose by Resistin

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:46 GMT

TechID: Q3428 The association between obesity and diabetes supports an endocrine role for the adipocyte in maintaining glucose homeostatis. Resistin is an adipocyte-secreted hormone that has been implicated in diabetes (EMBO journal, 19, 4046, 2000; Journal of Biological Chemistry, 276, 11251, 2001). Howeever, the role of resistin in glucose metabolism is controversial (Journal of Clinical Investigations, 111, 173, 2003)and the normal physiological function of resistin is unknown. The investigators at Penn have generated mice lacking the adipocyte hormone resistin (Science, 303, 1195-1198...

Technique for Identifying Fuzzy Objects in Multi-Dimensional Images

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:44 GMT

TechID: H1273 University of Pennsylvania researchers have developed an imaging resolution enhancement technology that solves a major problem in any application in which objects within images need to be more clearly defined. All objects are fuzzy to some extent, due to the limits of resolution of any imaging technique. This limitation is a problem in deciphering images in a medical scan or an identifying photograph. The University’s technology can be used to quantify fuzzy images from magnetic resonance imaging and other procedures. It is especially use...

Murine Ovarian Cancer Cell Line Overexpressing VEGF

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:38 GMT

TechID: O2701 Ovarian cancer is the most frequent cause of death from gynecologic malignancy, with the 5-year survival rate at <30%. Since early stage ovarian cancer tends to go unnoticed, little is known about the origins and early progression of the disease. It has been shown that the majority of epithelial ovarian cancers express high levels of vascular endothelial growth factor (VEGF), which has been implicated in tumor angiogenesis, disease progression, and ascites formation. University of Pennsylvania faculty have...

Sequential crosslinking to alter network properties

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:36 GMT

TechID: V4931 Please call licensing director for more information Applications ? Tissue regeneration-differences in cellular morphology in patterned AHA hydrogels could be useful as a signaling mechanism for spatially controlled differentiation of encapsulated stem cells. ? Regeneration of tissues with anisotropic properties (e.g., vasculature or nervous tissues) or where spatially controlled organization of cells is desired Advantages ? Multimode cross linking on the same cell encapsulated hydrogel ? Use of photomasks during sequential cross linking offer improved spatial c...

Therapy-Resistant Hypoxia; Detection by Nitroheterocyclic Metabolism

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:28 GMT

TechID: D842 Hypoxic cells are the most familiar form of treatment-resistant cells found in certain tumors, vascular diseases or after a stroke. These cells have an inadequate supply of blood and thus have very low concentrations of oxygen, which allow resistance to killing by radiation and chemotherapy. Under the influence of low oxygen pressure, nitroheterocyclic drugs undergo a bioreductive metabolism which forms adducts with cellular proteins from hypoxic cells. University of Pennsylvania researchers have developed novel halogenated 2-nitroimidazole derivative...

Rat anti-TRANCE-R monoclonal Ab

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:25 GMT

TechID: P2932 Lymph nodes (LNs) are critical secondary lymphoid organs for the initiation of immune responses. The proper LN development requires the functions of TRANCE (tumor necrosis factor-related activation induced cytokine) and TRANCE receptor (TRANCE-R) pathway. Inactivation of murine TRANCE or TRANCE-R results in the absence of all peripheral and mesenteric lymph nodes. University of Pennsylvania researchers have generated a rat anti-TRANCE-R monoclonal antibody. This antibody has been successfully used to detect cell surface TRANCE-R in FACS analysis (as referenced i...

PCIF1 as a Target for the Improvement of Pancreatic Islet B Cell Mass and Function in Diabetes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:20 GMT

TechID: T4450 Pancreatic-duodenal homeobox 1 (PDX-1) is a Hox-type protein that is critical for normal pancreas development and the proper differentiation of the endocrine pancreas. In humans, PDX-1 gene mutations in the encoded C-terminal domain, cause pancreatic agenesis and early- and late-onset Type 2 Diabetes. Dorris Stoffers, MD, PhD, has discovered a novel nuclear PDX-1 C-terminal interacting protein (PCIF1) is expressed in insulin-producing pancreatic beta cells and in a subset of exocrine acinar and duct cells. PCIF1 inhibits PDX-1 transactivation of target gene promote...

HiDAC: High Density Autonomous Crowds: Modeling and Simulation

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:11 GMT

TechID: T4458 Simulating the motion of realistic, large, dense crowds of people is still a challenge for the computer graphics community. Each person is represented as an autonomous agent who can navigate, react and make decisions individually. Typical computer graphics approaches either resemble particle simulations (where agents lack orientation controls) or are conservative in the range of human motion possible (agents lack psychological state and are not allowed to 'push' each other). Researchers at the University of Pennsylvania have developed software that simulates th...

SPAMM IV System and Method for Magnetic Resonance

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:08 GMT

TechID: E881 Magnetic resonance imaging (MRI) of moving body parts, such as the beating heart, is difficult since the time constants for acquiring MRI data are long compared to the speed of motion of these body parts. University of Pennsylvania researchers have developed a technique known as Spatial Modulation of Magnetization (SPAMMÒ), which ‘marks’ heart walls so that useful MRI images can be obtained. The SPAMMÒ images are produced by first magnetically ‘marking’ the heart and then scanning the same section. By noting the displacement of the ‘marked...

Angiopoietin and Fragments, Mutants, and Analogs thereof and Uses of the Same

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:05 GMT

TechID: P3115 Angiopoietins play an important role in maintaining vascular integrity. Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are crucial regulators of angiogenesis during both normal development and tumor growth. Researchers at the University of Pennsylvania have developed Ang-1 mutants and therapeutic methods using such compounds for treatment of diseases related to vascular malfunction and cancer. The novel Ang-1 mutants are more potent and stable than wild-type Ang-1. Potential applications include prevention and treatment of vascular d...

Characterization and Use of a Human Prolactin Binding Protein (hPRLBP)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:05 GMT

TechID: N2491 The actions of prolactin are mediated by its receptor, a member of the superfamily of single transmembrane cytokine receptors. High affinity binding proteins for the closely related growth hormone have been found in the sera of several species including humans. In contrast, this disclosure is the first time that conclusive evidence has been presented that an analogous prolactin binding protein is expressed in human serum. Data are available that show that the prolactin binding protein antagonizes prolactin action. Prolactin binding protein is also cap...

The Generation and Use of Recombinant Forms of the Vaccinia Virus L1R and A33R Proteins as a Subunit Vaccine

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:02 GMT

TechID: P3036 Please call licensing director for more information Applications Recombinant envelope proteins could be used as a subunit vaccine for immunization of human subjects against vaccinia and possibly variola viruses. The recombinant proteins could also be used to mitigate the adverse complications of the attenuated vaccinia vaccine High affinity human antibodies generated from the recombinant proteins could be used as effective post-exposure treatment against vaccinia or variola viruses. Advantages High yields of purified recombinant proteins can easily be collected. R...

Identification of a Novel P2 Purinoceptor as New Anti-Ischemic and Insulin-Mimetic Target

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:21:02 GMT

TechID: K1788 The association of diabetes with atherosclerosis has been well documented. In addition, there are indications that patients with diabetes often develop a cardiomyopathy which can lead to contractile failure independent of coronary artery disease. Researchers at the University of Pennsylvania have identified a new cardiac purinoreceptor that mediates a pronounced stimulation of basal and insulin-dependent glucose transport into the cardiac myocyte. Since enhanced glucose uptake into the cells is an important therapeutic modality in treating patients with insulin resistanc...

Novel Oxazolidinones

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:55 GMT

TechID: J1580 Since peptides play an important role in many biochemical processes, there is a great need in research and therapeutics for peptide mimics that are resistant to degradation by proteolytic enzymes. With evidence that hydrogen bonding is necessary for bonding between peptidyl inhibitors and proteolytic enzymes, non-peptidal enzyme inhibitors must at least in part mimic such hydrogen bonding capabilities in addition their ß-strand conformations. Usi...

3 Dimensional Multislice T1rho (Spin-Lock) Imaging

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:51 GMT

TechID: N2436 Current existing T1r (spin-lock) methods are only able to acquiring single slice images. Additionally, this method is not able to handle 3D or multi-slice imaging and is highly sensitive to radio-frequency homogeneity. University of Pennsylvania researchers have developed a more stable Spin-locking Magnetic Resonance Technology that is capable of measuring physiological information. The technology is based on T1r (spin-lattice relaxation in the rotating frame) and is primarily focused on non-invasive MR methods to detect and monitor physiological changes. The te...

B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J - TRM

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:47 GMT

TechID: T4424 Filamentous tau inclusions are hallmarks of Alzheimer’s disease (AD) and related tauopathies, but earlier pathologies may be responsible for the onset of disease. To investigate this, the inventors have studied P301S mutant human tau transgenic (Tg) mice. Filamentous tau lesions develop in the P301S Tg mice by 4-6 months of age, and progressively accumulate in association with striking neuron loss as well as hippocampal and entorhinal cortical atrophy by 9-12 months of age. Importantly, hippocampal synapse loss and impaired synaptic function are dete...

Chimeras of Nicotinic Acetylcholine Receptor Subunits

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:45 GMT

TechID: Q3379 Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels expressed mainly in the nervous system and at the neuromuscular junction, although they also are expressed elsewhere. They are members of the superfamily of nicotinoid receptors, which includes -aminobutyric acid (GABA) type A and C, glycine, and serotonin 5-HT3 receptors. They contain five homologous subunits, with subunits designated alpha 1-10, beta 1-4, etc . Each subunit is an integral membrane protein with a large, ligand-binding N-terminal extracellular domain, four transmembrane domains (M1-M4),...

Heterologous Protein Production

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:42 GMT

TechID: P2956 Protein-based therapies constitute a growing portion of pharmaceutical and biotechnology pipelines. The cellular process of protein secretion has been heavily exploited for heterologous protein production on an industrial scale. However, recombinant proteins are currently engineered to be secreted via the Sec pathway. This pathway transports proteins in an unfolded state and is thus useless for exporting a significant number of proteins that fold prematurely or are unable to fold correctly in the extracytoplasmic environment. Researchers at the Univ...

Inhibitors of Human Cathepsin L to block SARS, EBOLA, and Hendra virus (Combined into U4783)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:40 GMT

TechID: U4734 Cathepsin L is a lysosomal cysteine protease involved in several biological processes some of which include epidermal hemeostatis, hair follicle morphogenesis and cycling, extracellular matrix degradation and pathogen infectivity, and viral entry into cells. Investigators at Penn have invented two novel molecular structures and synthetic pathways for compounds that inhibit human cathepsin L. They have conducted in-vitro mammalian cell assays that have shown these compounds to be highly potent against Cathepsin L. Furthermore, these assays have demonstrated the foll...

Oxidative Damage Linked to Neurodegeneration by Selective alpha-Synuclein Nitration in Synucleinopathy Lesions

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:36 GMT

TechID: N2447 Oxidative injury has been implicated in the pathogenesis of neurodegenerative disorders including Alzheimer's disease (AD), Parkinson's disease (PD), Dementia with Lewy bodies (DLB), Amyotrophic lateral sclerosis and Huntington's disease. Oxidative damage occurs when reactive oxygen species is overproduced which can damage cellular components including nucleic acids and proteins. Both reactive oxygen and nitrogen species act synergistically to modify protein tyrosine residues to 3-nitrotyrosine (3-NT). Alpha-synuclein (alpha-syn) is a highly conserved protein that is abundan...

Immunoassay for Isoprostanes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:35 GMT

TechID: K1847 There has been considerable confusion as to how one might identify human diseases in which increased oxidant stress plays a role. Similarly, there has been no biochemically rational basis for the selection of drugs or vitamins as potential antioxidant therapies. This is of particular importance as many common antioxidant vitamins are actually pro-oxidant at certain levels in vitro. This has also been speculated to underlie the adverse effects and conflicting results of antioxidant therapy in animal models of disease and in clinical syndromes thought to be a...

Self-Assembled Bioresorbable Polymersomes

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:29 GMT

TechID: S4031 See E960 parent docket. Applications Advantages

Fast Photoconductivity in Self-Assembled Columnar Liquid Crystalline Photorefractive Materials

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:28 GMT

TechID: O2806 There is a growing interest in and need for optoelectronic materials that exhibit high charge carrier mobility while remaining easy to make and process. Currently available electrically conductive organic crystals have high mobilities, but are usually difficult to produce whereas conducting polymers are known for their ease of processing and low mobility. Researchers at the University of Pennsylvania have addressed these limitations by creating self-assembling, self-organizing organic nanostructures with controlled optoelectronic properties. Functionaliz...

Method for Preparation of Electron Deficient Porpyrins

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:26 GMT

TechID: G1116 Current synthetic methods for producing porphyrins produce low yields and do not exist for many types of porphyrins. University of Pennsylvania researchers have developed an improved method for synthesizing a wide variety of electron deficient and strapped porphyrins. Applications Advantages

Fibrous Composite for Tissue Engineering

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:25 GMT

TechID: O2779 Bone repair and tissue engineering are multi-billion dollar markets growing 7 to 9% per year. Materials used in bone transplants and scaffolds for tissue restoration must have a particular combination of features to serve their purpose well. Since none of currently available materials is perfect for these applications, several of the best candidates are being used, each with its shortcomings. For example, dried silica gels are very attractive because of their nanometer-sized pores, but they lack larger pores (~100 mkm) that exist in natural bone, and are fragile. To o...

Novel Approaches to Develop HSV-1 and HSV-2 Vaccines

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:22 GMT

TechID: S4232 This is a live attenuated virus designed to prevent spread of the virus to axon fibers and hence the neuronal nucleus, which is the site of HSV latency. Human infection with herpes simplex virus (HSV) types 1 and 2 is typically acquired through intimate contact and causes oral and genital lesions. HSV-1 usually causes oral ulcers and HSV-2 normally causes genital ulcers, but the reverse can also occur. Once a person is infected with HSV-1 or HSV-2, they will always be carriers of the virus. After the initial infection, lesions heal and HSV exists in a dormant, latent...

A Nanometer-Sized Carrier for Diagnostic Imaging, Gene Therapy and Drug Delivery

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:19 GMT

TechID: O2835 Synthesis, functionalization and surface treatment of nanoparticles is an arfea of utmost importance in the field of medical nanotechnology. Controlling the surface chemical composition and mastering its modification at the nm-scale are the central issues for high-added value applications involving nanoparticles. Surface-modified silica nanoparticulates and nanofibers have received much interest due to its monodispersity and high degree of shape uniformity. Spherical nanoparticulates are of special interest since they are ideal for acting as carriers to specific body s...

Structure-based Design of LpxC Inhibitors (Combined into P3038)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:20:09 GMT

TechID: P2980 Sepsis and septic shock are diagnosed in 600,000 individuals a year in the US and these conditions result in 100,000 deaths annually. Many of these cases are triggered by Gram-negative bacteria that cause the overexpression of cytokines and other inflammatory mediators in response to macrophage activation. The lipopolysaccharide (LPS) barrier found in the sheath of Gram-negative bacteria is a major obstacle to the development of safe and effective antibiotics to treat such infections. Researchers at the University of Pennsylvania have determined t...

Utrophin upregulation via inhibition of microRNA's

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:59 GMT

TechID: U4833 Background and Rationale Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disease, pathogenically stemming from a lack of functional dystrophin protein. DMD is one of nine major muscular dystrophies and progressively affects voluntary muscle movement. Characteristics of DMD include degenerative muscle wasting and weakness, culminating in lethality by age 30. While the etiology and pathogenesis of the disease are well defined, DMD remains a devastating and incurable affliction. The technology herein details novel methods to upregulate utrophin protein ...

A Method to Inhibit Complement Activation by A Novel peptide Against a Functional Domain of Human Complement C3

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:52 GMT

TechID: I1374 Inflammation occurs as part of the immune response to a stimulatory antigen encountered by the immune system. This response can be either beneficial, as is the case in the recognition of bacteria and other pathogens in the blood stream, or detrimental to the health of an individual. Examples of harmful inflammatory responses include tissue damage resultant from several autoimmune diseases such as type II collagen induced arthritis, myasthenia gravis, lupus erythematosus, hemolytic anemia, glomerulonephritis, and immune complex induced vasculitis. In these conditions, ...

A Neprilysin Polymorphism as a Screening Tool for the Development of Amyloid-Beta Plaques Following Brain Trauma

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:51 GMT

TechID: U4671 Amyloid ? deposition in brain is an important milestone in the development of Alzheimer’s disease. Neprilysin plays an important role in A? metabolism. Reduction in Neprilysin protein and Neprilysin mRNA level correlates well with an increase Amyloid ???plaque density. Several researchers have tried to explore the relationship between NEP gene DNA sequence and Alzheimer’s Disease. However, the studies on Japanese and Swedish population samples have not been able to link Alzheimer’s Disease with polymorphisms in NEP gene. Traumatic brain injury ...

Zeta Mouse

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:42 GMT

TechID: K1663 The zeta-globin gene is normally expressed only during certain stages of gestation in humans and mice. Expression is limited to the red blood cells. For this reason, the zeta-globin gene product is an excellent marker for use in the separation of fetal cells isolated from the mother’s blood. The invention described here is a transgenic mouse, referred to as the “Zeta Mouse” which expresses the human zeta-globin gene throughout development and into the adult stage. Applications Advantages

Modulation of Receptor and Receptor Subtype Affinities Using Enantiomeric and Diastereomeric Monosaccharides as Peptidomimetic Scaffolds

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:39 GMT

TechID: J1640 Peptides are implicated in a wide variety of biochemical processes in humans and other mammals. Many hormones and neurotransmitters are controlled by receptor-mediated stimulation of guanine nucleotide-binding regulatory proteins, also known as G-proteins. One of the predominant players in cellular signal transduction systems, G-proteins activate or inhibit different effector enzymes, which modulate the levels of intracellular second messengers involved in biochemical pathways. Peptides have had ...

Oil-Flair Optimized Inter-Leaved Fluid Attenuation Inversion Recovery

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:30 GMT

TechID: H1205 Cerebrospinal fluid (CSF) produces a strong signal that appears as overly bright areas in a magnetic resonance image. The signal from CSF can obscure subtle contrasts in the image produced from lesions or disease processes. Further artifacts in the image are also introduced by the flow of CSF during scanning. Fluid Attenuation by Inversion Recovery (FLAIR) can be used to suppress the CSF signal. In this technique, an inversion pulse precedes each multi-slice spin-echo sequence. Although this approach sufficiently reduces the signal from CSF, th...

Human Genes with Anti-HIV Function

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:28 GMT

TechID: O2865 Human immunodeficiency virus type-1 (HIV-1) is the etiologic agent for AIDS. Current medical treatments for HIV infections include combinations of drugs that inhibit the action of the essential virus encoded enzymes reverse transcriptase and protease, however significant problems of drug-failure persist. Thus, in the absence of effective AIDS vaccines, the range of anti-HIV drugs needs to be expanded. As with all viruses, HIV-1 growth and replication requires the concerted action of viral as well as cellular factors. University of Penn...

Ex Vivo Remodeling of Excised Blood Vessel for Vascular Grafts

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:26 GMT

TechID: N2480 Coronary artery bypass grafting is one of the most frequent surgical procedures in the United States. There is a sizable unmet demand for effective small-diameter vascular prostheses for use in coronary bypass surgery. Currently, the best replacements for occluded arteries are autologous arteries, which have a cumulative patency rate of 93% after 5 years. Donor veins of appropriate dimensions are more readily available and are frequently used despite their substantially lower patency (45% after 5 years). The limited availability of suitable autologous arte...

A Simple General Method for Synthesis of Parent Polyaniline Oligomers by Oxidative Coupling Reaction (Combined into J1630)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:25 GMT

TechID: I1462 Researchers at the University of Pennsylvania have developed a novel simple technique for synthesizing polyaniline oligomers. The method consists of a series of oxidative reactions from which oligomers of arbitrary length may be synthesized. From this procedure, previously unreported polyaniline oligomers can be synthesized, including a "16-mer" and an octamer. Applications Advantages

Therapeutic Compounds for Blocking DNA Synthesis of Pox Viruses

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:22 GMT

TechID: T4367 Smallpox is a highly contagious and deadly infectious disease caused by a DNA virus, commonly known as poxvirus. Given the growing threat of bioterrorism and the increasing percentage of unvaccinated Americans, there exists an immediate need for non-toxic, vaccine-alternative, smallpox inhibitors that may be easily stockpiled. Poxvirus encodes its own proteins required for lytic viral replication, including a DNA polymerase (E9) and accessory proteins (A20 and D4R). The E9 DNA polymerase forms a highly specific complex with A20 and D4R, essential for viral DN...

Gene Encoding Novel Multispecific Heavy Metal Resistance Factor from Plants

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:20 GMT

TechID: K1879 Excessive amount of heavy metals, such as cadmium, arsenate, mercury, lead, copper, and zinc, can pose an acute problem for all living organisms. Years of industrial and mining activities have contaminated ground water and soils with heavy metals, which can be taken up by crop plants and eventually by humans. Therefore, there is an urgent need for environmental cleanup to prevent heavy metals from entering the food chain. Compared to conventional physical and chemical methods for environmental cleanup, phytoremediation employs plants to extract heavy...

Recombinant Listeria Monocytogenes Vaccine and Therapeutic Methods Employing Same

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:19 GMT

TechID: D751 Listeria monocytogenes is a gram-positive, food-borne human and animal pathogen responsible for infection in immunocompromised individuals and pregnant women. L. monocytogenes enters the host cytoplasm and uses a host system of actin-based motility to mediate movement both within a cell and from cell-to-cell without leaving the cytoplasm. [This method of intracellular movement bypasses the humoral immune system of the host organism and avoids cell mediated immunity response.] Vaccines against such parasites have been ineffective due to the difficulty of inducing in viv...

New Entranceway Design for Rail Passenger Cars

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:18 GMT

TechID: N2544 Different types of railroad station platforms are not always matched by an appropriate passenger car entranceway. This results in fatalities and injuries to passengers boarding and alighting trains, prolongs train dwell times at stations, and requires significant time and effort of the train crew, increasing the cost of train operation. Researchers at the University of Pennsylvania have developed a new entranceway design, presented below, that accommodates all types of platforms with little effort on the part of the train crew. Many railroads in the US have both h...

Neurogenin-3 GFP Mice

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:17 GMT

TechID: M2321 Researchers at the University of Pennsylvania have developed genetically altered strain of mice, transgenic mice, that carries a copy of the enhanced green fluorescent protein (EGFP) in the mouse neurogenin-3 locus. This mouse was engineered by homologous recombination in mouse embryonic stem cells and represents a "knock-in" of the EGFP gene into the Neurogenin-3 locus. Neurogenin-3 has been shown in laboratory studies to be expressed in the earliest population of endocrine stem cells in the pancreas. Mice carrying this Neurogenin-3 EGFP allele express green fl...

Herpes Simplex Virus Type 1 Glycoprotein E Domains Involved in Virus Spread and Disease

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:13 GMT

TechID: S4034 Researchers modified wild-type HSV-1 virus by point mutation or insertion mutation, to determine which motifs, domains, and residues are important in the binding of IgG-Fc. This binding is important in determining the extent of viral spread in an organism. Results indicated that. The insertion mutation at a/a 380 significantly impaired the ability of the virus to spread to dorsal root ganglia, and from ganglia back to skin. PCR indicated that the domain surrounding a/a 380 is important for bding and thus viral spread, and represents a potential target for antiviral th...

A Method for Relating Pulmonary Ventilation-Perfusion Ratios to Pressure-Flow Curves in Lungs

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:08 GMT

TechID: G1092 In diagnosing and treating pulmonary problems, a doctor determines the most probable underlying cause of the patient’s symptoms based on a variety of diagnostic measurements. Physicians have an idiosyncratic tendency to weigh some factors more heavily than others. Currently, two separate techniques are used to analyze lung function. The first measures pulmonary gas exchange in a ratio known as the ventilation/perfusion ratio. The second measures the pressure/flow relationship of pulmonary blood circulation. This invention consists of computer software and hardwa...

Macrophage-Tropic HIV

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:06 GMT

TechID: F1085 Human immunodeficiency virus type 1 (HIV-1) strains may be divided into categories depending upon the cell in which they replicate and the stage of disease in which they occur: macrophage-tropic variants are found early in infection, replicate in primary macrophages and lymphocytes but not T cell lines, and are relatively non-cytopathic (kill lymphocytes relative poorly). In contrast, T cell tropic variants develop in infected individuals as disease progresses, replicate in lymphocytes and T cell lines but not macrophages, and are highly cytopathic. Macrophage-tr...

cDNA for alpha-7 Subunit of Human Nicotinic Acetylcholine Receptor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:19:04 GMT

TechID: N2614 Alpha-7 nicotinic acetylcholine receptors (AChRs) are the primordial of many subtypes of AChRs. AChRs are acetylcholine-gated cation channels which function as classic postsynaptic neurotransmitter receptors in skeletal muscle, but which in other tissues may serve other functional roles. Alpha-7 AChRs are found in presynaptic, postsynaptic, and extrasynaptic locations in neurons and they have been found in glia and non-neuronal tissues. Research studies have implicated Alpha 7 in protein neurotoxic effects of Alzheimer's disease, the neuroprotective effects of nicotine, and ...

Beta-1 and Alpha-6 Integrin are Surface Markers on Mouse Spermatogonial Stem Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:59 GMT

TechID: L2060 Self-renewing stem cells, such as spermatogonial stem cells have generated interest in the field of cellular regeneration. These cells have the capability of producing progeny cells that differentiate into fully functional spermatozoa after transplant. However, due to a lack of biochemical and surface antigen markers for spermatogonial stem cells, biological studies in this field have been severely handicapped. University of Pennsylvania researchers have developed a novel method of purifying spermatogonial stem cells and identifying its associated molecular marker. The pur...

Immunotargeting of Plasminogen Activator(s) to the Pulmonary Endothelium

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:58 GMT

TechID: H1262 Acute and chronic thrombotic states are major causes of morbidity and mortality in many industrialized countries. Occlusion of blood vessels by thrombus or fibrin clots plays an integral role in both heart and pulmonary disease. One approach to the treatment of an established thrombosis is the pharmacological dissolution of the blood clot via intravenous infusion of plasminogen activators. The researchers at the University of Pennsylvania have developed a novel technique that allows delivery of tissue-type plasminogen activator (t-PA) specifically to...

Compositions which are Immunologically Crossreactive With Antibodies and Preparative Methods Therefor

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:55 GMT

TechID: D771 Many pathogens attack host cells through receptor-mediated interactions. Previous attempts to synthesize compounds (i.e. antibodies) against such receptors in an effort to block pathogen binding have met with difficulty because such compounds were, themselves, immunogenic. University of Pennsylvania researchers have developed synthetic non-immunogenic compounds which mimic the complementarity determining region (CDR), the antigen-binding region, of antibodies against receptors important to pathogen attack. The compounds possess an identical conformation to ...

Scale-Based Image Filtering Methods Preserving Boundary Sharpness and Fine Structure

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:50 GMT

TechID: M2242 Current MRI protocols yield low signal to noise ratio (SNR) and low contrast-to noise ratio (CNR,) resulting in poor image acquisition. A number of initiatives have been established to address this problem. For example, post acquisition filtering is a major off-line image processing technique used to improve SNR and CNR. Unfortunately, post acquisition filtering often diffuses or blurs important structures. [An alternative is to use 2D or 3D imaging techniques which acquire images at a higher resolution. However, such methods increase the acquisition time. Moreover, image ar...

10 Gene Classifier for Squamous Cell Carcinoma

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:48 GMT

TechID: S4090 Researchers at University of Pennsylvania and The Wistar Institute have developed a panel of ten genes that is a highly-accurate tool for distinguishing between primary lung cancer and metastatic squamous cell carcinoma of the lung in patients with previous head and neck malignancies. They developed the 10-gene classifier panel from among 100 high-potential candidate genes identified using more than 9,000 probes. This integrative biomarker tool has been validated on data from four independent laboratories on more than 120 samples of primary lung squamous...

HIV-1 (Human Immunodeficiency Virus Type 1) Strain

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:46 GMT

TechID: J1492 HIV-1 strains are categorized according to the cell types in which they infect and replicate. Typically, these fall into two categories, T-cell tropic and macrophage-tropic. University of Pennsylvania researchers have isolated and molecularly cloned the first strain that is dual-tropic, and thus is able to enter both macrophages and T-cell targets. The reason for this is that it is able to utilize both the macrophage-tropic HIV-1 entry cofactor CCR5 and the T-cell line-tropic entry cofactor CXCR-4. It also uses several other entry cofactors such as CC...

A Calculus for Collections and Aggregates

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:42 GMT

TechID: L1913 Traditional query language compilers often consume massive amounts of memory and occupy large amounts of I/O resources. Complex queries also often require construction of intermediate data structures, which further consumes memory. Increasing sizes of databases and information demands have made many queries unfeasible without some sort of optimization. While most compilers for general-purpose programming languages perform some optimizing, they do not generally perform this function for database queries...

Effects of Nonlipolytic Ligand Function of Endothelial Lipase on High Density Lipoprotien Metabolism in Vivo

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:41 GMT

TechID: Q3585 Endothelial lipase (EL) influences high density lipoprotein (HDL) metabolism in vivo and mediates bridging and uptake of HDL particles independent of its lipolytic activity in vitro. The investigators at University of Pennsylvania have developed a catalytically inactive mutant by changing the serine 149 active site of EL to alanine (ELS149A) (Uli, et al., JBC, 278, 40688-40693, 2003). They found that EL has both a lipolytic and nonlipolytic function in HDL metabolism in vivo and lipolytic activity of EL is most important for its effects on systemic HDL metabolism.&nb...

Ion Channel Complex Auxiliary Proteins for Male Contraceptive Development

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:39 GMT

TechID: S4295 Ion channels control ion flux and play primary roles in the regulation of sperm cell behavior and male fertility, and as such ideal targets for non-hormonal male contraceptive drugs. The most promising sperm ion channel targets are the CatSper channels (CatSper1-4), expressed in testis. CatSper1 or 2 deficient human males are infertile. These data demonstrate that drugs specifically blocking CatSper channels can be potent male contraceptives without side effects. The current development of drugs targeting CatSper, however, is hindered by the failure to r...

Resistance Switching Memory Device

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:25 GMT

TechID: T4407 Current non-volatile memory market, counts about a third of the entire $60B memory market, is dominated by flash memories. However, existing flash memory technology is facing challenges of unable to scale down to 22-nm, the cell size projected for year 2016 memory market. According to a paper published in Science (Vol 317 (2008) P1625), resistance-witching cell is one of the most promised contenders for replacing flash cells. Resistance-switching-type memories have been studied for many years, o...

HIV-MSCV Hybrid Vector for High Expression in Non-dividing Hematopoietic Cells

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:15 GMT

TechID: L2001 The murine stem cell virus (MSCV) based retroviral vector is an effective vehicle for delivering and expressing exogenous genes in human hematopoietic progenitor cells. A major limitation to this system is that viral integration requires cell division. This issue is typically addressed by optimizing culture conditions for maximal cell replication. Unfortunately, while this strategy significantly augments efficiency of progenitor cell infection, it does not solve the problem of poor transduction of primitive, non-cycling stem cells, which are often the desir...

Research in High Resolution Ultrasound

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:12 GMT

TechID: I1474 Acoustic wavefront distortion caused by propagation of coherent waves inside the inhomogeneous tissue produces similar effects as noise upon images. Filtering a noisy signal is a well established procedure to enhance the usable information content of the signal. Inverse filtering and matched filtering are two examples of such filtering approaches. Inverse filtering enhances image resolution, but is sensitive to certain stability problems. Matched filtering improves the signal-to-noise ratio which is an optimum solution for detection as in radar...

Function and Regulation of ADAMTS-1

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:10 GMT

TechID: Q3546 Tumor progression is marked by tumor angiogenesis and the survival and proliferation of tumor cells. Current chemotherapies typically target the carcinoma cells, but there is a need for new therapies that will attack both carcinoma cells and tumor-associated angiogenesis. Researchers at the University of Pennsylvania have characterized the ADAMTS-1 protein, which may serve as a novel anti-cancer target and whose fragments act as anti-cancer reagents. Full-length ADAMTS-1 (A Disintegrin And Metalloproteinase with ThromboSpondin Motifs) promotes tum...

Improved Single Component Solid Oxide Fuel Cell

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:09 GMT

TechID: H1321 Solid oxide fuel cells (SOFC) hold some inherent advantages over other fuel cells such as molten carbonate, phosphoric acid (high temperature operation; corrosive) or membrane types. SOFC’s typically possess efficiencies approaching 80%. They require less energy input for specific power load. SOFC have suffered in the past from relatively low energy density and fabrication problems. For instance, the laminated structure of older SOFC’s often results in delamination after several use cycles; differential expansion of the laminate results in dela...

Pregnancy Up-Regulated Nonubiquitous Cam Kinase

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:07 GMT

TechID: O2740 PNCK is a novel CaM multi-functional protein kinase which is overexpressed in a subset of primary breast cancers. PNCK is associated with the correlation between carcinogenesis, and postnatal development, and PNCK expression is specifically restricted to a subset of mammary epithelial cells. PNCK is expressed in an oncogene-associated manner in mammary tumor cell lines with defined genetic initiating events, and is restricted to a subset of human breast cancer tumors. PNCK expression is limited to a subset of ductal carcinomas. Data indicates that ...

Precursors for F18-fluorination of 2-Nitroimidazole Hypoxia Markers

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:06 GMT

TechID: L2057 Many human and animal diseases are characterized by the pathological formation of tissue hypoxia and ischemia. The preferred treatment of these conditions is often dependent upon the extent of hypoxia in the affected tissues of individual patients. The novel compounds EF5 and EF3 are currently undergoing clinical trials to study their effectiveness as a hypoxia marker. The detection methods are presently based on the immunohistochemical identification of the metabolized drug in cells or tissues. It is desirable to extend the use of these drugs ...

Human and Rat Heart and Skeletal Muscle Voltage-Sensitive Sodium Channels

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:05 GMT

TechID: J1521 Penn researcher Dr. Roland Kallen has developed certain rat and human skeletal muscle and cardiac sodium channel cDNAs in expression vectors suitable for Xenopus laevis oocyte or mammalian cell expression (e.g.. the Human Embryonic Kidney 293 cell line). Dr. Kallen has also developed a stable CHO cell line expressing the human cardiac sodium channel for screening based on electrophysiological or drug binding technologies. Relevant Publications: “Primary Structure and Functional Expression of a Mammalian Skeletal Muscle Sodium Channel." Trimmer, J.S., Cooperman, S.S., Tom...

Processes and Intermediates for Preparing Macrocycles (Demethoxyrapamycin)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:18:04 GMT

TechID: H1259 Rapamycin, a protein Ser/Thr kinase molecule produced naturally by the bacterium S. hygroscopicus, has both antibiotic and immune suppressant properties. Its use as a transplant rejection prophylaxis is covered under use patents and marketed by Wyeth-Ayerst under the brand name Rapamune. In addition to the above FDA-approved use of rapamycin, its analogues appear to be useful in the treatment of psoriasis and fungal infection. The drug is conventionally produced via expensive biosynthesis with the producing organism, a process with low yield. Researc...

An Attenuated Strain of Listeria Monocytogenes for Use as a Vaccine Vector

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:59 GMT

TechID: J1598 Because cytotoxic T cells (CTLs) can potentially destroy HIV infected cells and might prevent the dissemination of the infection at early times in an infected individual, generation of a strong CTL response may be useful as a prophylactic or therapeutic vaccine against HIV. Researchers at the University of Pennsylvania have proposed a novel method for generating strong immune responses against HIV wherein HIV antigens will be produced inside of a vaccinated host. The delivery vector for the genes encoding for these antigen proteins will be a facultative in...

Serotonin-1A Ligands and Imaging Agents

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:58 GMT

TechID: G1137 University of Pennsylvania researchers have developed a series of tetralin derivatives (i.e., PIPAT) that selectively and strongly bind to specific central nervous system receptors, such as the dopamine-d3 receptor and the serotonin-5-HT receptor. These PIPAT derivatives can be radiolabeled with iodine and then used as diagnostic agents in SPECT or PET imaging. The compounds may be used as diagnostic brain imaging agents or they may be useful in the identification and evaluation of therapeutic agents that act at the serotonin and dopamine recep...

Light Hydrocarbon Separation Using ITE-type Zeolites

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:56 GMT

TechID: M2324 Background of the invention The separation of propylene from propane is an important and large-scale commercial process in the petrochemical industry. Traditionally, the separation is performed in distillation columns containing about 125 plates, making it the most cost- and energy-intensive separation process in this industry. Researchers at the University of Pennsylvania have created an adsorption-based propane/propylene separation method that results in the creation of a much more energy- and cost-effective industrial-scale separation process. Technology descriptio...

Method for Tolerizing a Mammalian Patient to Administration of Gene Therapy Virus Vectors

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:53 GMT

TechID: J1649 There exists a need in gene therapy to induce specific immunologic tolerance to viral vector capsid proteins when these vectors are used to introduce both viral gene and transgene products into mammalian cells. Researchers at the University of Pennsylvania have developed a method for eliminating the immune barrier impeding long-term gene therapy by selectively tolerizing a mammalian subject to multiple administrations of a live recombinant virus carrying a gene for delivery to a cell of said subject. This method involves administering to the subject a suitable a...

Design of degenerate PCR primers to generate single chain Fragment variable antibodies for targeted therapy in the dog

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:49 GMT

TechID: U4763 Researchers from the University of Pennsylvania, School of Veterinary Medicine have developed a Platform Technology and/or Research tool that is intended for use in the development of canine compatible antibodies or antibody fragments. These antibodies are intended for use in targeted therapy of cancer as well as viral and bacterial targeting and neutralization and toxin binding. Cancer is the leading cause of death in canine pets. The mainstay treatment for cancer is systemic combination ch...

In Situ Proteomics in Cells and Tissue Sections (CAQE)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:36 GMT

TechID: O2647 One of the most useful diagnostic tools in research and medicine is the ability to detect and quantify proteins on tissue sections. Faculty at the University of Pennsylvania have developed a methodology called Consecutive Addition of Quantifiable Extenders (CAQE) that enables the analysis of thousands of proteins simultaneously in situ and is an easily-automated iterative procedure. While exemplified on antibodies, this method can also be used with ScFV’s, CDR’s, or other proteins or agents that will b...

Multispectral Omnidirectional Sensor (MOOSE)

jharris@ctt.upenn.edu — Fri, 13 Nov 2009 12:17:34 GMT

TechID: N2476 MOOSE, Multispectral Omnidirectional Sensor, is a real-time video camera with a 360 degrees field of view and robust capabilities of performing under any weather and visibility conditions. Proprietary camera design and image processor provide live surveillance in all directions in smoky, rainy, foggy conditions at any time of day or night. The camera does not have any moving parts and captures the entire field of view at once, giving the operator a full view of the area under surveillance. The image processor can be implemented in software and/or hardware, providing t...