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Solid State Nanopore DNA Sequencing

Description:

 

Inventor

Dr. Marija Drndi?

Problem

Existing sequencing processes require DNA to be copied millions of times and labelled with fluorescent tags. This procedure can require eight days of processing time at a cost of thousands of dollars. The slow and expensive process presents a barrier to screening patients’ genomes and inhibits the rise of personalized medicine.

 

Solution

Dr. Marija Drndic’s team has focused on DNA sequencing using solid state nanopores. DNA bases pass through a small hole in a thin graphene or silicon membrane, and the change in electrical conductance across the pore can be measured and correlated to the DNA base sequence. The technology portfolio in this area includes Transmission Electron Beam Lithography techniques to efficiently and effectively create thin membranes for nanopore devices, as well as the design of the devices themselves.  Complete nanopore devices fabricated using these techniques have been used to monitor and detect DNA translocation through the pores. Additional applications include detecting and identifying RNA molecules, proteins, and other biomolecules using the nanopore technique.

 

Advantages

•       High speed and low cost DNA sequencing

•       Improved performance as compared to protein membrane devices

 

Stage of Development

Prototype systems developed

  

Intellectual Property

US Patents 8,039,368, 8,173,335 & 9,121,823. Multiple pending patent applications.

 

Reference Media

Drndi? et al. (2010). Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors. Nature nanotechnology, 5(11), 807-814.

http://www.nature.com/nnano/journal/v5/n11/abs/nnano.2010.202.html

Fischbein, M. D., & Drndic, M. (2007). Sub-10 nm device fabrication in a transmission electron microscope. Nano letters, 7(5), 1329-1337.

http://pubs.acs.org/doi/abs/10.1021/nl0703626

 

Desired partnerships

•  License

Co-development

 

 

Download PDF

 

Docket# W5451

 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Marija Drndic
Keywords: