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Biorealize: a customized platform for high-throughput, automated cell culture

Description:

Integrated desktop system for biological design and culture of genetically modified organisms

 

Inventor

Orkan Telhan, Assistant Professor of Fine Arts - Emerging Design Practices;
Karen Hogan, Department of Biology Lab Coordinator;
Mike Hogan 

 

Technology Overview

Researchers in the School of Design and Department of Biology have collaborated to design a desktop suite of novel devices and methods for high-throughput, automated liquid cell culture.  There are innovations in bioreactor design, liquid transfer, and process analytics to incorporate transfection, incubation, DNA purification, and isolation in one system. The design of genetically modified organisms (GMOs) is readily approachable in combination with the existence of DNA design programs.  Because of the small footprint of the design, this system can appeal to non-academic use without the complexity and cost of a traditional cell culture facility.  Because cells are not exposed to the user or environment before lysis, the safety hazards and culture contamination risks associated with traditional cell culture are minimized. Through advanced process analytics, the system can carry out parallel experiments that explore different DNA designs, as well as variations in culture media and process conditions (i.e., humidity, temperature, pH) with combinatorial design methods. By providing a standardized hardware and software platform, researchers are able to reproduce findings and validate each other’s protocols. 

 

 

Advantages

•       Closed, safe, asceptic research platform

•       Process automation, analytics, and history

•       Low cost, portable, small footprint system

•       Minimize culture contamination

•       Minimize amount of glassware / autoclaving / consumables / transfer operations/human intervention

•       Compatible with standard interfacing consumables, protocols, plasmids, cell lines

•       Carry out multiple experiments simultaneously

 

Applications

•       Automated liquid cell culturing  for aerobes and anaerobes

•       Design of genetically modified organisms

•       Reproducing and verifying standardized biological parts (i.e., Plasmids, BioBricks)

•       Manipulating interplay between culture conditions and genetic expression

•       Combinatorial experimentation with different DNA designs and culture conditions

•       Wide variety of fields, including synthetic biology, bioengineering, food/flavor/fragrance research, education, design, materials

 

Reference Media

Website

Popular Science article

Philadelphia Inquirer article

Highlight in Forbes

 

Stage of Development

•       Proof-of-concept, Prototype

  

Intellectual Property

PCT pending

 

 

Desired partnerships

• License

Co-development

 

 

Download PDF

 

Docket #  15-7445 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Orkan Telhan
Karen Hogan
Michael Hogan
Keywords:
Biomedical Engineering
Bioplastics
Biotechnology
Education & Training