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Rapid plasma separator for point-of-care diagnostics and viral load detection that does not require electrical power

Description:

Membrane-based, sedimentation-assisted plasma separator from whole blood for clinical tests

 

Inventor

Haim H. Bau, Professor of Mechanical Engineering and Applied Mechanics

Changchun Liu, Assistant Research Professor

Michael Mauk, Visiting Assistant Clinical Professor

 

Problem

High sensitivity, point-of-care clinical tests, such as HIV load, require large volumes of plasma.  Centrifugation is widely used for separating plasma from whole blood, but it is not always accessible and can be a limiting factor for point-of-care testing.  Current microfluidic systems require extensive blood dilution that adversely affects the limit of detection, which is critical in high sensitivity tests such as viral load detection.

 

Solution

Using size exclusion-based filtration and gravitational sedimentation, researchers in the Bau lab have developed a plasma separator capable of separating plasma from undiluted whole blood within minutes.  The plasma separator consists of an asymmetric, porous, polysulfone membrane housed in a disposable chamber.  The vertical design of the device avoids membrane clogging and enables the extraction of plasma volumes without a pump and excessive dilution or wash. The device is fabricated from inexpensive, disposable plastics with a footprint no bigger than a dime.  Additionally, the device uses no electricity and has no moving parts.

 

 

Advantages

•       Low-cost (<$1/test)

•       Rapid process

•       Easy to administer

•       No requirement for electrical power

•       Extract large volumes of plasma without clogging

•       Self-terminating separation process to prevent hemolysis

 

Applications

•       Separate plasma from blood

•       Detect viral load when combined with nucleic acid testing on microfluidic chip

•       Use in resource-poor regions without trained staff, laboratory facilities, or electricity

 

 

Stage of Development

Prototype developed and in vitro proof-of-concept testing 

 

Intellectual Property

PCT pending (WO2015095491 A1)

 

Reference Media

Liu et al.  Analytical. Chemistry, 2013, 85 (21), p. 10463–10470.

 

Desired partnerships

•  License

Co-development

 

 

Download PDF

 

Docket #  14-6806

 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Haim Bau
Changchun Liu
Michael Mauk
Keywords:
Amino Acid
Engineering