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Carbon-based nanopipettes for single cell injection

Description:

Multiplexed, nanoscale electrochemical sensor for analyte delivery and detection

 

Inventor

Haim H. Bau, Professor of Mechanical Engineering and Applied Mechanics

 

 

 

Technology Overview

The Bau lab has developed carbon-based nanopipettes (CNPs) that allow for reliable single cell injection.  These CNPs use an AC electrical impedance measurement to detect cell and nucleus penetration, which greatly improves upon standard pulled glass pipette injections.  The small dimensions of the nanopipette enhance temporal resolution.  Small volumes can be injected in a minimally invasive, controlled manner.  Because carbon lining is conductive, CNPs can be used as accurate biosensors.  This improved platform for single cell injection allows for automated, high-throughput methodology.  This technology has been successfully demonstrated with fluorescent tRNA transfection of both the cytoplasm and nucleus of single cells.

 

Advantages

•       Small dimensions, minimally invasive, does not break or clog easily

•       Compatible with biomaterials, micromanipulators, micropipette fittings, and amplifiers

•       Accurate injection into cytoplasm and/or nucleus

•       Mass producible

 

Applications

•       Single cell injection of probes, oligonucleotides, tracers, fluids, transfer of nucleic acid populations

•       Injection of very small amounts

•       Biosensors

•       Reliable, automated high-throughput platform for cell injection

 

 

Stage of Development

•       In vitro testing

  

Intellectual Property

US patent 8,702,927

US patent 8,877,518

 

Reference Media

Anderson SE et al.  Nanotechnology, 2015, 26(18), 185101.

Rees HR et al.  Analytical Chemistry, 2015, 87, 3849-3855.

Anderson SE et al.  Nanotechnology, 2014, 25(24), 245102.

 

Schrlau et al. J. of the Assoc. for Lab Automation, 2010, 15(2), p. 145-151.

Schrlau et al.  ACS Nano, 2009, 3(3), p. 563-568.

Liu et al.  Biosensors and Bioelectronics, 2009, 25(4), p. 809-814.

Schrlau et al.  Microfluidics and Nanofluidics, 2009, 7(4), p. 439-450.

Schrlau et al.  Nanotechnology, 2008, 19(1), p.015101.

 

Desired partnerships

•  License

Co-development

 

 

Download PDF

 

Docket #  V5030 and T4321 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Haim Bau
Keywords:
Engineering
Nanotechnology