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High efficiency, superhydrophobic clamshell plasma separator for point-of-care diagnostics

Description:

Membrane-based, sedimentation-assisted plasma separator 

 

Inventor

Haim H. Bau, Professor of Mechanical Engineering and Applied Mechanics

Changchun Liu, Assistant Research Professor

Michael Mauk, Visiting Assistant Clinical Professor

 

Problem

High sensitivity, point-of-care clinical tests, such as HIV viral load, require large volumes of plasma extracted from whole blood.  Centrifugation is widely used for separating plasma, but it is not always accessible in resource-poor settings.  Current microfluidic systems require extensive blood dilution that adversely affects the limit of detection, which is critical in high sensitivity tests such as viral load detection.

 

Solution

The Bau lab has developed a point-of-care device for highly efficient, rapid plasma separation using a superhydrophobic material and gravitational sedimentation.  This is the second generation plasma separator device from the lab.  The plasma separator has a clamshell design, with a superhydrophobic top cover and bottom substrate.  In order to increase the membrane separation capacity and plasma yield, the separation membrane is at the top of the whole blood film.  By adding the superhydrophobic coating, surface bioadhesion of the blood is minimized, the blood clotting time is increased, and blood cell hemolysis is reduced.  Furthermore, the loss of target biomolecules is prevented, with high recovery efficiency.  This device can be used without external instrumentation and in resource-poor regions without electricity or specially trained medical personnel.

  

 

Advantages

•       Low-cost and disposable

•       Rapid process (<10 min)

•       Easy to administer

•       No requirement for electrical power

•       Minimal surface adhesion and blood cell hemolysis

 

Applications

•       Separate plasma from whole blood

•       Detect viral load when combined with nucleic acid testing on microfluidic chip

•       Use in resource-poor regions without trained staff, laboratory facilities, or electricity

 

Stage of Development

Prototype developed and in vitro proof-of-concept testing

 

Intellectual Property

PCT pending (WO 201595491 A1)

 

Reference Media

Liu et al.  Lab on a Chip, 2016, 16, p. 553-560.

Video highlight.

Liu et al. Anal. Chem., 2013, 85 (21), p. 10463–10470.

Penn News highlight.

 

Desired partnerships

• License

Co-development

 

Download PDF

 

Docket #  14-6973 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Haim Bau
Changchun Liu
Michael Mauk
Keywords:
Engineering