Available Technologies

Browse Penn-owned technologies available for licensing.


Transcriptome-wide RNA-protein interactions


RNAase-mediated protein footprint sequencing to reveal protein binding sites on transcriptome-wide scale



Brian Gregory, Assistant Professor of Biology 


Technology Overview

The Gregory lab has developed a high-throughput, ribonuclease-mediated protein sequencing approach termed Protein Interaction Profile sequencing (PIP-seq).  PIP-seq identifies RNA-protein interaction sites within both unprocessed and mature RNAs in a predominantly unbiased manner and on a transcriptome-wide scale.  Multiple cross-linking techniques capture both direct and indirect RNA-protein interactions.  PIP-seq has revealed that both single- and double-stranded RNases uncover distinct but overlapping sets of RNA-protein interaction sites.  This is a reproducible sequencing approach capable of revealing known and unknown RNA-protein interactions.  The researchers have applied PIP-seq to both the human and plant transcriptomes, identified RNA-binding protein motifs, and uncovered a significant enrichment for disease-associated polymorphisms within RNA-binding protein interaction sites. 



•       Apply with cell culture, tissue, or whole organisms

•       Captures large multiprotein complexes of RNA architecture

•       Independent of incorporation of unnatural nucleotides or UV cross-linking methods



•       Uncover transcriptome regulatory processes by global assessment of RNA-protein interactions

•       Gene expression studies to identify single nucleotide polymorphisms associated with disease variants


Stage of Development

•       In vitro testing 


Intellectual Property

USSN 9,097,708


Reference Media

Penn News highlight on Gregory lab.

Gosai SJ et al.  Molecular Cell, 2015.  57, p. 376-388.

Silverman IM et al.  Methods, 2015, 71:76-85.

Silverman IM et al.  Genome Biology, 2014, 15(R3)


Desired partnerships

•  License




Download PDF


Docket #  Y6152 

Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
Brian Gregory