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Mutant cytosine modifying enzymes to enhance epigenetic or base-editing on genomic DNA

Description:

Mutant TET enzymes that stall the oxidation reaction of 5-methylcytosine (5mC) at 5-hydroxymethylcytosine (5hmC), and AID/APOBEC enzymes with hyperactive deamination activity.

 

Inventors

Rahul Kohli, M.D., Ph.D., Christopher Nabel, Emily Schitsky, Ph.D, Monica Yun, Ph.D

 

Problem

Epigenetic modifications to DNA, such as cytosine methylation, play critical roles in modulating gene expression without changing coding sequences. Changes or editing of bases is also critical in immune defense. Localizing of targeting modifications or mutations is important to understanding or manipulating physiological and pathological processes.

 

Solution

Dr. Kohli and his colleagues at University of Pennsylvania developed mutant cytosine modifying enzymes; specifically, AID (activation-induced cytidine deaminase), APOBEC (apolipoprotein B editing complex), and TET (ten eleven translocation) enzymes with improved functions.  Hyperactive AID or hyperactive APOBEC proteins can be used in APOBEC-coupled epigenetic sequencing (ACE-Seq) or other epigenetic sequencing methods to distinguish cytosine from modified cytosine bases.  TET mutants are useful for stalling oxidation reactions at hmC and/or introducing hmC modifications into specific sites in the genome.  In addition to epigenetic applications, these mutant enzymes can be valuable in genome or epigenome editing in combination with CRISPR or other tools for targeting. 

 

 

 

Figure. TET enzymes sequentially oxidize 5-methylcytosine (mC) into three additional bases: 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxylcytosine (caC). AID/APOBEC enzymes deaminate cytidine bases to uridine analogs and preferentially act on ssDNA.  The DNA modifications by these enzymes are critical for many physiological actions.

 

Figure from Schutsky et al. Nucleic Acids Res, 2017, 45 (13) – 7655.  

 

Applications

• Sequencing

• Gene editing

• Epigenome editing

 

Advantages

• More controllable enzymes.

• Increased base-editing efficiency and precision.

 

Stage of Development

• Demonstrated applications in sequencing

• Ongoing development of genome and epigenome editing applications

 

Intellectual Property

• US utility patent application filed

 

Reference Media

       Gajula et al. Nucleic Acids Res, 2014, 42 (15) – 9964

       Liu et al. Nat Chem Biol, 2017, 13 (2) – 181

 

Desired Partnerships

1. License

2. Co-development

 

Docket #: Z6653


Patent Information:
For Information, Contact:
Jessica Casciano
University of Pennsylvania
casciano@upenn.edu
Inventors:
Rahul Kohli
Christopher Nabel
Emily Schutsky
Monica Yun
Keywords: