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Carbon-based nanopipettes for single cell injection

Description:

Multiplexed, nanoscale electrochemical sensor for analyte delivery and detection

 

Technology Overview: 

The Bau Lab has developed carbon-based nanopipettes (CNPs) that allow for reliable single cell injection. These CNPs use an AC electrical impedance measurement to detect cell and nucleus penetration, which greatly improves upon standard pulled glass pipette injections. The small dimensions of the nanopipette enhance temporal resolution. Small volumes can be injected in a minimally invasive, controlled manner. Because carbon lining is conductive, CNPs can be used as accurate biosensors. 

 

This improved platform for single cell injection allows for automated, high-throughput methodology. This technology has been successfully demonstrated with fluorescent tRNA transfection of both the cytoplasm and nucleus of single cells.

 

 

Inventor: 

Haim H. Bau, Professor of Mechanical Engineering and Applied Mechanics

 

Advantages: 

  • Small dimensions, minimally invasive, does not break or clog easily
  • Compatible with biomaterials, micromanipulators, micropipette fittings, and amplifiers
  • Accurate injection into cytoplasm and/or nucleus
  • Mass producible

 

Applications: 

  • Single cell injection of probes, oligonucleotides, tracers, fluids, transfer of nucleic acid populations
  • Injection of very small amounts
  • Biosensors
  • Reliable, automated high-throughput platform for cell injection

 

Stage of Development: 

In vitro testing

  

Intellectual Property: 

 

Reference Media: 

 

 

Desired Partnerships: 

  • License
  • Co-development

Docket # V5030 and T4321

 

Download PDF

 

 


Patent Information:
For Information, Contact:
Joshua Jeanson
Associate Director, SEAS/SAS Licensing Group
University of Pennsylvania
jeanson@upenn.edu
Inventors:
Haim Bau
Keywords:
Engineering
Nanotechnology