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Isothermal nucleic acid amplification reactor with integrated solid state membrane
Researchers in the Bau Lab have developed a disposable microfluidic cassette with an integrated porous extraction flow-through membrane for purifying and concentrating nucleic acids from biological samples.
Products can be detected by monitoring the reaction in real-time with a portable fluorescent reader, a smartphone camera, or visually when a colorimetric dye is used. The utility of the device has been demonstrated by detecting the presence of HIV-1 in oral fluids with a limit of detection of 10 HIV particles.
From Liu et al, 2011. In (A), a photograph of the single-chamber microfluidic cassette with an integrated membrane. In (B), the cassette holder is equipped with a thin film heater, a thermocouple, and a seat for the detector. The fluorescent signal is excited and detected with the portable ESE optical detector in the inset.
Stage of Development:
Proof-of-concept testing with HIV detection in non-clinical samples; prototype constructed
Docket # X5677