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ELISA, ICC, IF, IP, WB
The N-terminal 580 amino acids of dFMR1 was cloned into a His tag- containing vector. The induced protein was purified using a His-binding resin column. The purified protein was dialyzed against phosphate-buffered saline (PBS) and used to immunize mice.
[ELISA, IF, IP, WB] -
Wan L, Dockendorff TC, Jongens TA, Dreyfuss G., Characterization of dFMR1, a Drosophila melanogaster homolog of the fragile X mental retardation protein., Mol Cell Biol. 2000 Nov;20(22):8536-47.
[ICC, IP, WB] -
Kwak JE, Drier E, Barbee SA, Ramaswami M, Yin JC, Wickens M., GLD2 poly(A) polymerase is required for long-term memory., Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14644- 9. Epub 2008 Sep 9.
[ICC, WB] -
Monzo K, Dowd SR, Minden JS, Sisson JC., Proteomic analysis reveals CCT is a target of Fragile X mental retardation protein regulation in Drosophila., Dev Biol. 2010 Apr 15;340(2):408-18. Epub 2010 Feb 1.
[IP, WB] -
Additional data available (unpublished)
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