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anti-gemin 3 (Mouse)
ELISA, ICC, IP, WB
ddx20, gemin 3
Anti-Gemin3 antibodies 12H12 was prepared by immunizing Balb/C mice with 6His-tag COOH-terminal domain of Gemin3 (from amino acids 368–548 cloned from human) purified from nickel chelation chromatography using Novagen His-Bind buffer kit.
[ELISA, ICC, IP, WB] -
Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G., Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems., J Cell Biol. 1999 Dec 13;147(6):1181-94.
[ICC, IP, WB] -
Zhang H, Xing L, Rossoll W, Wichterle H, Singer RH, Bassell GJ., Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons., J Neurosci. 2006 Aug 16;26(33):8622-32.
Mahmoudi S, Henriksson S, Weibrecht I, Smith S, Söderberg O, Strömblad S, Wiman KG, Farnebo M., WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies., PLoS Biol. 2010 Nov 2;8(11):e1000521.
Additional data available (unpublished)
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