Available Technologies

Browse Penn-owned technologies available for licensing.

MAJIQ and VOILA: the next generation for sequencing analyses

MAJIQ and Voila are two software packages that together detect, quantify, and visualize local splicing variations (LSV) from RNA-Seq data.


Over 90% of human genes undergo alternative splicing, resulting in different gene products, potentially conveying different function. This underlying complexity to gene expression is still not well understood and mapping splicing variations remains a substantial challenge.


Dr. Yoseph Barash and his lab have developed new methods that improve the detection and accuracy of mapping gene splicing variations, thus shining a light on previously poorly studied transcripts. These innovative tools, MAJIQ and VOILA, have the potential to identify variations associated with a multitude of processes ranging from gene function to disease.

  • MAJIQ Builder: Uses RNA-Seq (BAM files) and a transcriptome annotation file (GFF3) to define splice graphs and known/novel Local Splice Variations (LSV).
  • MAJIQ Quantifier: Quantifies relative abundance (PSI) of LSVs and changes in relative LSV abundance (delta PSI) between conditions w/wo replicates.
  • Voila: A visualization package that combines the output of MAJIQ Builder and MAJIQ Quantifier using interactive D3 components and HTML5. Voila creates interactive summary files with gene splice graphs, LSVs, and their quantification.


Desired Partnerships:

One click license
Patent Information:


Docket #: 16-7706

For Information, Contact:

Viviane Martin
University of Pennsylvania