Multiplexed, nanoscale electrochemical sensor for analyte delivery and detection

Technology Overview: 

The Bau Lab has developed carbon-based nanopipettes (CNPs) that allow for reliable single cell injection. These CNPs use an AC electrical impedance measurement to detect cell and nucleus penetration, which greatly improves upon standard pulled glass pipette injections. The small dimensions of the nanopipette enhance temporal resolution. Small volumes can be injected in a minimally invasive, controlled manner. Because carbon lining is conductive, CNPs can be used as accurate biosensors. 

This improved platform for single cell injection allows for automated, high-throughput methodology. This technology has been successfully demonstrated with fluorescent tRNA transfection of both the cytoplasm and nucleus of single cells.

Inventor: 

Haim H. Bau, Professor of Mechanical Engineering and Applied Mechanics

Advantages: 

• Small dimensions, minimally invasive, does not break or clog easily
• Compatible with biomaterials, micromanipulators, micropipette fittings, and amplifiers
• Accurate injection into cytoplasm and/or nucleus
• Mass producible

Applications: 

• Single cell injection of probes, oligonucleotides, tracers, fluids, transfer of nucleic acid populations
• Injection of very small amounts
• Biosensors
• Reliable, automated high-throughput platform for cell injection

Stage of Development: 

In vitro testing

Intellectual Property: 

• US patent 8,702,927
• US patent 8,877,518

Reference Media: 

• Anderson SE et al.  Nanotechnology, 2015, 26(18), 185101.
• Rees HR et al.  Analytical Chemistry, 2015, 87, 3849-3855.
• Anderson SE et al.  Nanotechnology, 2014, 25(24), 245102.

• Schrlau et al. J. of the Assoc. for Lab Automation, 2010, 15(2), p. 145-151.
• Schrlau et al.  ACS Nano, 2009, 3(3), p. 563-568.
• Liu et al.  Biosensors and Bioelectronics, 2009, 25(4), p. 809-814.
• Schrlau et al.  Microfluidics and Nanofluidics, 2009, 7(4), p. 439-450.
• Schrlau et al.  Nanotechnology, 2008, 19(1), p.015101.

Desired Partnerships: 

• License
• Co-development

Docket # V5030 and T4321

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